全文获取类型
收费全文 | 948篇 |
免费 | 59篇 |
专业分类
1007篇 |
出版年
2022年 | 8篇 |
2021年 | 17篇 |
2020年 | 8篇 |
2019年 | 19篇 |
2018年 | 14篇 |
2017年 | 12篇 |
2016年 | 21篇 |
2015年 | 24篇 |
2014年 | 41篇 |
2013年 | 61篇 |
2012年 | 47篇 |
2011年 | 46篇 |
2010年 | 27篇 |
2009年 | 33篇 |
2008年 | 43篇 |
2007年 | 58篇 |
2006年 | 40篇 |
2005年 | 34篇 |
2004年 | 40篇 |
2003年 | 48篇 |
2002年 | 40篇 |
2001年 | 33篇 |
2000年 | 44篇 |
1999年 | 31篇 |
1998年 | 12篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 9篇 |
1994年 | 9篇 |
1993年 | 10篇 |
1992年 | 18篇 |
1991年 | 15篇 |
1990年 | 23篇 |
1989年 | 20篇 |
1988年 | 16篇 |
1987年 | 10篇 |
1986年 | 3篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 3篇 |
1980年 | 5篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 5篇 |
1972年 | 2篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1966年 | 3篇 |
1965年 | 2篇 |
排序方式: 共有1007条查询结果,搜索用时 0 毫秒
51.
Function of RNA-binding protein Musashi-1 in stem cells 总被引:19,自引:0,他引:19
Okano H Kawahara H Toriya M Nakao K Shibata S Imai T 《Experimental cell research》2005,306(2):349-356
Musashi is an evolutionarily conserved family of RNA-binding proteins that is preferentially expressed in the nervous system. The first member of the Musashi family was identified in Drosophila. This protein plays an essential role in regulating the asymmetric cell division of ectodermal precursor cells known as sensory organ precursor cells through the translational regulation of target mRNA. In the CNS of Drosophila larvae, however, Musashi is expressed in proliferating neuroblasts and likely has a different function. Its probable mammalian homologue, Musashi-1, is a neural RNA-binding protein that is strongly expressed in fetal and adult neural stem cells (NSCs). Mammalian Musashi-1 augments Notch signaling through the translational repression of its target mRNA, m-Numb, thereby contributing to the self-renewal of NSCs. In addition to its functions in NSCs, the role of mammalian Musashi-1 protein in epithelial stem cells, including intestinal and mammary gland stem cells, is attracting increasing interest. 相似文献
52.
Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts 下载免费PDF全文
53.
54.
Occurrence of a factor(s) which can selectively inhibit ribosomal RNA synthesis in isolated neurula cells of Xenopus laevis was examined in oocytes, unfertilized eggs, and embryos of Xenopus laevis. It was found that acid-soluble materials from full-sized oocytes, white-banded mature oocytes, unfertilized eggs, and pregastrular embryos were all active in significantly reducing the relative ratio of the [3H]uridine incorporation into 18S and 28S ribosomal RNA to that into 4S RNA from the control value. These results suggest that the inhibitor appears in the terminal step of oogenesis and, hence, may be assumed as a maternal regulator. 相似文献
55.
Daniela C. Granato Mariana R. Zanetti Rebeca Kawahara Sami Yokoo Romênia R. Domingues Annelize Z. Arag?o Michelle Agostini Marcelo F. Carazzolle Ramon O. Vidal Isadora L. Flores Johanna Korvala Nilva K. Cervigne Alan R. S. Silva Ricardo D. Coletta Edgard Graner Nicholas E. Sherman Adriana F. Paes Leme 《PloS one》2014,9(5)
Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules. 相似文献
56.
Inhibition of RANKL‐dependent cellular fusion in pre‐osteoclasts by amiloride and a NHE10‐specific monoclonal antibody 下载免费PDF全文
57.
Role of Caribbean Islands in the diversification and biogeography of Neotropical Heraclides swallowtails 下载免费PDF全文
Delano S. Lewis Felix A. H. Sperling Shinichi Nakahara Adam M. Cotton Akito Y. Kawahara Fabien L. Condamine 《Cladistics : the international journal of the Willi Hennig Society》2015,31(3):291-314
Numerous hypotheses on the evolution of Neotropical biodiversity have stimulated research to provide a better understanding of diversity dynamics and distribution patterns of the region. However, few studies integrate molecular and morphological data with complete sampling of a Neotropical group, and so there has been little synthesis of the multiple processes governing biodiversity through space and time. Here, a total‐evidence phylogenetic approach is used to reconstruct the evolutionary history of the butterfly subgenus Heraclides. We used DNA sequences for two mitochondrial genes and one nuclear gene and coded 133 morphological characters of larvae and adults. A robust and well‐resolved phylogeny was obtained using several analytical approaches, while molecular dating and biogeographical analyses indicated an early Miocene origin (22 Mya) in the Caribbean Islands. We inferred six independent dispersal events from the Caribbean to the mainland, and three from the mainland to the Caribbean, and we suggest that cooling climates with decreasing sea levels may have contributed to these events. The time‐calibrated tree is best explained by a museum model of diversity in which both speciation and extinction rates remained constant through time. By assessing both continental and fine‐scale biodiversity patterns, this study provides new findings, for instance that islands may act as source of diversity rather than as a sink, to explain spatio‐temporal macroevolutionary processes within the Neotropical region. 相似文献
58.
Yang CC Kawahara Y Mizuno H Wu J Matsumoto T Itoh T 《Molecular biology and evolution》2012,29(5):1471-1479
Results from studies on the domestication process of Asian rice Oryza sativa have been controversial because of its complicated evolutionary history. Previous studies have yielded two alternative hypotheses about the origin(s) of the two major groups of O. sativa: japonica and indica. One study proposes a single common wild ancestor, whereas the other suggests that there were multiple domestication events of different types of wild rice. Here, we provide clear evidence of the independent domestication of japonica and indica obtained via high-throughput sequencing and a large-scale comparative analysis of two wild rice accessions (W1943 and W0106) and two cultivars (a japonica cultivar called "Nipponbare" and an indica cultivar called "Guangluai-4"). The different domestication processes of the two cultivar groups appear to have led to distinct patterns of molecular evolution in protein-coding regions. The intensity of purifying selection was relaxed only in the japonica group, possibly because of a bottleneck effect. Moreover, a genome-wide comparison between Nipponbare, Guangluai-4, and another indica cultivar (93-11) suggests multiple hybridization events between japonica and indica, both before and after the divergence of the indica cultivars. We found that a large amount of genomic DNA, including domestication-related genes, was transferred from japonica to indica, which might have been important in the development of modern rice. Our study provides an overview of the dynamic process of Asian rice domestication, including independent domestication events and subsequent gene flow. 相似文献
59.
Daisuke Kawahara Shuichi Ozawa Kazushi Yokomachi Toru Higaki Takehiro Shiinoki Yoshimi Ohno Yuji Murakami Kazuo Awai Yasushi Nagata 《Reports of Practical Oncology and Radiotherapy》2019,24(5):499-506
ObjectivesThe aim of the current study is to evaluate the accuracy and the precision of raw-data-based relative electron density (REDraw) and the calibration-based RED (REDcal) at a range of low-RED to high-RED for tissue-equivalent phantom materials by comparing them with reference RED (REDref) and to present the difference of REDraw and REDcal for the contrast medium using dual-energy CT (DECT).MethodsThe REDraw images were reconstructed by raw-data-based decomposition using DECT. For evaluation of the accuracy of the REDraw, REDref was calculated for the tissue-equivalent phantom materials based on their specified density and elemental composition. The REDcal images were calculated using three models: Lung-Bone model, Lung-Ti model and Lung-Ti (SEMAR) model which used single-energy metal artifact reduction (SEMAR). The difference between REDraw and REDcal was calculated.ResultsIn the titanium rod core, the deviations of REDraw and REDcal (Lung-Bone model, Lung-Ti model and Lung-Ti model with SEMAR) from REDref were 0.45%, 50.8%, 15.4% and 15.0%, respectively. The largest differences between REDraw and REDcal (Lung-Bone model, Lung-Ti model and Lung-Ti model with SEMAR) in the contrast medium phantom were 8.2%, ?23.7%, and 28.7%, respectively. However, the differences between REDraw and REDcal values were within 10% at 20 mg/ml. The standard deviation of the REDraw was significantly smaller than the REDcal with three models in the titanium and the materials that had low CT numbers.ConclusionThe REDcal values could be affected by beam hardening artifacts and the REDcal was less accurate than REDraw for high-Z materials as titanium.Advances in knowledgeThe raw-data-based reconstruction method could reduce the beam hardening artifact compared with image-based reconstruction and increase the accuracy for the RED estimation in high-Z materials, such as titanium and iodinated contrast medium. 相似文献
60.
Cypripedium macranthos var. rebunense is an endangered plant endemic to Rebun Island, Japan. A proper understanding of genetic diversity is needed when conducting
conservation programs for rare and endangered species. We therefore examined the genetic diversity of C. macranthos var. rebunense using allozyme markers with a view to future conservation. Our study revealed that C. macranthos var. rebunense has relatively high genetic diversity (P was 0.62, n
a and n
e were 1.85 and 1.28 respectively, and H
o and H
e were 0.163 and 0.187, respectively) when compared with other plant taxa. The natural habitats of C. macranthos var. rebunense are geographically separated into northern and the southern populations. Disappearance of alleles and increase in homozygosity
expected as a result of the bottleneck effect were observed, particularly in the southern populations composed of a small
number of plants. As additional negative effects (inbreeding depression and further genetic drift) due to fragmentation are
predicted in these populations, the southern populations may show deterioration of genetic diversity in the near future. 相似文献