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901.
902.
903.
Synthesis of sulphatide-containing lipoproteins in rat brain 总被引:1,自引:1,他引:0
N Herschkowitz G M McKhann S Saxena E M Shooter R Herndon 《Journal of neurochemistry》1969,16(7):1049-1057
Abstract—
- 1 Puromycin inhibits [14C]leucine Hincorporation into brain proteins, but has no effect on the incorporation of [35S]sulphate into sulphatide. These effects of puromycin are observed not only with the proteins and sulphatide of whole brain, but also with the protein and sulphatide portion of water-soluble lipoprotein complexes.
- 2 Microsomes can be separated into three subfractions which differ chemically, morphologically and metabolically. Protein synthesis and sulphatide synthesis are located in different submicrosomal fractions.
- 3 The addition of water-soluble brain proteins to the incubation medium causes release of newly synthesized [35S]sulphatide and formation of soluble sulphatide protein complexes. One acceptor protein is identified as the lipoprotein previously shown to bind [35S]sulphatide in vivo (Herschkowitz , Mc Khann , Saxena and Shooter , 1968b).
- 4 These results suggest that protein and sulphatide synthesis can function independently and that association of newly synthesized lipid to preformed protein is possible.
904.
Summary The sieve plate pores of Salix caprea in preparations fixed in glutaraldehyde are normally found to be occupied by slime fibrils showing periodic banding such as occur in a number of other species. Arguments are put forward to suggest that the occurence of fibrils in this position is natural and not an artefact of preparation. The sieve tubes further possess prominent and persistent nucleoli showing a radiating structure of tubules. The endoplasmic reticulum often occurs in parietal stacks reminiscent of other species.This evidence is discussed in relation to the electroosmotic theory of translocation.This work formed part of that submitted for the degree of Ph. D. of the University of London by U. Mishra. 相似文献
905.
906.
Column chromatography of human small-intestinal maltase, isomaltase and invertase activities 总被引:5,自引:5,他引:0 下载免费PDF全文
1. The maltase, isomaltase and invertase (sucrase) activities of solubilized mucosal preparations from human jejunum and ileum were studied with column chromatography on anion-exchange (diethylaminoethyl- and triethylaminoethyl-)cellulose and Sephadex G-200 gel. 2. On ion-exchange cellulose columns both kinds of enzyme preparations yielded two major disaccharidase peaks. The first peak contained maltase Ia (=isomaltase) and maltase Ib (=invertase). The second peak contained maltase II and maltase III. 3. On Sephadex G-200 gel columns jejunal preparations yielded the corresponding peaks as on ion-exchange columns, but the peaks appeared in the reverse order in the effluent. The ileal preparation studied yielded a single peak on gel columns, containing all the activities studied and eluted with the `void volume'. 4. Precipitation with ethanol did not affect the behaviour of the enzymes during ion-exchange chromatography. When gel filtration was performed after ethanol precipitation of the enzymes, however, two peaks were obtained also with the ileal preparation, and subfractionation of the invertase was obtained with both kinds of preparations. 5. The second peak from ion-exchange chromatograms, containing maltase II and maltase III, on concentration was found to have very weak isomaltase activity, probably exerted by these enzymes as such. This activity accounts for only about 1% of the total isomaltase activity of the mucosa. 6. The results support the concept of the specificity of the human small-intestinal disaccharidases previously described after heat-inactivation experiments. The subfractionation of the invertase that under certain conditions is seen on Sephadex G-200 columns appears most likely to be an artifact. Consequently the nomenclature for the human maltose-, isomaltose- and sucrose-splitting enzymes proposed by another research group after gel-filtration chromatography studies should be abandoned. It seems more logical to keep the nomenclature based on heat inactivation [maltase Ia (=isomaltase), maltase Ib (=invertase or sucrase), maltase II and maltase III] until increased knowledge about the specificity and structure of these enzymes makes possible a more rational nomenclature. 相似文献
907.
908.
Comment on the use of cetylpyridinium chloride in the isolation of connective-tissue proteoglycan. 总被引:2,自引:2,他引:0 下载免费PDF全文
U Lindahl 《The Biochemical journal》1969,113(3):569-570
909.
910.
Zusammenfassung Bei einem Patienten mit multiplen Mißbildungen wurde eine Duplikation für die distale Hälfte vom kurzen Arm des Chromosoms 2 und eine Defizienz an einem C-Chromosom gefunden. In der Literatur sind vier Fälle mit ähnlicher Duplikation, jedoch jeweils einer klein n Defizienz am Chromosom 3 beschrieben worden. Ein Vergleich der klinischen Merkmale bei den fünf Patienten zeigt weitgehende Übereinstimmungen. Es wird gefolgert, daß die gleichartige Duplikation für das einheitliche klinische Bild der Patienten verantwortlich ist. Es wurden Chromosomenmessungen, Analysen der Replikationsmuster und Meioseuntersuchungen durchgeführt. Die Genloci für das Ss- und das Rh-System konnten von einer Lokalisierung auf dem duplizierten Segment ausgeschlossen werden.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献
2/C translocation in father and daughter: 46,XY t (2p-;Cp+) and 46,XX Cp+
Summary In a patient with multiple anomalies, a duplication comprising the distal half of the short arm of chromosome 2 and a small deficiency of a C-chromosome was found. Four other cases from the literature exhibit a similar duplication combined with a small deficiency each of chromosome 3. Comparison of the clinical pictures of the five patients revealed a conformity in the major features. It is concluded that the duplication is responsible for the uniform appearance of these patients. The studies performed include chromosome measurements, examination of replication patterns and meiosis. The gene loci for the Ss and Rh systems could be excluded from localization on the duplicated segment.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft. 相似文献