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971.
Divyangi Raghav Anupam Jyoti Arif Jamal Siddiqui Juhi Saxena 《Journal of applied microbiology》2022,133(2):287-310
There is an intricate network of relations between endophytic fungi and their hosts that affects the production of various bioactive compounds. Plant-associated endophytic fungi contain industrially important enzymes and have the potential to fulfil their rapid demand in the international market to boost business in technology. Being safe and metabolically active, they have replaced the usage of toxic and harmful chemicals and hold a credible application in biotransformation, bioremediation and industrial processes. Despite these, there are limited reports on fungal endophytes that can directly cater to the demand and supply of industrially stable enzymes. The underlying reasons include low endogenous production and secretion of enzymes from fungal endophytes which have raised concern for widely accepted applications. Hence, it is imperative to augment the biosynthetic and secretory potential of fungal endophytes. Modern state-of-the-art biotechnological technologies aiming at strain improvement using cell factory engineering as well as precise gene editing like Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and its Associated proteins (Cas) systems which can provide a boost in fungal endophyte enzyme production. Additionally, it is vital to characterize optimum conditions to grow one strain with multiple enzymes (OSME). The present review encompasses various plants-derived endophytic fungal enzymes and their applications in various sectors. Furthermore, we postulate the feasibility of new precision approaches with an aim for strain improvement and enhanced enzyme production. 相似文献
972.
973.
A statistical approach response surface methodology (RSM) was used to study the production of succinic acid from Bacteroides fragilis. The most influential parameters for succinic acid production obtained through one-at-a-time method were glucose, tryptone, sodium carbonate, inoculum size and incubation period. These resulted in the production of 5.4gL(-1) of succinic acid in 48h from B. fragilis under anaerobic conditions. Based on these results, a statistical method, face-centered central composite design (FCCCD) falling under RSM was employed for further enhancing the succinic acid production and to monitor the interactive effect of these parameters, which resulted in a more than 2-fold increase in yield (12.5gL(-1) in 24h). The analysis of variance (ANOVA) showed the adequacy of the model and the verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using conditions optimized through RSM, 20.0gL(-1) of succinic acid was obtained in 24h. This clearly indicated that the model stood valid even on large scale. Thus, the statistical optimization strategy led to an approximately 4-fold increase in the yield of succinic acid. This is the first report on the use of FCCCD to improve succinic acid production from B. fragilis. The present study provides useful information about the regulation of succinic acid synthesis through manipulation of various physiochemical parameters. 相似文献
974.
木豆CMS杂交种资源农艺性状及品质性状分析 总被引:1,自引:0,他引:1
对自ICR ISAT成套引进最新培育成功的早、中熟组木豆CMS杂交种各6份,进行了农艺性状及品质性状分析。结果表明:(1)早熟组的成株率高,育性恢复好;其中ICPH2363的成株率、单株荚数、百粒重等性状均表现较好。(2)中熟组中有2份杂交种在成株率、株高、分枝数等性状上远超对照品种;特别是ICPH3381的分枝数极显著多于对照品种。(3)早熟组杂交种的粗蛋白、无氮浸出物等指标普遍高于对照品种;特别是ICPH2364各品质性状指标均较高,干子粒平均单产也高于对照,作为精饲料、配合饲料开发利用潜力较大。木豆CMS杂交种资源农艺性状令人满意,在我国亚热带省区应具有较好的推广利用价值。 相似文献
975.
Bhatt J Subbaiah G Kambli S Shah B Patel M Saxena A Baliga A Nigam S Parekh H Yadav G 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2007,852(1-2):374-381
A high throughput and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of bisoprolol in human plasma using multiplexing technique (two HPLC units connected to one MS). Bisoprolol was extracted from human plasma using solid-phase extraction technique using metoprolol as internal standard. A Betabasic 8 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The mass transition ion-pair was followed as m/z 326.2-->116.1 for bisoprolol and m/z 268.2-->191.0 for metoprolol. The method involves a simple multiplexing, rapid solid-phase extraction, simple isocratic chromatography conditions and mass spectrometric detection which enable detection at sub-nanogram levels. The proposed method has been validated for a linear range of 0.5-70.0 ng/mL with correlation coefficient > or =0.9991. The precision and accuracy were within 10% for intra-HPLC runs and inter-HPLC runs. The overall recoveries for bisoprolol and metoprolol were 93.89% and 77.65%, respectively. Total MS run time was 0.90 min only. The developed method was applied for the determination of pharmacokinetic parameters of bisoprolol following a single oral administration of a 10mg bisoprolol tablet in 18 healthy male volunteers. 相似文献
976.
Geoffrey M. Reed Michael B. First Cary S. Kogan Steven E. Hyman Oye Gureje Wolfgang Gaebel Mario Maj Dan J. Stein Andreas Maercker Peter Tyrer Angelica Claudino Elena Garralda Luis Salvador‐Carulla Rajat Ray John B. Saunders Tarun Dua Vladimir Poznyak María Elena Medina‐Mora Kathleen M. Pike Jos L. Ayuso‐Mateos Shigenobu Kanba Jared W. Keeley Brigitte Khoury Valery N. Krasnov Maya Kulygina Anne M. Lovell Jair de Jesus Mari Toshimasa Maruta Chihiro Matsumoto Tahilia J. Rebello Michael C. Roberts Rebeca Robles Pratap Sharan Min Zhao Assen Jablensky Pichet Udomratn Afarin Rahimi‐Movaghar Per‐Anders Rydelius Sabine Bhrer‐Kohler Ann D. Watts Shekhar Saxena 《World psychiatry》2019,18(1):3-19
977.
Brijesh S Yadav Mayank Pokhriyal Barkha Ratta Ajay Kumar Meeta Saxena Bhaskar Sharma 《Bioinformation》2015,11(11):489-492
Viral diagnosis in Indian livestock using customized microarray chips is gaining momentum in recent years. Hence, it is possible to
design customized microarray chip for viruses infecting livestock in India. Customized microarray chips identified Bovine herpes
virus-1 (BHV-1), Canine Adeno Virus-1 (CAV-1), and Canine Parvo Virus-2 (CPV-2) in clinical samples. Microarray identified specific
probes were further confirmed using RT-PCR in all clinical and known samples. Therefore, the application of microarray chips
during viral disease outbreaks in Indian livestock is possible where conventional methods are unsuitable. It should be noted that
customized application requires a detailed cost efficiency calculation. 相似文献
978.
Rajendra K Baharia Rati Tandon Tanuj Sharma Manish K Suthar Sanchita Das Mohammad Imran Siddiqi Jitendra Kumar Saxena Shyam Sunder Anuradha Dube 《PLoS neglected tropical diseases》2015,9(3)
BackgroundThe development of a vaccine conferring long-lasting immunity remains a challenge against visceral leishmaniasis (VL). Immunoproteomic characterization of Leishmania donovani proteins led to the identification of a novel protein NAD+-dependent Silent Information regulatory-2 (SIR2 family or sirtuin) protein (LdSir2RP) as one of the potent immunostimulatory proteins. Proteins of the SIR2 family are characterized by a conserved catalytic domain that exerts unique NAD-dependent deacetylase activity. In the present study, an immunobiochemical characterization of LdSir2RP and further evaluation of its immunogenicity and prophylactic potential was done to assess for its possible involvement as a vaccine candidate against leishmaniasis.Conclusion/SignificanceThe immunobiochemical characterization strongly suggest the potential of rLdSir2RP as vaccine candidate against VL and supports the concept of its being effective T-cell stimulatory antigen. 相似文献
979.
980.
Deep Chatterjee Denis Kudlinzki Verena Linhard Krishna Saxena Ulrich Schieborr Santosh L. Gande Jan Philip Wurm Jens W?hnert Rupert Abele Vladimir V. Rogov Volker D?tsch Heinz D. Osiewacz Sridhar Sreeramulu Harald Schwalbe 《The Journal of biological chemistry》2015,290(26):16415-16430
Low levels of reactive oxygen species (ROS) act as important signaling molecules, but in excess they can damage biomolecules. ROS regulation is therefore of key importance. Several polyphenols in general and flavonoids in particular have the potential to generate hydroxyl radicals, the most hazardous among all ROS. However, the generation of a hydroxyl radical and subsequent ROS formation can be prevented by methylation of the hydroxyl group of the flavonoids. O-Methylation is performed by O-methyltransferases, members of the S-adenosyl-l-methionine (SAM)-dependent O-methyltransferase superfamily involved in the secondary metabolism of many species across all kingdoms. In the filamentous fungus Podospora anserina, a well established aging model, the O-methyltransferase (PaMTH1) was reported to accumulate in total and mitochondrial protein extracts during aging. In vitro functional studies revealed flavonoids and in particular myricetin as its potential substrate. The molecular architecture of PaMTH1 and the mechanism of the methyl transfer reaction remain unknown. Here, we report the crystal structures of PaMTH1 apoenzyme, PaMTH1-SAM (co-factor), and PaMTH1-S-adenosyl homocysteine (by-product) co-complexes refined to 2.0, 1.9, and 1.9 Å, respectively. PaMTH1 forms a tight dimer through swapping of the N termini. Each monomer adopts the Rossmann fold typical for many SAM-binding methyltransferases. Structural comparisons between different O-methyltransferases reveal a strikingly similar co-factor binding pocket but differences in the substrate binding pocket, indicating specific molecular determinants required for substrate selection. Furthermore, using NMR, mass spectrometry, and site-directed active site mutagenesis, we show that PaMTH1 catalyzes the transfer of the methyl group from SAM to one hydroxyl group of the myricetin in a cation-dependent manner. 相似文献