全文获取类型
收费全文 | 296篇 |
免费 | 12篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 12篇 |
2020年 | 2篇 |
2019年 | 5篇 |
2018年 | 11篇 |
2017年 | 10篇 |
2016年 | 13篇 |
2015年 | 19篇 |
2014年 | 11篇 |
2013年 | 27篇 |
2012年 | 27篇 |
2011年 | 29篇 |
2010年 | 11篇 |
2009年 | 15篇 |
2008年 | 13篇 |
2007年 | 13篇 |
2006年 | 21篇 |
2005年 | 14篇 |
2004年 | 12篇 |
2003年 | 6篇 |
2002年 | 7篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1995年 | 2篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1975年 | 2篇 |
1972年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有308条查询结果,搜索用时 46 毫秒
61.
A novel yeast species was isolated from leaf litter of Macropanax dispermus obtained from the Cibodas Botanical Garden, West Java, Indonesia. The two strains of the species displayed typical characteristics of the genus Citeromyces. Phylogenetic analysis based on the gene sequences of the D1/D2 domains of large subunit (LSU) rDNA, internal transcribed spacer (ITS) including 5.8S rDNA, mitochondrial small-subunit rRNA gene (MtSm), and translation elongation factor-1α (EF-1α) showed that the novel strains were clearly separated from the other four existing species of the genus Citeromyces. Therefore, the two strains were proposed to represent a novel species within the genus Citeromyces, for which the name Citeromyces cibodasensis is proposed; the type strain is NBRC 110244T (= CBS 14272T?=?InaCCY703T?=?AK 01). 相似文献
62.
Lucie Gueneau Richard J. Fish Hanan E. Shamseldin Norine Voisin Frédéric Tran Mau-Them Egle Preiksaitiene Glen R. Monroe Angeline Lai Audrey Putoux Fabienne Allias Qamariya Ambusaidi Laima Ambrozaityte Loreta Cimbalistienė Julien Delafontaine Nicolas Guex Mais Hashem Wesam Kurdi Saumya Shekhar Jamuar Alexandre Reymond 《American journal of human genetics》2018,102(1):116-132
63.
Parbati Chatterjee Manikkoth Chandroth Vyjayandi Narayan Ghorai Tushar Kanti Mukherjee 《Proceedings of the Zoological Society》2018,71(1):63-67
The holotype of Caliris keralensis (Vyjayandi et al. 2006) (Mantodea: Caliridinae) is reviewed along with another paratype female not listed by the authors. The male of this species is re-described in details and is assigned paratype male. Photographs of the specimens are given. Male genital complex is described for better understanding the species. 相似文献
64.
Hiroshi Kuriyama Dudi Sastraatmadja Yoko Igosaki Kaoru Watanabe Atit Kanti Takema Fukatsu 《Mycoscience》1997,38(4):441-445
Yeast strains with amylolytic activity were isolated from cassavatapé and its precursor,ragi. they were divided into two groups based on their characteristics: group 1, possessing high amylolytic activity and low ethanol
yield; and group 2, possessing low amylolytic activity and high ethanol yield. The major strains of the group 1 were identified
asEndomyces fibuliger, and those of group 2 were identified asPichia anomala. Based on 18S rDNA analysis, an isolate fromragi that had a high amylolytic activity was thought to be an undescribed species that was related to the basidiomycetous genera. 相似文献
65.
66.
Saumya K. Patel Vijay M. Khedkar Yogesh T. Jasrai Himanshu A. Pandya Linz-Buoy George 《Journal of biomolecular structure & dynamics》2016,34(2):290-303
Phytochemicals of Catharanthus roseus Linn. and Tylophora indica have been known for their inhibition of malarial parasite, Plasmodium falciparum in cell culture. Resistance to chloroquine (CQ), a widely used antimalarial drug, is due to the CQ resistance transporter (CRT) system. The present study deals with computational modeling of Plasmodium falciparum chloroquine resistance transporter (PfCRT) protein and development of charged environment to mimic a condition of resistance. The model of PfCRT was developed using Protein homology/analogy engine (PHYRE ver 0.2) and was validated based on the results obtained using PSI-PRED. Subsequently, molecular interactions of selected phytochemicals extracted from C. roseus Linn. and T. indica were studied using multiple-iterated genetic algorithm-based docking protocol in order to investigate the translocation of these legends across the PfCRT protein. Further, molecular dynamics studies exhibiting interaction energy estimates of these compounds within the active site of the protein showed that compounds are more selective toward PfCRT. Clusters of conformations with the free energy of binding were estimated which clearly demonstrated the potential channel and by this means the translocation across the PfCRT is anticipated. 相似文献
67.
Summary A study has been made of the mineral requirements of Streptomyces kanamyceticus KCC S-0433 for production of glucose isomerase. The optimal concentrations of MgSO4 and K2HPO4 for enzyme production are 0.07% and 0.05%, respectively. The elements Fe, Mn and Zn are required at levels of 10, 3 and 3 mg/l, respectively. Cu, Co and Ca have inhibitory effects on the production of the enzyme. 相似文献
68.
We present the theoretical foundations of a general principle to infer structure ensembles of flexible biomolecules from spatially and temporally averaged data obtained in biophysical experiments. The central idea is to compute the Kullback-Leibler optimal modification of a given prior distribution with respect to the experimental data and its uncertainty. This principle generalizes the successful inferential structure determination method and recently proposed maximum entropy methods. Tractability of the protocol is demonstrated through the analysis of simulated nuclear magnetic resonance spectroscopy data of a small peptide. 相似文献
69.
Background
Bluetongue virus (BTV) particles consist of seven structural proteins that are organized into two capsids. In addition, BTV also encodes three non-structural (NS) proteins of which protein 2 (NS2) is the RNA binding protein and is also the major component of virus encoded inclusion bodies (VIBs), which are believed to be virus assembly sites. To investigate the contribution of NS2 in virus replication and assembly we have constructed inducible mammalian cell lines expressing full-length NS2. In addition, truncated NS2 fragments were also generated in an attempt to create dominant negative mutants for NS2 function. 相似文献70.
Malignant mesothelioma is an aggressive cancer of the pleura that is causally related to exposure to asbestos fibres. The kallikrein serine proteases [tissue (hK1) and plasma (hKB1) kallikreins, and kallikrein-related peptidases (KRP/hK2-15)] and the mitogenic kinin peptides may have a role in tumourigenesis. However, it is not known whether hK1, hKB1, KRP/hK proteins or kinin receptors are expressed in pleural mesotheliomas. The expression of hK1, hKB1, KRP/hK2, 5, 6, 7, 8 and 9, and kinin B(1) and B(2) receptors was assessed in archived selected normal tissue and mesothelioma tumour sections by immunoperoxidase and immunofluorescence labelling. hK1, hKB1 and kinin B(1) and B(2) receptors were expressed in malignant cells of the epithelioid and sarcomatoid components of biphasic mesothelioma tumour cells. The percentage of cells with cytoplasmic and nuclear labelling and the intensity of labelling were similar for hK1, hKB1 and the kinin receptors. KRP/hK2, 6, 8 and 9 were also expressed in the cytoplasm and nuclei of mesothelioma cells, whereas KRP/hK5 and hK7 showed predominantly cytoplasmic localisation. This is a first report, but further studies are required to determine whether these proteins have a functional role in the pathogenesis of mesothelioma and/or may be potential biomarkers for pleural mesothelioma. 相似文献