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31.
Maung Maung Theint San Thwe Thidalay Myat Myat Zaw Khin Shimada Tomofumi Bawm Saw Kobayashi Motoko Saing Khin Maung Katakura Ken Arai Satoru Suzuki Hitoshi 《Journal of Mammalian Evolution》2021,28(2):497-509
Journal of Mammalian Evolution - We addressed the spatiotemporal characteristics of four commensal rodent species occurring in Myanmar in comparison with other areas of the Indo-Malayan region. We... 相似文献
32.
Hiroki Kumamoto Hiroyuki Hayakawa Hiromichi Tanaka Satoru Shindoh Keisuke Kato Tadashi Miyasaka 《Nucleosides, nucleotides & nucleic acids》2013,32(1-3):15-27
Abstract Based on the recently developed lithiation-mediated stannyl migration of 6-chloropurine derivatives, 2-iodocordycepin was prepared from cordycepin. The reaction of this compound with terminal alkynes was carried out to synthesize a series of 2-alkynyl derivatives. The vasodilating effect of these compounds was evaluated. 相似文献
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Andreia Cruz Dário Areias Ana Duarte António Correia Satoru Suzuki Sónia Mendo 《Antonie van Leeuwenhoek》2013,104(3):385-396
Aeromonas molluscorum Av27 is an estuarine bacterium highly resistant to tributyltin (TBT). Also, the strain is able to degrade TBT into the less toxic compounds dibutyltin and monobutyltin. Therefore, this bacterium has potential to be employed in bioremediation processes. In this context, defining its biological safety is crucial. With that purpose a number of intrinsic characteristics, usually present/associated with virulent strains, were investigated. Few virulence factors were detected in strain Av27. For instance, a DNase gene is present, but it is not apparently expressed in vitro. Motility, adherence factor and phospholipase activity were also detected. Additionally, cytotoxicity to Vero cells was negative. Resistance to penicillin (10 μg ml?1), amoxicillin/clavulanic acid (30 μg ml?1) and cephalothin (30 μg ml?1) and also to the vibriostatic agent O/129 was observed. Five plasmids (4, 7, 10, 100 kb and one greater than 100 kb) were identified. No Class I and II integrons were detected. Study of the optimal growth conditions showed that Av27 easily adapts to different environmental conditions. Overall, the results suggest that A. molluscorum Av27 can be considered safe to use to bioremediate TBT in contaminated environments. 相似文献
36.
Keiji Saito Akira Nakao Tsuyoshi Shinozuka Kousei Shimada Satoshi Matsui Kiyoshi Oizumi Kazuki Yano Keiko Ohata Daisuke Nakai Yoko Nagai Satoru Naito 《Bioorganic & medicinal chemistry》2013,21(7):1628-1642
A cell-based assay was performed for the discovery of novel bone anabolic agents. Alkaline phosphatase (ALPase) activity of ST2 cells was utilized as an indicator of osteoblastic differentiation, and thienopyridine derivative 1 was identified as a hit compound. 3-Aminothieno[2,3-b]pyridine-2-carboxamide was confirmed to be a necessary core structure for the enhancement of ALPase activity, and then optimization of the C4-substituent on the thienopyridine ring was carried out. Introduction of cyclic amino groups to the C4-position of the thienopyridine ring improved the activity. Especially, N-phenyl-homopiperazine derivatives were found to be strong enhancers of ALPase among this new series. Furthermore, 3-amino-4-(4-phenyl-1,4-diazepan-1-yl)thieno[2,3-b]pyridine-2-carboxamide (15k) was orally administered to ovariectomized (OVX) rats over 6 weeks for evaluating the effects on areal bone mineral density (aBMD), and statistically significant improvements in aBMD were observed from the dosage of 10 mg/kg/day. 相似文献
37.
Satoru Oka 《Bioscience, biotechnology, and biochemistry》2013,77(8):515-519
The adsorption of phenols and esters of acid antiseptics by the bacterial cell (Escherichia coli and Staphylococcus aureus) was investigated in relatoin to their toxic effect, and it has been observed that the definite quantity of antiseptics must be adsorbed on the solid phase of the bacterial cell in order to give the definite toxic effect, and the toxic effect is independent of the quantity dissolved in the inner cell fluid or in the lipid phase of the cell. The result shows that the toxic effect of these antiseptics on either bacteria and yeast, is exclusively limited by the adsorbed quantity.The adsorbed quantity required for the definite toxic effect was nearly the same as that previously observed in the case of the yeast, and the mechanism of the toxic action of these antiseptics was assumed to be same with each other in any case of microbes. 相似文献
38.
Satoru Oka 《Bioscience, biotechnology, and biochemistry》2013,77(8):520-525
The bacterial growth is inhibited by nitrofurane compounds, although the yeast growth is hardly affected. In relation to the selective toxicity of nitrofuranes for bacteria, the interaction between microbes (Escherichia coli, Staphylococcus aureus and bakers– yeast) and nitrofurane compounds (5-nitro-2-furfural semicarbazone and 5-nitro-2-furylacryl amide) was examined.Apparently, in the bacterial suspension containing energy substrate, nitrofuranes are continuously reduced to corresponding aminofuranes, respectively. The velocity of the bacterial reduction at the growth inhibiting condition was evaluated as great as above 30 per cent of the limit of supplying velocity of coenzymes in the cell, the reduction velocity of such value is enough to inhibit the bacterial growth, because the electron transfer in the cell metabolism is disordered.On the other hand, in the yeast suspension, the reduction velocity was negligibly small. The difference of the reduction ability between bacteria and yeast was seemingly owing to the fact that the permeability of the nitrofuranes differs by the kind of microbe so that it was concluded that the antimicrobial effect of nitrofuranes is limited by the permeability for the microbe cell. 相似文献
39.
Tsutomu Yoshida Shoko Shinoda Tsuneya Matsumoto Satoru Watarai 《Bioscience, biotechnology, and biochemistry》2013,77(12):3093-3095
A strain of Alcaligenes isolated from soil was a good producer of β-glucuronidase, and the enzyme was purified from the cell-free extract by sequential column chromatography on DEAE-Toyopearl, Toyopearl HW-55F, and Phenyl-Sepharose CL-4B. By these procedures, two β-glucuronidases designated as β-glucuronidases I and II were purified 240- and 508-fold, respectively. β-Glucuronidase I, with a molecular weight of 75,000, had an optimum pH at 7.5 and the enzyme II, with a molecular weight of 300,000, had maximum activity at pH 6.0. Both enzymes were strongly inhibited by saccharo-1,4-lactone, glucaro-δ-lactam, p-chloromercuribenzoate, Hg2+, and N-bromosuccinimide. β-Glucuronidase I was active toward estrogen-3-β-glucuronides and inert toward β-glucuronide conjugates of menthol, estrogen-17β-, estrogen-16α-, androsterone-3α-, testosterone-17β-, cortisol-17α-. β-Glucuronidase II hydrolyzed all of these substrates. β-Glucuronidase I was inhibited by phenolphthalein and its glucuronide. 相似文献
40.
Yoshiki Koriyama Yusuke Takagi Kenzo Chiba Matsumi Yamazaki Kayo Sugitani Kunizo Arai Hirokazu Suzuki Satoru Kato 《PloS one》2013,8(8)
Like other CNS neurons, mature retinal ganglion cells (RGCs) are unable to regenerate their axons after nerve injury due to a diminished intrinsic regenerative capacity. One of the reasons why they lose the capacity for axon regeneration seems to be associated with a dramatic shift in RGCs’ program of gene expression by epigenetic modulation. We recently reported that (1R)-isoPropyloxygenipin (IPRG001), a genipin derivative, has both neuroprotective and neurite outgrowth activities in murine RGC-5 retinal precursor cells. These effects were both mediated by nitric oxide (NO)/S-nitrosylation signaling. Neuritogenic activity was mediated by S-nitrosylation of histone deacetylase-2 (HDAC2), which subsequently induced retinoic acid receptor β (RARβ) expression via chromatin remodeling in vitro. RARβ plays important roles of neural growth and differentiation in development. However, the role of RARβ expression during adult rat optic nerve regeneration is not clear. In the present study, we extended this hypothesis to examine optic nerve regeneration by IPRG001 in adult rat RGCs in vivo. We found a correlation between RARβ expression and neurite outgrowth with age in the developing rat retina. Moreover, we found that IPRG001 significantly induced RARβ expression in adult rat RGCs through the S-nitrosylation of HDAC2 processing mechanism. Concomitant with RARβ expression, adult rat RGCs displayed a regenerative capacity for optic axons in vivo by IPRG001 treatment. These neuritogenic effects of IPRG001 were specifically suppressed by siRNA for RARβ. Thus, the dual neuroprotective and neuritogenic actions of genipin via S-nitrosylation might offer a powerful therapeutic tool for the treatment of RGC degenerative disorders. 相似文献