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371.
Chiranjit Maity Saptadip Samanta Suman K. Halder Pradeep K. Das Mohapatra Bikas R. Pati Malabendu Jana Keshab C. Mondal 《Biotechnology and Bioprocess Engineering》2011,16(2):312-319
The aim of this study was to produce two isozymes of α-amylase by immobilization of a newly isolated soil bacterium. The bacterium
was identified as Bacillus thuringiensis CKB19 on the basis of its 16S rRNA profile. Enzyme production by free cells increased linearly with cell growth up to 34
h in starch containing enriched liquid media. The active bacterial cells were immobilized in Caalginate beads, and operational
stability of the entrapped cell was optimized for amylase production. Enzyme production was optimal at an alginate concentration
of 2 g% (w/v), calcium chloride concentration of 1 M, and with 300 beads (each bead contained 2 × 107 cells)/250 mL flask. Amylase production by the immobilized cells was about 3 times higher than free cell fermentation after
34 h of incubation. It was observed that the immobilized bacterium secreted two different amylases (Am-I and Am-II) into the
culture fluid. The molecular masses of Am-I and Am-II were 59.6 and 44.7 kd, respectively, and showed optimum activity at
pH 5.0 and 9.0. Both amylases showed optimum activity at 40°C and were stable at the same temperature, with losses of only
10 and 20% (for Am I and Am II, respectively) of their original activities after 24 h of incubation. Further, both amylases
were salt tolerant (up to 4 M NaCl) and hydrolyzed raw starchy foods into glucose. All these characteristics make this enzyme
mixture suitable for use as a digestive aid and for the improvement of digestibility of animal feed ingredients. 相似文献
372.
The bull seminal plasma peptides α andβ have been examined for their biological properties. While both the peptides were able to inhibit the human chorionic gonadotropin-dependent
uterine response in the mouse, α alone exhibits the property of suppressing post-castrational rise in gonadotropin in appropriate
animal models. This suggests that the peptideβ must be acting directly on the ovary to suppress estrogen production and, consequently, the uterine weight increase. Such
a possibility was confirmed when α andβ were examined by the coupled bioassay which is capable of discriminating between pituitary feedback factors and those acting
directly on the gonad. In a test system designed to examine chronic effects, both α andβ showed evidence of acting directly on the ovary to inhibit human menopausal gonadotropin-induced estrogen production. Such
a direct action could not be correlated with the relative potencies of these peptides when examined for their follicle stimulating
hormone-receptor binding inhibitor and lutinizing hormone-receptor binding inhibitor activities. 相似文献
373.
374.
375.
Anuradha Upadhyay T. Mohapatra R. A. Pai R. P. Sharma 《Journal of plant biochemistry and biotechnology.》1996,5(1):17-22
In Brassica juncea, segregation of 44 RFLP markers generated by homologous genomic DNA clones as probes was studied in a F2 population obtained from an intervarietal cross. Linkage relationship among the markers was established using computer package ‘MAPMAKER’. Twenty five of the markers could be arranged in nine linkage groups, covering a total of 243.3 cM. Marker BJG357c showed tight linkage (3.9 cM) with yellow seed coat colour locus (r 1). Based on single factor analysis of variance,17 significant marker-trait associations could be established in respect of six quantitative traits viz. days to flowering, plant height, number of primary branches, secondary branches per primary branch, siliquea per secondary branch, and seeds per siliqua. The proportion of phenotypic variation explained by individual marker-trait association ranged from 3.0% to 33.2%. The putative gene action at majority of the marked genomic regions was found to be partial dominance to dominance. 相似文献