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111.
Siddique M Gernhard S von Koskull-Döring P Vierling E Scharf KD 《Cell stress & chaperones》2008,13(2):183-197
The small heat shock proteins (sHsps), which are ubiquitous stress proteins proposed to act as chaperones, are encoded by
an unusually complex gene family in plants. Plant sHsps are classified into different subfamilies according to amino acid
sequence similarity and localization to distinct subcellular compartments. In the whole Arabidopsis thaliana genome, 19 genes were annotated to encode sHsps, of which 14 belong to previously defined plant sHsp families. In this paper,
we report studies of the five additional sHsp genes in A. thaliana, which can now be shown to represent evolutionarily distinct sHsp subfamilies also found in other plant species. While two
of these five sHsps show expression patterns typical of the other 14 genes, three have unusual tissue specific and developmental
profiles and do not respond to heat induction. Analysis of intracellular targeting indicates that one sHsp represents a new
class of mitochondrion-targeted sHsps, while the others are cytosolic/nuclear, some of which may cooperate with other sHsps
in formation of heat stress granules. Three of the five new proteins were purified and tested for chaperone activity in vitro.
Altogether, these studies complete our basic understanding of the sHsp chaperone family in plants.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
112.
Jasmin Kirchner Emese Vissi Sascha Gross Balazs Szoor Andrey Rudenko Luke Alphey Helen White-Cooper 《BMC molecular biology》2008,9(1):36
Background
Protein phosphatase 1 (PP1) is involved in diverse cellular processes, and is targeted to substrates via interaction with many different protein binding partners. PP1 catalytic subunits (PP1c) fall into PP1α and PP1β subfamilies based on sequence analysis, however very few PP1c binding proteins have been demonstrated to discriminate between PP1α and PP1β. 相似文献113.
Díaz-Carballo D Malak S Bardenheuer W Freistuehler M Peter Reusch H 《Bioorganic & medicinal chemistry》2008,16(22):9635-9643
Increasing efforts are directed toward finding applications for natural products and their derivatives in the treatment of human diseases. Among such products, propolis, a resinous substance produced by honey bees from various plant sources, has been found to be a promising source of potential therapeutics. In the present work, we aimed at studying the perspective of Cuban propolis as a source of possible anti-cancer agents. We found an anti-metastatic effect in mice and considerable cytotoxicity without cross-resistance in both wild-type and chemoresistant human tumor cell lines. Plukenetione A--identified for the first time in Cuban propolis--induced G0/G1 arrest and DNA fragmentation in colon carcinoma cells. Furthermore, the activities of both topoisomerase I and DNA polymerase were inhibited, while the expression of topoisomerase II-beta, EGF receptor, and multidrug resistance-related protein genes was found repressed. We assume that plukenetione A contributes to the anti-tumoral effect of Cuban propolis mainly by targeting topoisomerase I as well as DNA polymerase. 相似文献
114.
Myxozoan parasitism in waterfowl 总被引:2,自引:0,他引:2
Bartholomew JL Atkinson SD Hallett SL Lowenstine LJ Garner MM Gardiner CH Rideout BA Keel MK Brown JD 《International journal for parasitology》2008,38(10):1199-1207
Myxozoans are spore-forming, metazoan parasites common in cold-blooded aquatic vertebrates, especially fishes, with alternate life cycle stages developing in invertebrates. We report nine cases of infection in free-flying native and captive exotic ducks (Anseriformes: Anatidae) from locations across the United States and describe the first myxozoan in birds, Myxidium anatidum n. sp. We found developmental stages and mature spores in the bile ducts of a Pekin duck (domesticated Anas platyrhynchos). Spores are lens-shaped in sutural view, slightly sigmoidal in valvular view, with two polar capsules, and each valve cell has 14-16 longitudinal surface ridges. Spore dimensions are 23.1 microm x 10.8 microm x 11.2 microm. Phylogenetic analysis of the ssrRNA gene revealed closest affinity with Myxidium species described from chelonids (tortoises). Our novel finding broadens the definition of the Myxozoa to include birds as hosts and has implications for understanding myxozoan evolution, and mechanisms of geographical and host range extension. The number of infection records indicates this is not an incidental occurrence, and the detection of such widely dispersed cases suggests more myxozoans in birds will be encountered with increased surveillance of these hosts for pathogens. 相似文献
115.
116.
Birgit Ziegenhagen Silke Gneuss Georg Rathmacher Ilona Leyer Ronald Bialozyt Berthold Heinze Sascha Liepelt 《Conservation Genetics》2008,9(2):373-379
It is known that various poplar species and cultivated poplar hybrids have the potential to interbreed and produce fertile
offspring. Conservation strategies for the genetic resources of the endangered Eurasian black poplar (Populus nigra L.) thus rely on a monitoring which enables the identification and verification of the pure species status. At the same time,
the risk of hybrid dispersal and introgressive gene flow has to be estimated. In the present study a combination of two molecular
markers, one from chloroplast DNA and the other from nuclear DNA, was applied to evaluate a large P. nigra population on the Elbe River. Hybrid clones of P. × canadensis are scattered within this population and also occur as plantations in the surrounding landscape. By means of the DNA markers
the taxonomic status of 208 adult trees in the population and 140 young poplars along the riverbank was monitored. From the
analysed young poplars, almost 20 percent were found to exhibit at least one of the two P.
deltoides or P. × canadensis diagnostic alleles or genotypes, respectively. Possible vegetative spreads of F1 hybrids and precedent mating scenarios are
discussed. Most interestingly we found clear evidence for a small number of backcross hybrids where P. × canadensis acted as pollen donor. This case had long been debated and thought to be less probable, so far. 相似文献
117.
Claudine Dherin Emeric Gueneau Mathilde Francin Marcela Nunez Simona Miron Sascha Emilie Liberti Lene Juel Rasmussen Sophie Zinn-Justin Bernard Gilquin Jean-Baptiste Charbonnier Serge Boiteux 《Molecular and cellular biology》2009,29(3):907-918
Mlh1 is an essential factor of mismatch repair (MMR) and meiotic recombination. It interacts through its C-terminal region with MutL homologs and proteins involved in DNA repair and replication. In this study, we identified the site of yeast Mlh1 critical for the interaction with Exo1, Ntg2, and Sgs1 proteins, designated as site S2 by reference to the Mlh1/Pms1 heterodimerization site S1. We show that site S2 is also involved in the interaction between human MLH1 and EXO1 or BLM. Binding at this site involves a common motif on Mlh1 partners that we called the MIP-box for the Mlh1 interacting protein box. Direct and specific interactions between yeast Mlh1 and peptides derived from Exo1, Ntg2, and Sgs1 and between human MLH1 and peptide derived from EXO1 and BLM were measured with Kd values ranging from 8.1 to 17.4 μM. In Saccharomyces cerevisiae, a mutant of Mlh1 targeted at site S2 (Mlh1-E682A) behaves as a hypomorphic form of Exo1. The site S2 in Mlh1 mediates Exo1 recruitment in order to optimize MMR-dependent mutation avoidance. Given the conservation of Mlh1 and Exo1 interaction, it may readily impact Mlh1-dependent functions such as cancer prevention in higher eukaryotes. 相似文献
118.
Kálmán Molnár Csaba Székely Sascha L. Hallett Stephen D. Atkinson 《Systematic parasitology》2009,72(1):71-79
Myxobolus rotundus Nemeczek, 1911 is a common and specific parasite of the common bream Abramis brama (L.). Small, round or ellipsoidal shaped plasmodia of this species develop in the gill and exhibit strong histotropism to
the secondary gill lamellae with plasmodial development in their capillary network. M. rotundus is frequently found in mixed infection with M. bramae Reuss, 1906, a parasite of the afferent arteries of gill filaments. The round spores of M. rotundus resemble several other Myxobolus spp., but can be distinguished from these by their small subunit ribosomal RNA gene sequence (GenBank accession no. EU710583),
which also differs from the sequence for ‘M. rotundus’ from the skin of Chinese goldfish Carassius auratus auratus (L.), which we suggest has been misidentified. The SSU rRNA gene sequence of M. rotundus myxospores from bream corresponded to Triactinomyxon type 4 actinospores (AY495707) isolated from Tubifex tubifex (Müller) by Hallett et al. (2005), and we infer from this that these are alternate life stages. 相似文献
119.
Moradi Ahmad B. Conesa Héctor M. Robinson Brett Lehmann Eberhard Kuehne Guido Kaestner Anders Oswald Sascha Schulin Rainer 《Plant and Soil》2009,320(1-2):243-254
Chronic atmospheric nitrogen deposition affects the cycling of carbon (C) and nitrogen (N) in forest ecosystems, and thereby alters the stable C isotopic abundance of plant and soil. Three successional stages, disturbed, rehabilitated and mature forests were studied for their responses to different nitrogen input levels. N-addition manipulative experiments were conducted at low, medium and high N levels. To study the responses of C cycling to N addition, the C concentration and 13C natural abundances for leaf, litter and soil were measured. Labile organic carbon fractions in mineral soils were measured to quantify the dynamics of soil organic C (SOC). Results showed that three-year continuous N addition did not significantly increase foliar C and N concentration, but decreased C/N ratio and enriched 13C in N-rich forests. In addition, N addition significantly decreased microbial biomass C, and increased water soluble organic C in surface soils of N-rich forests. This study suggests that N addition enhances the water consumption per unit C assimilation of dominant plant species, restricts SOC turnover in N-poor forests at early and medium successional stages (thus favored SOC sequestration), and vice versa for N-rich mature forests. 相似文献
120.
Barrero C Royo J Grijota-Martinez C Faye C Paul W Sanz S Steinbiss HH Hueros G 《Planta》2009,229(2):235-247