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51.
Masci A Mastronicola D Arese M Piane M De Amicis A Blanck TJ Chessa L Sarti P 《Biochimica et biophysica acta》2008,1777(1):66-73
Ataxia Telangiectasia (AT) patients are particularly sensitive to oxidative-nitrosative stress. Nitric oxide (NO) controls mitochondrial respiration via the reversible inhibition of complex IV. The mitochondrial response to NO of AT lymphoblastoid cells was investigated. Cells isolated from three patients and three intrafamilial healthy controls were selected showing within each group a normal diploid karyotype and homogeneous telomere length. Different complex IV NO-inhibition patterns were induced by varying the electron flux through the respiratory chain, using exogenous cell membrane permeable electron donors. Under conditions of high electron flux the mitochondrial NO inhibition of respiration was greater in AT than in control cells (P< or =0.05). This property appears peculiar to AT, and correlates well to the higher concentration of cytochrome c detected in the AT cells. This finding is discussed on the basis of the proposed mechanism of reaction of NO with complex IV. It is suggested that the peculiar response of AT mitochondria to NO stress may be relevant to the mitochondrial metabolism of AT patients. 相似文献
52.
Recovery of antibodies with Protein A affinity chromatography columns has become the standard for the biotechnology industry. Membrane affinity chromatography has not yet experienced extensive application due to the lower capacity of membrane supports compared to chromatographic beads. In this work, new affinity membranes endowed with an interesting binding capacity for human IgG are studied in view of their application in the capturing step of a monoclonal antibody production process. The membranes have been extensively tested with pure IgG solutions and with a cell culture supernatant containing IgG1. The effects of feed flow rate and IgG concentration on the separation performances have been studied in detail, considering in particular binding capacity, selectivity and recovery. These new high capacity affinity membranes appear good candidates to avoid the throughput limitations and other well-known drawbacks of traditional bead-based chromatographic columns. 相似文献
53.
E Forte A Urbani M Saraste P Sarti M Brunori A Giuffrè 《European journal of biochemistry》2001,268(24):6486-6491
The cytochrome cbb3 is an isoenzyme in the family of cytochrome c oxidases. This protein purified from Pseudomonas stutzeri displays a cyanide-sensitive nitric oxide reductase activity (Vmax=100+/-9 mol NO x mol cbb3(-1) x min(-1) and Km=12+/-2.5 microm), which is lost upon denaturation. This enzyme is only partially reduced by ascorbate, and readily re-oxidized by NO under anaerobic conditions at a rate consistent with the turnover number for NO consumption. As shown by transient spectroscopy experiments and singular value decomposition (SVD) analysis, these results suggest that the cbb3-type cytochromes, sharing structural features with bacterial nitric oxide reductases, are the enzymes retaining the highest NO reductase activity within the heme-copper oxidase superfamily. 相似文献
54.
Francesco Malatesta Giovanni Antonin Paolo Sarti Beatrice Vallone Maurizio Brunori 《Biometals》1990,3(2):118-121
Summary The kinetics of electron entry in beef heart cytochromec oxidase have been studied by stopped-flow spectroscopy following chemical modification of the CuA site with mercurials. In this derivative CuA is no longer reducible by cytochrome c while cytochromea may accept electrons from the latter with rates comparable to the native enzyme. The results indicate that CuA is not the exclusive electron entry site in cytochromec oxidase. 相似文献
55.
Differential effects of c-myb and c-fes antisense oligodeoxynucleotides on granulocytic differentiation of human myeloid leukemia HL60 cells 总被引:2,自引:0,他引:2
S Ferrari A Donelli R Manfredini M Sarti R Roncaglia E Tagliafico E Rossi G Torelli U Torelli 《Cell growth & differentiation》1990,1(11):543-548
To gain some insight into the role of c-myb and c-fes in myeloid differentiation, the authors have analyzed the ability of HL60 cells to differentiate in response to several different inducers after inhibition of c-myb and c-fes function. This function has been inhibited almost completely by using deoxynucleotides complementary to two 18-nucleotide sequences of c-myb and c-fes encoding mRNA. After 5 days in culture, in several separate experiments with different oligomer preparations, more than 90% growth inhibition was observed in c-myb antisense-treated HL60 cells. At this time, independent of the differentiation inducer used, c-myb antisense-treated HL60 cells differentiate only along the monocytic pathway, whereas in sense oligomer-treated cultures, retinoic acid and dimethyl sulfoxide induced granulocytic differentiation. No perturbation of the HL60 cell growth was observed after 5 days of treatment with antisense c-fes oligomer. However, induction to granulocytic differentiation by retinoic acid and dimethyl sulfoxide resulted in progressive cell death, whereas monocytic differentiation by other differentiation inducers was only marginally affected. These results suggest that granulocytic, unlike monocytic, differentiation requires c-myb-conditioned proliferation and the activity of the protein encoded by c-fes. 相似文献
56.
57.
Jo?o B. Vicente Henrique G. Cola?o Marisa I. S. Mendes Paolo Sarti Paula Leandro Alessandro Giuffrè 《The Journal of biological chemistry》2014,289(12):8579-8587
The hexa-coordinate heme in the H2S-generating human enzyme cystathionine β-synthase (CBS) acts as a redox-sensitive regulator that impairs CBS activity upon binding of NO• or CO at the reduced iron. Despite the proposed physiological relevance of this inhibitory mechanism, unlike CO, NO• was reported to bind at the CBS heme with very low affinity (Kd = 30–281 μm). This discrepancy was herein reconciled by investigating the NO• reactivity of recombinant human CBS by static and stopped-flow UV-visible absorption spectroscopy. We found that NO• binds tightly to the ferrous CBS heme, with an apparent Kd ≤0.23 μm. In line with this result, at 25 °C, NO• binds quickly to CBS (kon ∼ 8 × 103
m−1 s−1) and dissociates slowly from the enzyme (koff ∼ 0.003 s−1). The observed rate constants for NO• binding were found to be linearly dependent on [NO•] up to ∼ 800 μm NO•, and >100-fold higher than those measured for CO, indicating that the reaction is not limited by the slow dissociation of Cys-52 from the heme iron, as reported for CO. For the first time the heme of human CBS is reported to bind NO• quickly and tightly, providing a mechanistic basis for the in vivo regulation of the enzyme by NO•. The novel findings reported here shed new light on CBS regulation by NO• and its possible (patho)physiological relevance, enforcing the growing evidence for an interplay among the gasotransmitters NO•, CO, and H2S in cell signaling. 相似文献
58.
Stefania Romeo Maurizio Sarti Maria Rosaria Scarfì Luigi Zeni 《The Journal of membrane biology》2010,236(1):55-60
Intense nanosecond pulsed electric fields (nsPEFs) have been shown to induce, on intracellular structures, interesting effects
dependent on electrical exposure conditions (pulse length and amplitude, repetition frequency and number of pulses), which
are known in the literature as “bioelectrical effects” (Schoenbach et al., IEEE Trans Plasma Sci 30:293–300, 2002). In particular, pulses with a shorter width than the plasma membrane charging time constant (about 100 ns for mammalian
cells) can penetrate the cell and trigger effects such as permeabilization of intracellular membranes, release of Ca2+ and apoptosis induction. Moreover, the observed effects have led to exploration of medical applications, like the treatment
of melanoma tumors (Nuccitelli et al., Biochem Biophys Res Commun 343:351–360, 2006). Pulsed electric fields allowing such effects usually range from several tens to a few hundred nanoseconds in duration and
from a few to several tens of megavolts per meter in amplitude (Schoenbach et al., IEEE Trans Diel Elec Insul 14:1088–1109,
2007); however, the biological effects of subnanosecond pulses have been also investigated (Schoenbach et al., IEEE Trans Plasma
Sci 36:414–422, 2008). The use of such a large variety of pulse parameters suggests that highly flexible pulse-generating systems, able to deliver
wide ranges of pulse durations and amplitudes, are strongly required in order to explore effects and applications related
to different exposure conditions. The Blumlein pulse-forming network is an often-employed circuit topology for the generation
of high-voltage electric pulses with fixed pulse duration. An innovative modification to the Blumlein circuit has been recently
devised which allows generation of pulses with variable amplitude, duration and polarity. Two different modified Blumlein
pulse-generating systems are presented in this article, the first based on a coaxial cable configuration, matching microscopic
slides as a pulse-delivery system, and the other based on microstrip transmission lines and designed to match cuvettes for
the exposure of cell suspensions. 相似文献
59.
60.
The cytochrome bd ubiquinol oxidase from Escherichia coli couples the exergonic two-electron oxidation of ubiquinol and four-electron reduction of O(2) to 2H(2)O to proton motive force generation by transmembrane charge separation. The oxidase contains two b-type hemes (b(558) and b(595)) and one heme d, where O(2) is captured and converted to water through sequential formation of a few intermediates. The spectral features of the isolated cytochrome bd at steady-state have been examined by stopped-flow multiwavelength absorption spectroscopy. Under turnover conditions, sustained by O(2) and dithiothreitol (DTT)-reduced ubiquinone, the ferryl and oxy-ferrous species are the mostly populated catalytic intermediates, with a residual minor fraction of the enzyme containing ferric heme d and possibly one electron on heme b(558). These findings are unprecedented and differ from those obtained with mammalian cytochrome c oxidase, in which the oxygen intermediates were not found to be populated at detectable levels under similar conditions [M.G. Mason, P. Nicholls, C.E. Cooper, The steady-state mechanism of cytochrome c oxidase: redox interactions between metal centres, Biochem. J. 422 (2009) 237-246]. The data on cytochrome bd are consistent with the observation that the purified enzyme has the heme d mainly in stable oxy-ferrous and ferryl states. The results are here discussed in the light of previously proposed models of the catalytic cycle of cytochrome bd. 相似文献