Effects of including endurance and speed sessions within small-sided soccer games periodization on physical fitness

The main aim of this study was to analyse the effects of including additional speed and endurance sessions during small-sided games (SSG) training periodization on physical fitness in professional soccer players. Sixteen outfield players (age = 25.6 ± 7.6 years) who competed in the First Division of a European League participated in this study. Players were randomly assigned to perform only the SSG periodization (G-SSG group) or to add endurance and speed training contents to the SSG (ES-SSG group). Before and after the 6-week experimental period, a Yo-Yo Intermittent Recovery Test level 1 (YYIR1) and a 40 m sprinting test were performed. Rating of perceived exertion (RPE) was also measured after each training session. The G-SSG group showed a large improvement in the YYIR1 performance (p = 0.018–0.028; ES = 0.521–0.576) after the training programme, whereas no significant changes were observed for the ES-SSG group (p = 0.763–1.000; ES = 0.000–0.014). In addition, no significant differences (p > 0.05, ES = 0.005–361, trivial to small) in sprint performance at 5 and 10 m intervals up to 40 m were observed at post-training in comparison to pre-training evaluation in G-SSG and ES-SSG groups. No significant differences (p > 0.05) between groups were observed at baseline in the YYIR1 test. The 6-week SSG training supported with only six endurance and speed training sessions was no more effective than well-organized SSG alone for improving running endurance in professional soccer players.     

A Mutant of Metarhizium anisopliae var. acridum with Enhanced Submerged Conidiation

Summary An insertional mutant of Metarhizium anisopliae is described with enhanced submerged conidiation. In a 500 ml submerged culture, this mutant produces a mean of 4.05 × 10⁸ propagules ml⁻¹ from an inoculum of 1 × 10⁶ conidia, where the parental strain accumulates only 3.75 × 10⁴ propagules ml⁻¹.     

Combining small-sided soccer games and running-based methods: A systematic review

Small-sided games (SSGs) are often used in soccer to produce acute physiological and physical responses, while a tactical/technical stimulus is also employed. However, due to some limitations of SSGs, researchers have been testing this method combined with running-based training methods. This systematic review was conducted to assess the effects of combined SSG and running-based methods on soccer players’ acute responses and adaptations after training interventions. A systematic review of Web of Science, PubMed, Cochrane Library, Scopus, and SPORTDiscus databases was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The database search initially identified 782 titles. From those, five articles were deemed eligible for the systematic review. The five included studies presented data from training load, reporting inconsistent greater values in combined SSG and running-based methods when compared to SSG-only formats. Considering the adaptations, studies comparing combined SSG and running-based methods with SSG-only methods revealed inconsistent differences in terms of the effects on aerobic performance and sprinting. Combining SSG and running-based methods can increase the acute mechanical load and high-intense running stimuli in players when compared to interventions that use only SSGs. However, the adaptations promoted by both methods are similar, and the differences are unclear. The order of combination (SSG and running-based method) does not seem to impact players’ adaptations; however, the frequency of sessions did have a meaningful impact.     

Assessment of schistosomiasis, through school surveys, in the Forest Zone of Pernambuco, Brazil

This work had the objective of assessing the present epidemiological situation regarding schistosomiasis through performing Kato-Katz coproscopic tests on representative samples of schoolchildren from each of the 43 municipality of endemic area of the state of Pernambuco, Brazil. The methodology is recommended by the World Health Organization to conduct sampled surveys among children at elementary school levels, ideal target group for baseline surveys: (i) schools are accessible; (ii) the greatest prevalence of schistosomiasis is found within this group; (iii) the data gathered from this age group can be used for intervention within the community as a whole. The following infection indicators were utilized: positivity (percentage of individuals examined with eggs of Schistosoma mansoni in the feces) and severity (geometric mean number of eggs per gram of feces, epg). These indicators allowed the area in general and the municipalities in particular to be categorized into prevalence and severity classes for S. mansoni. The prevalence classes were: low (<10%), medium (> 10 and < 50%), and high (> 50%); the severity classes were: low (1-99 epg), moderate (100-399 epg), and severe (> 400 epg). For the geohelminthic diseases, the following indicators were used: positivity for each geohelminth (percentage of individuals examined with eggs of geohelminths), and cumulative positivity (percentage of individuals examined with eggs of at least one geohelminth). The municipalities were categorized by means of their cumulative positivity into the following geohelminth prevalence classes (WHO 2002): low (< 50%), medium (> 50 and < 70%), and high (> 70%). The study covered 271 schools in 179 different localities, thus giving a total of 11,234 examinations performed. The overall positivity for S. mansoni was 14.4% and the egg count for this parasite in the feces gave a geometric mean of 67.9 epg which suggests a low general state of infection. These results allow this mesoregion to be categorized as presenting medium prevalence and low severity of schistosomiasis. The overall positivity rates for the geohelminths, Ascaris lumbricoides, Ancylostomidae, and Trichuris trichiura were, respectively, 30.4, 10.1, and 27.8%; the cumulative positivity was 45.4%. These results allow this mesoregion to be categorized as presenting low prevalence of geohelminthic diseases. The data show some municipalities in Pernambuco with prevalence greater than 20%, while others presented parasite loads greater than 100 epg. These indicators attest to the significant morbidity due to schistosomiasis regarding to the severity of infections established in young populations.     

Effect of acetonitrile on Cynara cardunculus L. cardosin A stability

The kinetics of the structural changes affecting cardosin A, a plant aspartic proteinase (AP) from Cynara cardunculus L., in the presence of a mixture of acetonitrile (AN) in water (W) was studied. Incubation of cardosin A with 10% (v/v) AN resulted in a gradual increase in protein helicity, accompanied by changes in the tertiary structure, seen by changes in the intrinsic fluorescence of tryptophan. Differential scanning calorimetry (DSC) revealed that the temperature of denaturation of cardosin A decreased upon the addition of AN. With longer incubation times, the small chain of cardosin A denatured completely, consequent exposure of the single tryptophan residue accounting well for the observed spectral shift intrinsic fluorescence of the protein. Enzymatic activity assays demonstrated that the kinetically determined unfolding of the small chain of cardosin A does not result in loss of the activity of this enzyme.     

Flood pulse regulation of bacterioplankton community composition in an Amazonian floodplain lake

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Occupancy, colonization and extinction patterns of rabbit populations: implications for Iberian lynx conservation

In Mediterranean ecosystems, rabbits are a key prey species for many predators, such as the Iberian lynx, which is threatened with extinction and has gone extinct locally in several regions of its historical distribution range. One of these regions is Serra da Malcata Nature Reserve, Portugal, which is also currently proposed as a potential site for reintroduction. We intended to investigate annual variation, potential time trends and the effects of management practices on the rabbit population in Serra da Malcata as a model for future potential reintroduction areas. The rabbit population was monitored over 12?years (from 1997 to 2009) by counting latrines along linear transects. These data were used to estimate rabbit occupancy, colonization and extinction patterns using a likelihood-based method including habitat, population and topographic covariate effects. Our results suggest that initial occupancy, when management practices were absent, was driven by the presence of Erica spp. and Cistus ladanifer shrubs and by distance to summits. Site colonization was positively influenced by the presence of edges between shrubs and pastureland and by patterns of rabbit distribution in the previous sampling season. On the other hand, local extinction was negatively influenced by edges. We conclude that the increase in rabbit occupancy and local colonization patterns was clearly associated with management actions (particularly, the creation of pasturelands), although the recovery of the species was noticeably limited by previous patterns of spatial distribution.     

Recovery of meiofauna following a short-term disturbance on coral reefs

The present study experimentally determined the short-term effect of different trampling intensities on the phytal habitat and associated meiofauna, and assessed the recovery of these communities on a coral reef. The experiment was conducted within the protected area on the reef of Porto de Galinhas Beach, northeastern Brazil. Within three areas, 30 × 30 cm² plots were subjected to different experimental trampling intensities: 0 (control), 32 (low intensity) and 79 (high intensity) footsteps applied during 3 consecutive days. Samples were taken before trampling started and then 1 day and 1, 2 and 3 months after the end of trampling. Multivariate and univariate analyses were used to test for changes in the phytal substrate, the densities of the main meiofaunal major taxa, and the diversity, comparing the trampling intensities and the time periods. The immediate impact of trampling negatively affected the phytal substrate, causing large reductions in sediment content, total substrate weight, and algal turf height. Trampling also drastically decreased the density of the associated meiofauna and modified the community structure. The major meiofauna groups showed different patterns of recovery during the first and second months. However, 3 months after trampling ended, no impact was found for densities of total meiofauna and for most major taxa, and no differences were observed in the community structure among all trampling intensities. Finally, the results for trampling susceptibility and resilience allowed us to extend the discussion to management strategies for the use of reef areas for recreation.     

Sampling plans for pest mites on physic nut

The starting point for generating a pest control decision-making system is a conventional sampling plan. Because the mites Polyphagotarsonemus latus and Tetranychus bastosi are among the most important pests of the physic nut (Jatropha curcas), in the present study, we aimed to establish sampling plans for these mite species on physic nut. Mite densities were monitored in 12 physic nut crops. Based on the obtained results, sampling of P. latus and T. bastosi should be performed by assessing the number of mites per cm² in 160 samples using a handheld 20× magnifying glass. The optimal sampling region for T. bastosi is the abaxial surface of the 4th most apical leaf on the branch of the middle third of the canopy. On the abaxial surface, T. bastosi should then be observed on the side parts of the middle portion of the leaf, near its edge. As for P. latus, the optimal sampling region is the abaxial surface of the 4th most apical leaf on the branch of the apical third of the canopy on the abaxial surface. Polyphagotarsonemus latus should then be assessed on the side parts of the leaf’s petiole insertion. Each sampling procedure requires 4 h and costs US$ 7.31.     

Net production and consumption of fluorescent colored dissolved organic matter by natural bacterial assemblages growing on marine phytoplankton exudates

An understanding of the distribution of colored dissolved organic matter (CDOM) in the oceans and its role in the global carbon cycle requires a better knowledge of the colored materials produced and consumed by marine phytoplankton and bacteria. In this work, we examined the net uptake and release of CDOM by a natural bacterial community growing on DOM derived from four phytoplankton species cultured under axenic conditions. Fluorescent humic-like substances exuded by phytoplankton (excitation/emission [Ex/Em] wavelength, 310 nm/392 nm; Coble's peak M) were utilized by bacteria in different proportions depending on the phytoplankton species of origin. Furthermore, bacteria produced humic-like substances that fluoresce at an Ex/Em wavelength of 340 nm/440 nm (Coble's peak C). Differences were also observed in the Ex/Em wavelengths of the protein-like materials (Coble's peak T) produced by phytoplankton and bacteria. The induced fluorescent emission of CDOM produced by prokaryotes was an order of magnitude higher than that of CDOM produced by eukaryotes. We have also examined the final compositions of the bacterial communities growing on the exudates, which differed markedly depending on the phytoplankton species of origin. Alteromonas and Roseobacter were dominant during all the incubations on Chaetoceros sp. and Prorocentrum minimum exudates, respectively. Alteromonas was the dominant group growing on Skeletonema costatum exudates during the exponential growth phase, but it was replaced by Roseobacter afterwards. On Micromonas pusilla exudates, Roseobacter was replaced by Bacteroidetes after the exponential growth phase. Our work shows that fluorescence excitation-emission matrices of CDOM can be a helpful tool for the identification of microbial sources of DOM in the marine environment, but further studies are necessary to explore the association of particular bacterial groups with specific fluorophores.