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801.
A. Alwin Prem Anand S. Gowri Sankar V. Kokila Vani 《Journal of cellular and molecular medicine》2012,16(11):2592-2610
Transplantation is common in clinical practice where there is availability of the tissue and organ. In the case of neurodegenerative disease such as Parkinson's disease (PD), transplantation is not possible as a result of the non‐availability of tissue or organ and therefore, cell therapy is an innovation in clinical practice. However, the availability of neuronal cells for transplantation is very limited. Alternatively, immortalized neuronal progenitors could be used in treating PD. The neuronal progenitor cells can be differentiated into dopaminergic phenotype. Here in this article, the current understanding of the molecular mechanisms involved in the differentiation of dopaminergic phenotype from the neuronal progenitors immortalized with SV40 LT antigen is discussed. In addition, the methods of generating dopaminergic neurons from progenitor cells and the factors that govern their differentiation are elaborated. Recent advances in cell‐therapy based transplantation in PD patients and future prospects are discussed. 相似文献
802.
Methylglyoxal (MG), a reactive α-oxoaldehyde, reacts with proteins to form irreversible advanced glycation end products (AGEs) following Maillard-like reaction. MG-induced AGE (MAGE) formation may be significant, particularly in diabetic condition with increased level of MG. Although myoglobin (Mb) is known to react with sugars to form AGEs, its interaction with MG is not known. Here we have studied interaction of Mb with MG. After in vitro reaction between Mb and MG at 25 °C for 7 days, the unchanged Mb and modified Mb (MG-Mb) were separated by ion exchange chromatography. Compared to Mb, MG-Mb exhibited higher electrophoretic mobility in native polyacrylamide gel electrophoresis, increased absorbance around 280 nm and more α-helical content, indicating structural changes of the modified protein. As shown by MALDI-mass spectrometry, MG converted Lys-16 and Lys-133 to carboxyethyllysine in MG-Mb. MAGE thus formed in MG-Mb may be associated with its enhanced mobility in native gel due to neutralization of positive charges and the observed structural changes in comparison with Mb. 相似文献
803.
Expression and processing of human rhinovirus type 14 polypeptide precursors in Escherichia coli maxicells 总被引:1,自引:0,他引:1
Human rhinovirus serotype-14 (HRV-14) cDNA, encompassing 87.9% of the coding region, was subcloned in an Escherichia coli expression vector, generating plasmid pKCC101. HRV-14 polypeptides encoded by pKCC101 were synthesized in E. coli maxicells. Pulse-chase experiments with pKCC110, a smaller derivative of pKCC101 containing the protease 3C coding region, have clearly demonstrated the proteolysis of a 55-kDa precursor to several polypeptides, including a doublet with the expected size of protease 3C (20 kDa). The proteolysis of the 55-kDa precursor polypeptide was prevented by ZnCl2, a known inhibitor of picornavirus 3C proteases. Results with a derivative of pKCC110 (pKCC115) which is partially deleted for the protease 3C sequence, support the idea that the doublet proteins are specified by the protease 3C coding region. Taken together, our investigations indicate that the precursor form of protease 3C must be responsible for its own cleavage. 相似文献
804.
Eileen Kröber Sonja Wende Saranya Kanukollu Caroline Buchen-Tschiskale Ludovic Besaury Frank Keppler Stéphane Vuilleumier Steffen Kolb Françoise Bringel 《Environmental microbiology》2021,23(8):4450-4465
Chloromethane (CH3Cl) is the most abundant halogenated volatile organic compound in the atmosphere and contributes to stratospheric ozone depletion. CH3Cl has mainly natural sources such as emissions from vegetation. In particular, ferns have been recognized as strong emitters. Mitigation of CH3Cl to the atmosphere by methylotrophic bacteria, a global sink for this compound, is likely underestimated and remains poorly characterized. We identified and characterized CH3Cl-degrading bacteria associated with intact and living tree fern plants of the species Cyathea australis by stable isotope probing (SIP) with 13C-labelled CH3Cl combined with metagenomics. Metagenome-assembled genomes (MAGs) related to Methylobacterium and Friedmanniella were identified as being involved in the degradation of CH3Cl in the phyllosphere, i.e., the aerial parts of the tree fern, while a MAG related to Sorangium was linked to CH3Cl degradation in the fern rhizosphere. The only known metabolic pathway for CH3Cl degradation, via a methyltransferase system including the gene cmuA, was not detected in metagenomes or MAGs identified by SIP. Hence, a yet uncharacterized methylotrophic cmuA-independent pathway may drive CH3Cl degradation in the investigated tree ferns. 相似文献
805.
806.
Giri Sib Sankar Jun Jin Woo Yun Saekil Kim Hyoun Joong Kim Sang Guen Kang Jeong Woo Kim Sang Wha Han Se Jin Park Se Chang Sukumaran V. 《Probiotics and antimicrobial proteins》2019,11(1):65-73
Probiotics and Antimicrobial Proteins - The present study was conducted to isolate and characterise Pb-resistant lactic acid bacteria (LAB), and thus determine their potential for use as probiotics... 相似文献
807.
RongsenKumzuk Deep Sankar Chini Manojit Bhattacharya Avijit Kar Shampa Patra Bidhan Chandra Patra 《Zeitschrift fur angewandte Ichthyologie》2020,36(2):249-250
The LWRs (length–weight relationships) of four freshwater fish species from medium categorized Doyang reservoir at Wokha District, Nagaland were studied, namely as Garra naganensis Hora, 1921; Devario naganensis Chaudhuri, 1912; Chagunius nicholsi Myers, 1924 and Schistura manipurensis Chaudhuri, 1912. Fish sample were collected in every 15 days interval by cast-nets (1.0 × 1.0 cm mesh size covering the 4.5 m2 areas), gill-nets (0.4–5.0 cm mesh size), scoop-nets (mesh size with 0.3 × 0.3 cm to 0.6 × 0.6 cm) and also from the local fishermen in between July 2017 to January 2019. The regression value of slope (b) is fallen into 2.860–3.115 range with significant correlation values (r2 ≥ .960). 相似文献
808.
Craig J. Thalhauser Tejas Sankar Mark C. Preul Yang Kuang 《Bulletin of mathematical biology》2009,71(3):585-601
We investigate a new model of tumor growth in which cell motility is considered an explicitly separate process from growth.
Bulk tumor expansion is modeled by individual cell motility in a density-dependent diffusion process. This model is implemented
in the context of an in vivo system, the tumor cord. We investigate numerically microscale density distributions of different
cell classes and macroscale whole tumor growth rates as functions of the strength of transitions between classes. Our results
indicate that the total tumor growth follows a classical von Bertalanffy growth profile, as many in vivo tumors are observed
to do. This provides a quick validation for the model hypotheses. The microscale and macroscale properties are both sensitive
to fluctuations in the transition parameters, and grossly adopt one of two phenotypic profiles based on their parameter regime.
We analyze these profiles and use the observations to classify parameter regimes by their phenotypes. This classification
yields a novel hypothesis for the early evolutionary selection of the metastatic phenotype by selecting against less motile
cells which grow to higher densities and may therefore induce local collapse of the vascular network. 相似文献
809.
810.
Bidisha Mukherjee Madhurina Majumdar Amitava Gangopadhyay Sankar Chakraborty Debashish Chaterjee 《International journal of phytoremediation》2015,17(7):651-656
Phytoremediation is an emerging technology applied for treatment of wastewater. It is a suitable option notably in developing countries as it is simple, sustainable and cost effective. In the present lab-based batch study the free floating aquatic plant water lettuce (Pistia stratiotes) is used for treatment of parboiled rice mill wastewater having low pH, high chemical oxygen demand (COD), nitrogen, and phosphate. In raw rice mill wastewater (undiluted) growth of water lettuce is found to be inhibited. Later on, two different dilution approaches (raw and facultative pond effluent 1:1; raw and tap water 1:1) are applied in order to effectively use this technology. In all cases a control (without plant) is maintained to compare the performance with the Aquatic Plant based Treatment (APT) system. In the APT system results reveal that removal of soluble COD (SCOD), ammoniacal nitrogen (NH4-N), nitrate nitrogen (NO3-N), and soluble phosphorus (sol. P) are upto 65%, 98%, 70%, and 65% respectively. The study highlights the efficacy of water lettuce in removing organics and nutrients from parboiled rice mill wastewater. 相似文献