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101.
Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells 总被引:1,自引:0,他引:1
In this study, we examined the hypothesis that stretch-induced (nitric oxide) NO modulates the mechanical properties of skeletal muscles by increasing accumulation of protein levels of talin and vinculin and by inhibiting calpain-induced proteolysis, thereby stabilizing the focal contacts and the cytoskeleton. Differentiating C2C12 myotubes were subjected to a single 10% step stretch for 04 days. The apparent elastic modulus of the cells, Eapp, was subsequently determined by atomic force microscopy. Static stretch led to significant increases (P < 0.01) in Eapp beginning at 2 days. These increases were correlated with increases in NO activity and neuronal NO synthase (nNOS) protein expression. Expression of talin was upregulated throughout, whereas expression of vinculin was significantly increased only on days 3 and 4. Addition of the NO donor L-arginine onto stretched cells further enhanced Eapp, NOS activity, and nNOS expression, whereas the presence of the NO inhibitor N-nitro-L-arginine methyl ester (L-NAME) reversed the effects of mechanical stimulation and of L-arginine. Overall, viscous dissipation, as determined by the value of hysteresis, was not significantly altered. For assessment of the role of vinculin and talin stability, cells treated with L-NAME showed a significant decrease in Eapp, whereas addition of a calpain inhibitor abolished the effect. Thus our results show that NO inhibition of calpain-initiated cleavage of cytoskeleton proteins was correlated with the changes in Eapp. Together, our data suggest that NO modulates the mechanical behavior of skeletal muscle cells through the combined action of increased talin and vinculin levels and a decrease in calpain-mediated talin proteolysis. mechanical stimulation; apparent elastic modulus; skeletal muscle cells; nitric oxide; stretch 相似文献
102.
The Common Nodulation Genes of Astragalus sinicus Rhizobia Are Conserved despite Chromosomal Diversity 总被引:1,自引:0,他引:1 下载免费PDF全文
Xue-Xian Zhang Sarah L. Turner Xian-Wu Guo Hui-Jun Yang Frdric Debell Guo-Ping Yang Jean Dnari J. Peter W. Young Fu-Di Li 《Applied microbiology》2000,66(7):2988-2995
The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria. 相似文献
103.
Sarah A. Cocherell Stephanie N. Chun Dennis E. Cocherell Lisa C. Thompson A. Peter Klimley Joseph J. CechJr. 《Environmental Biology of Fishes》2012,93(1):143-150
In regulated rivers, fluctuating water depths associated with pulsed discharges may strand small fish in side channels and
pools. Quantitative assessments of stranded fish are difficult in field studies (e.g., due to unknown effects of avian and
terrestrial vertebrate predators). To assess such lateral displacement and stranding on juvenile stream fishes, we designed,
constructed, and tested (with three species) a 2 × 1-m, lateral-displacement flume. The flume featured a main channel that
never drained and a raised, wide “floodplain” channel that alternately flooded, with a simulated pulse, and became dewatered.
The floodplain contained four pools, with different shapes and draining capacities, in which fish could become stranded as
the water level subsided. Fish-stranding rates (8%) in this relatively compact laboratory flume, after exposure to simulated
pulsed stream flows, were comparable to those observed in past investigations using larger, artificial streams. 相似文献
104.
Herpesviruses are double-stranded DNA, enveloped viruses that infect host cells through fusion with either the host cell plasma membrane or endocytic vesicle membranes. Efficient infection of host cells by herpesviruses is remarkably more complex than infection by other viruses, as it requires the concerted effort of multiple glycoproteins and involves multiple host receptors. The structures of the major viral glycoproteins and a number of host receptors involved in the entry of the prototypical herpesviruses, the herpes simplex viruses (HSVs) and Epstein-Barr virus (EBV), are now known. These structural studies have accelerated our understanding of HSV and EBV binding and fusion by revealing the conformational changes that occur on virus-receptor binding, depicting potential sites of functional protein and lipid interactions, and identifying the probable viral fusogen. 相似文献
105.
Christopher M. Aasted Meryem A. Yücel Sarah C. Steele Ke Peng David A. Boas Lino Becerra David Borsook 《PloS one》2016,11(11)
The purpose of this study was to use functional near-infrared spectroscopy (fNIRS) to examine patterns of both activation and deactivation that occur in the frontal lobe in response to noxious stimuli. The frontal lobe was selected because it has been shown to be activated by noxious stimuli in functional magnetic resonance imaging studies. The brain region is located behind the forehead which is devoid of hair, providing a relative ease of placement for fNIRS probes on this area of the head. Based on functional magnetic resonance imaging studies showing blood-oxygenation-level dependent changes in the frontal lobes, we evaluated functional near-infrared spectroscopy measures in response to two levels of electrical pain in awake, healthy human subjects (n = 10; male = 10). Each subject underwent two recording sessions separated by a 30-minute resting period. Data collected from 7 subjects were analyzed, containing a total of 38/36 low/high intensity pain stimuli for the first recording session and 27/31 pain stimuli for the second session. Our results show that there is a robust and significant deactivation in sections of the frontal cortices. Further development and definition of the specificity and sensitivity of the approach may provide an objective measure of nociceptive activity in the brain that can be easily applied in the surgical setting. 相似文献
106.
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108.
Melissa M. Page Kurtis D. Salway Yuen Kwong Ip Shit F. Chew Sarah A. Warren James S. Ballantyne Jeffrey A. Stuart 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2010,180(3):361-369
The African slender lungfish, Protopterus dolloi, is highly adapted to withstand periods of drought by secreting a mucous cocoon and estivating for periods of months to years. Estivation is similar to the diapause and hibernation of other animal species in that it is characterized by negligible activity and a profoundly depressed metabolic rate. As is typically observed in quiescent states, estivating P. dolloi are resistant to environmental stresses. We tested the hypothesis that P. dolloi enhances stress resistance during estivation by upregulating intracellular antioxidant defences in brain and heart tissues. We found that most of the major intracellular antioxidant enzymes, including the mitochondrial superoxide dismutase, cytosolic superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were upregulated in brain tissue of lungfish that had estivated for 60 days. Several of these enzymes were also elevated in heart tissue of estivators. These changes were not due to food deprivation, as they did not occur in a group of fish that were deprived of food but maintained in water for the same period of time. We found little evidence of tissue oxidative damage in estivators. Products of lipid peroxidation (4-hydroxynonenal adducts) and oxidative protein damage (carbonylation) were similar in estivating and control lungfish. However, protein nitrotyrosine levels were elevated in brain tissue of estivators. Taken together, these data indicate that estivating P. dolloi have enhanced oxidative stress resistance in brain and heart due to a significant upregulation of intracellular antioxidant capacity. 相似文献
109.
Selective precipitation and purification of monovalent proteins using oligovalent ligands and ammonium sulfate 总被引:1,自引:0,他引:1
Mirica KA Lockett MR Snyder PW Shapiro ND Mack ET Nam S Whitesides GM 《Bioconjugate chemistry》2012,23(2):293-299
This paper describes a method for the selective precipitation and purification of a monovalent protein (carbonic anhydrase is used as a demonstration) from cellular lysate using ammonium sulfate and oligovalent ligands. The oligovalent ligands induce the formation of protein-ligand aggregates, and at an appropriate concentration of dissolved ammonium sulfate, these complexes precipitate. The purification involves three steps: (i) the removal of high-molecular-weight impurities through the addition of ammonium sulfate to the crude cell lysate; (ii) the introduction of an oligovalent ligand and the selective precipitation of the target protein-ligand aggregates from solution; and (iii) the removal of the oligovalent ligand from the precipitate by dialysis to release the target protein. The increase of mass and volume of the proteins upon aggregate formation reduces their solubility, and results in the selective precipitation of these aggregates. We recovered human carbonic anhydrase, from crude cellular lysate, in 82% yield and 95% purity with a trivalent benzene sulfonamide ligand. This method provides a chromatography-free strategy of purifying monovalent proteins--for which appropriate oligovalent ligands can be synthesized--and combines the selectivity of affinity-based purification with the convenience of salt-induced precipitation. 相似文献
110.
Hominin-cercopithecid comparisons have been used in palaeoanthropology for over forty years. Fossil cercopithecids can be used as a 'control group' to contextualize the adaptations and evolutionary trends of hominins. Observations made on modern cercopithecids can also be applied to questions about human evolution. This article reviews the history of hominin-cercopithecid comparisons, assesses the strengths and weaknesses of cercopithecids as comparators in studies of human evolution, and uses cercopithecid models to explore hominin inter-specific dynamics. Cercopithecids appear to be excellent ecological referents, but may be less good when considering the cognitive abilities and cultural adaptations of hominins. Comparison of cercopithecid and hominin adaptations at Koobi Fora in East Africa indicates that, whereas the cercopithecids were largely grass- or leaf-eating, the hominins occupied a generalist niche, apparently excluding other primate generalist-frugivores. If any of the hominin species at Koobi Fora were sympatric, analogies with modern cercopithecids suggest that inter-specific contact cannot be discounted and may even have been beneficial. 相似文献