The transport of oxidized glutathione from the erythrocytes of various species in the prescence of chromate

1. Erythrocytes from normal and glucose 6-phosphate dehydrogenase-deficient humans were subjected to hydrogen peroxide diffusion to oxidize the GSH. Studies were carried out in the presence and absence of chromate to inhibit glutathione reductase and with or without the addition of glucose. 2. The GSH content of erythrocytes from other species was oxidized by subjecting them to hydrogen peroxide diffusion in the presence of chromate and glucose. 3. Chromate (1.3mm) inhibited glutathione reductase by about 80%, whereas glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphofructokinase and pyruvate kinase were not inhibited. 4. The GSSG formed was transported from the erythrocytes to the medium. 5. The transport rate of GSSG from glucose 6-phosphate dehydrogenase-deficient erythrocytes subjected to hydrogen peroxide diffusion in the presence of chromate was comparable with that from normal and glucose 6-phosphate dehydrogenase-deficient erythrocytes. 6. The rate of transport of GSSG from erythrocytes of various species studied could be ranked: pigeon>rabbit>rat>donkey>man>dog>horse>sheep>chicken>fish.     

Ultra-violet induced mutations to growth factor requirement and penicillin resistance in a blue-green alga

Summary After exposures to UV, two mutant strains of Phormidium mucicola were isolated which are stable and inheritable:1. Strain 5/5 mL-1 is a photoheterotrophic mutant requiring addition of an organic growth factor to the basal medium which appears fulfilled by either casein hydrolysate, glucose, acetate or vitamin mixture and fails to grow heterotrophically.2. mr/p. This strain shows resistance upto 10.24 g/ml of penicillin whereas the parent does not survive beyond a penicillin dose of 0.1 g/ml.