全文获取类型
收费全文 | 5003篇 |
免费 | 334篇 |
国内免费 | 5篇 |
专业分类
5342篇 |
出版年
2023年 | 30篇 |
2022年 | 48篇 |
2021年 | 91篇 |
2020年 | 91篇 |
2019年 | 54篇 |
2018年 | 170篇 |
2017年 | 140篇 |
2016年 | 199篇 |
2015年 | 248篇 |
2014年 | 235篇 |
2013年 | 354篇 |
2012年 | 359篇 |
2011年 | 360篇 |
2010年 | 228篇 |
2009年 | 181篇 |
2008年 | 285篇 |
2007年 | 273篇 |
2006年 | 235篇 |
2005年 | 221篇 |
2004年 | 209篇 |
2003年 | 224篇 |
2002年 | 167篇 |
2001年 | 83篇 |
2000年 | 81篇 |
1999年 | 65篇 |
1998年 | 44篇 |
1997年 | 32篇 |
1996年 | 27篇 |
1995年 | 31篇 |
1994年 | 32篇 |
1993年 | 17篇 |
1992年 | 36篇 |
1991年 | 29篇 |
1990年 | 37篇 |
1989年 | 37篇 |
1988年 | 23篇 |
1987年 | 18篇 |
1986年 | 21篇 |
1985年 | 22篇 |
1984年 | 41篇 |
1983年 | 20篇 |
1982年 | 20篇 |
1981年 | 31篇 |
1980年 | 20篇 |
1979年 | 16篇 |
1978年 | 12篇 |
1977年 | 12篇 |
1975年 | 12篇 |
1972年 | 11篇 |
1971年 | 11篇 |
排序方式: 共有5342条查询结果,搜索用时 15 毫秒
71.
Five novel representatives of the yeast genus Candida isolated from advanced stages of wood degradation from fallen trunks of Eucryphia cordifolia Cav., Laurelia sempervirens Weim., Laurelia philippiana Weim., Nothofagus dombeyii (Mirb.) Blume, and Nothofagus obliqua (Mirb.) Blume, in the evergreen rainy Valdivian forest of southern Chile, are described and illustrated. They clearly differ from all accepted Candida species to warrant their establishment as five new species of yeasts: Candida coipomensis sp. nov., Candida ralunensis sp. nov., Candida laureliae sp. nov., Candida osornensis sp. nov., and Candida llanquihuensis sp. nov. 相似文献
72.
Four unusual Apiotrichum, isolated from decayed wood of Eucryphia cordifolia Cav., Nothofagus obliqua (Mirb.) Blume, and Laurelia sempervirens Wein., one of which was also isolated from the intestinal tract of Scaptomyza multispinosa Malloch (Diptera), are described and illustrated. These species differ from all the accepted Apiotrichum species (1–3) to warrant their establishment as four new species: Apiotrichum eucryphiae, Apiotrichum osvaldii, Apiotrichum futronensis and Apiotrichum nothofagi. 相似文献
73.
Two unusual species of Candida and one variety of one of them, isolated from decayed wood (palo podrido) (12) in the ultimate stages of degradation, are described and illustrated. They differ sufficiently from all accepted Candida species to warrant their establishment as two new species and one variety: Candida railenensis, Candida bertae, and Candida bertae var. chiloensis.The DNA base composition of these yeasts was not calculated, since our laboratory is not properly equipped for that purpose. 相似文献
74.
Two new compounds G and H isolated from the roots of Costus speciosus have been characterized as 8-hydroxytriacontan-25-one and Me tritriacontanoat 相似文献
75.
Characterization and Translation of Poly(A)+RNA from Wounded and Crown Gall Tissues of Potato Tubers
Poly(A)+ and poly(A)RNA from wounded potato tuber tissuesand crown gall tumors were separated from total RNA by oligodeoxythymidylicacid-cellulose affinity chromatography. The poly(A)+RNA wascharacterized by sucrose density gradient centrifugation, hybridizationwith 3(H)polyuridylic acid [Poly(U)] and in vitro translationin a rabbit reticulocyte lysate system. The tumor poly(A)+RNAwas a heterodisperse mixture from 3.5S to 35S. Upon poly(U)hybridization of the gradient fractions two major hybridizationpeaks at 7S and 21S and two peaks at 11S and 16S appeared. Inan in vitro translation system the poly(A)+RNA programmed thesynthesis of 23 different polypeptides of 9,000 to 79,800 daltonsmolecular weight as determined by SDS-polyacrylamide gel electrophoresis.The 21S poly(A)+RNA was about 5 times more active in in vitroprotein synthesis than the 7S poly(A)+RNA. The poly(A)+RNA from wounded tissues was also heterodisperse(from 4.5S to 31S) with a modal peak at 18S. This RNA codedfor at least 28 polypeptides, which were different from thoseof crown gall tumor tissues. On a per unit poly(A)+RNA basis the tumor RNA was slightly moreactive in translation than that from wounded tissues. The translationof tumor poly(A)+RNA was completely blocked by 0.5 mM 7-methylguanosine5'-phosphate, but not by 7-methylguanosine, suggesting the presenceof a 5'-cap structure. (Received May 15, 1982; Accepted June 30, 1982) 相似文献
76.
Six new species ofPenicillium Linkex Fries are described and illustrated. Four of them have been recovered from the atmosphere in Madrid, Spain, the other two species were isolated from must and from soil respectively. They clearly differ from all species of the genus described so far and are, therefore described and proposed as new taxa:Penicillium gerundense sp. nov.,Penicillium valentinum sp. nov.,Penicillium alicantinum sp. nov.,Penicillium malacaense sp. nov.,Penicillium tarraconense sp. nov., andPenicillium vasconiae sp. nov. 相似文献
77.
Four new species of Penicillium Link ex Fries are described and illustrated. All of them but one have been isolated from the atmosphere in Madrid, Spain. They clearly differ from all species of the genus described so far and are, therefore, described and proposed as new taxa: Penicillium aurantio-flammiferum sp. nov., Penicillium gallaicum sp. nov., Penicillium granatense sp. nov., and Penicillium ilerdanum sp. nov. 相似文献
78.
During a two-year survey on the air-borne fungi in the atmosphere of Barcelona (Spain), the following genera were isolated in decreasing order: Aureobasidium, Rhizopus, Mucor, Arthrinium, Phoma, Fusarium, Trichoderma, and Botrytis. 相似文献
79.
Two strains ofAspergillus flavus Linkex Fr. and two strains ofA. parasiticus Speare were cultured on crushed moist wheat (Triticum durum var. Pané no. 247) for aflatoxin production studies in correlation with morphological changes. The toxicogenic strains were adapted to the substratum by means of successive transfers at regular intervals (72 h.)The amount aflatoxins synthesized by the toxicogenic strains decreased gradually after succesive subculturing. The decrease was accompanied by marked morphological changes. One of the strains studied,A. flavus NRRL 3251, lost completly the capacity of aflatoxin synthesis after several subcultures, presenting at the same time strong morphological variations.A. flavus CBS 120.62 also lost its toxicogenicity after six subcultures. 相似文献
80.
Abstract— The effects of feeding dietary wheat and Bengal gram proteins to pregnant rats on brain protein and glutamic acid metabolism in 15-, 17- and 19-day fetuses were investigated. Wheat and Bengal gram diets resulted in loss of brain weight with decreased DNA, RNA, protein, free x amino N and deficits in the activities of brain glutamine synthetase, glutaminase I. glutaminase II and glutamate decarboxylase at all the gestational ages studied without any change in glutamine transferase activity. The concentrations of the amino acids alanine, glutamic acid, glutamine and GABA were found to be significantly lower on wheat and Bengal gram diets than the control on a 10% casein diet. The wheat with lysine and Bengal gram with methionine, cystine and tryptophan resulted in similar mean values of all the characteristics studied to the mean values observed in rats on the control diet. However, glutaminase I activity remained significantly low on lysine fortified wheat diet, and aspartic acid content was found to increase on both fortified and unfortified wheat and Bengal gram diets. A 20% casein diet showed increased brain weight, DNA. RNA. protein and free x amino N concentrations as compared with the 10% casein diet, while the other parameters remained unchanged. 相似文献