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991.
992.
Background
Neuroblastoma is the most common extracranial pediatric solid tumor. Intermittent hypoxia, which is characterized by cyclic periods of hypoxia and reoxygenation, has been shown to positively modulate tumor development and thereby induce tumor growth, angiogenic processes, and metastasis. Bone is one of the target organs of metastasis in advanced neuroblastoma Neuroblastoma cells produce osteoclast-activating factors that increase bone resorption by the osteoclasts. The present study focuses on how intermittent hypoxia preconditioned SH-SY5Y neuroblastoma cells modulate osteoclastogenesis in RAW 264.7 cells compared with neuroblastoma cells grown at normoxic conditions.Methods
We inhibited HIF-1α and HIF-2α in neuroblastoma SH-SY5Y cells by siRNA/shRNA approaches. Protein expression of HIF-1α, HIF-2α and MAPKs were investigated by western blotting. Expression of osteoclastogenic factors were determined by real-time RT-PCR. The influence of intermittent hypoxia and HIF-1α siRNA on migration of neuroblastoma cells and in vitro differentiation of RAW 264.7 cells were assessed. Intratibial injection was performed with SH-SY5Y stable luciferase-expressing cells and in vivo bioluminescence imaging was used in the analysis of tumor growth in bone.Results
Upregulation of mRNAs of osteoclastogenic factors VEGF and RANKL was observed in intermittent hypoxia-exposed neuroblastoma cells. Conditioned medium from the intermittent hypoxia-exposed neuroblastoma cells was found to enhance osteoclastogenesis, up-regulate the mRNAs of osteoclast marker genes including TRAP, CaSR and cathepsin K and induce the activation of ERK, JNK, and p38 in RAW 264.7 cells. Intermittent hypoxia-exposed neuroblastoma cells showed an increased migratory pattern compared with the parental cells. A significant increase of tumor volume was found in animals that received the intermittent hypoxia-exposed cells intratibially compared with parental cells.Conclusions
Intermittent hypoxic exposure enhanced capabilities of neuroblastoma cells in induction of osteoclast differentiation in RAW 264.7 cells. Increased migration and intratibial tumor growth was observed in intermittent hypoxia-exposed neuroblastoma cells compared with parental cells. 相似文献993.
To address the question whether the abundance of an invasive species can be explained by physical and chemical properties
of the invaded ecosystems, we gathered density data of invasive zebra mussels and the physical and chemical data of ecosystems
they invaded. We assembled published data from 55 European and 13 North American lakes and developed a model for zebra mussel
density using a generalized additive model (GAM) approach. Our model revealed that the joint effect of surface area, total
phosphorus and calcium concentrations explained 62% of the variation in Dreissena density. Our study indicates that large and less productive North American lakes can support larger local populations of
zebra mussels. Our results suggest that the proliferation of an exotic species in an area can partially be explained by physical
and chemical properties of the recipient environment. 相似文献
994.
Anantha R. Nookala Junhao Li Anusha Ande Lei Wang Naveen K. Vaidya Weihua Li Santosh Kumar Anil Kumar 《PloS one》2016,11(1)
Cytochrome P450 3A4 (CYP3A4) is the major drug metabolic enzyme, and is involved in the metabolism of antiretroviral drugs, especially protease inhibitors (PIs). This study was undertaken to examine the effect of methamphetamine on the binding and metabolism of PIs with CYP3A4. We showed that methamphetamine exhibits a type I spectral change upon binding to CYP3A4 with δAmax and KD of 0.016±0.001 and 204±18 μM, respectively. Methamphetamine-CYP3A4 docking showed that methamphetamine binds to the heme of CYP3A4 in two modes, both leading to N-demethylation. We then studied the effect of methamphetamine binding on PIs with CYP3A4. Our results showed that methamphetamine alters spectral binding of nelfinavir but not the other type I PIs (lopinavir, atazanavir, tipranavir). The change in spectral binding for nelfinavir was observed at both δAmax (0.004±0.0003 vs. 0.0068±0.0001) and KD (1.42±0.36 vs.2.93±0.08 μM) levels. We further tested effect of methamphetamine on binding of 2 type II PIs; ritonavir and indinavir. Our results showed that methamphetamine alters the ritonavir binding to CYP3A4 by decreasing both the δAmax (0.0038±0.0003 vs. 0.0055±0.0003) and KD (0.043±0.0001 vs. 0.065±0.001 nM), while indinavir showed only reduced KD in presence of methamphetamine (0.086±0.01 vs. 0.174±0.03 nM). Furthermore, LC-MS/MS studies in high CYP3A4 human liver microsomes showed a decrease in the formation of hydroxy ritonavir in the presence of methamphetamine. Finally, CYP3A4 docking with lopinavir and ritonavir in the absence and presence of methamphetamine showed that methamphetamine alters the docking of ritonavir, which is consistent with the results obtained from spectral binding and metabolism studies. Overall, our results demonstrated differential effects of methamphetamine on the binding and metabolism of PIs with CYP3A4. These findings have clinical implication in terms of drug dose adjustment of antiretroviral medication, especially with ritonavir-boosted antiretroviral therapy, in HIV-1-infected individuals who abuse methamphetamine. 相似文献
995.
Khaled M. Abdel Rahman Marek Barta Ľudovít Cagáň 《Central European Journal of Biology》2010,5(4):472-480
We tested the combined effect of the fungus Beauveria bassiana and the microsporidium Nosema pyrausta on the European corn borer larvae, Ostrinia nubilalis, in the laboratory. The first instar of O. nubilalis larvae was the most sensitive to the B. bassiana infection followed by the fifth, second, third, and fourth instar (LC50s were 4.91, 6.67, 7.13, 9.15, and 6.51 × 105 conidia/ml for the first to fifth instars, respectively). Mortality of each instar increases positively with concentration
of conidia. When B. bassiana and N. pyrausta were used in combination, mortality increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16%, 37.63%, 21.60%, 27.11%, and 33.95% for the first to fifth instars, respectively. The combined effects of the
two pathogens were mostly additive. However, at the two highest concentrations the pathogens interacted synergistically in
the first and second instar. Individuals that survived the B. bassiana and B. bassiana + N. pyrausta treatments and developed into adults had significantly shorter lifespans and females oviposited fewer eggs than non-exposed
insects. The effects on the longevity and the egg production were most pronounced at high concentration of B. bassiana conidia. 相似文献
996.
James S. Wakeley Michael P. Guilfoyle Terry J. Antrobus Richard A. Fischer Wylie C. BarrowJr. Paul B. Hamel 《Wetlands Ecology and Management》2007,15(5):417-439
We used an ordination approach to identify factors important to the organization of breeding bird communities in three floodplains:
Cache River, Arkansas (AR), Iatt Creek, Louisiana (LA), and the Coosawhatchie River, South Carolina (SC), USA. We used 5-min
point counts to sample birds in each study area each spring from 1995 to 1998, and measured ground-surface elevations and
a suite of other habitat variables to investigate bird distributions and community characteristics in relation to important
environmental gradients. In both AR and SC, the average number of Neotropical migrant species detected was lowest in semipermanently
flooded Nyssa aquatica Linnaeus habitats and greatest in the highest elevation floodplain zone. Melanerpes carolinus Linnaeus, Protonotaria citrea Boddaert, Quiscalus quiscula Linnaeus, and other species were more abundant in N. aquatica habitats, whereas Wilsonia citrina Boddaert, Oporornis formosus Wilson, Vireo griseus Boddaert, and others were more abundant in drier floodplain zones. In LA, there were no significant differences in community
metrics or bird species abundances among forest types. Canonical correspondence analyses revealed that structural development
of understory vegetation was the most important factor affecting bird distributions in all three study areas; however, potential
causes of these structural gradients differed. In AR and SC, differences in habitat structure were related to the hydrologic
gradient, as indexed by ground-surface elevation. In LA, structural variations were related mainly to the frequency of canopy
gaps. Thus, bird communities in all three areas appeared to be organized primarily in response to repeated localized disturbance.
Our results suggest that regular disturbance due to flooding plays an important role in structuring breeding bird communities
in floodplains subject to prolonged inundation, whereas other agents of disturbance (e.g., canopy gaps) may be more important
in headwater systems subject to only short-duration flooding. Management for avian community integrity in these systems should
strive to maintain forest zonation and natural disturbance regimes. 相似文献
997.
Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content 总被引:4,自引:0,他引:4
Weselake RJ Shah S Tang M Quant PA Snyder CL Furukawa-Stoffer TL Zhu W Taylor DC Zou J Kumar A Hall L Laroche A Rakow G Raney P Moloney MM Harwood JL 《Journal of experimental botany》2008,59(13):3543-3549
Top-down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic pathways that might be overcome by biotechnological approaches. Previous studies on lipid accumulation in oilseed rape have suggested that diacylglycerol acyltransferase (DGAT), which catalyses the final step in seed oil biosynthesis, might be an effective target for enhancing seed oil content. Here, increased seed oil content, increased DGAT activity, and reduced substrate:product ratio are demonstrated, as well as reduced flux control by complex lipid assembly, as determined by TDCA in Brassica napus (canola) lines which overexpress the gene encoding type-1 DGAT. Lines overexpressing DGAT1 also exhibited considerably enhanced seed oil content under drought conditions. These results support the use of TDCA in guiding the rational selection of molecular targets for oilseed modification. The most effective lines had a seed oil increase of 14%. Moreover, overexpression of DGAT1 under drought conditions reduced this environmental penalty on seed oil content. 相似文献
998.
999.
Biological availability and humic properties of dissolved organic carbon in Lake Balaton (Hungary) 总被引:1,自引:0,他引:1
The nursery role of the Mondego estuary for marine fish species was studied between June 2003 and May 2004. The spatial and
temporal distribution and abundance patterns of 0-group Dicentrarchus
labrax (Linnaeus, 1758), Platichthys
flesus (Linnaeus, 1758) and Solea solea (Linnaeus, 1758) were analyzed based on monthly sampling surveys in five stations along the estuarine gradient. Fishing took
place during the night at low water of spring tides, using a 2 m beam trawl. The spatial patterns of estuarine colonization
were different according to species. D. labrax showed a wider distribution, but the main nursery ground was the same as for S. solea. Highest densities of S. solea juveniles were found in oligohaline areas, with muddy bottoms and high benthic invertebrates availability, while P. flesus occurred mainly in the sandy uppermost areas. D. labrax was found in both these areas. Fish abundance in the estuary mainly reflected seasonal changes. 相似文献
1000.
Mikael Gyllström Thomas Lakowitz Christer Brönmark Lars-Anders Hansson 《Ecosystems》2008,11(7):1120-1132
In an experimental study we assessed if benthic bioturbating invertebrates affect the recruitment (hatching) of zooplankton
from the sediment, and if this effect persists as differences in the zooplankton community in the water column, that is, if
bioturbation quantitatively stimulates benthic–pelagic coupling. We investigated the effects of four different benthic invertebrates
(Asellus aquaticus, Chironomus plumosus, Tubifex tubifex in the presence or absence of the predator Sialis lutaria). In total, 45 zooplankton taxa hatched from the sediment and the hatching success of some of these was dependent on the
species identity of the bioturbating invertebrate. The predator Sialis reduced the abundance of all three invertebrate species, but tended to positively influence the zooplankton recruitment rates,
possibly through increasing the activity of the bioturbating invertebrates. The most striking effect of bioturbation on the
hatching and pelagic zooplankton community properties was that, on average, 11% more species hatched in the Asellus treatment than in any other treatment. This was also mirrored in the zooplankton water column community where, on average,
7% more species established a viable population in treatments with Asellus as bioturbator. In a complementary field survey, Asellus was more common in littoral than in profundal sediments. Because Asellus strongly affected recruitment of zooplankton in our experiment, we argue that bioturbation may partly explain why recruitment
of resting stages of both phyto- and zooplankton is generally higher in littoral than in profundal areas. 相似文献