排序方式: 共有459条查询结果,搜索用时 15 毫秒
451.
Jelena Veki Jasmina Ivanievi Aleksandra Zeljkovi Vesna Spasojevi-Kalimanovska Nataa Bogavac-Stanojevi Marija Mihajlovi Jelena Jana Sanja Vuj
i Milica Miljkovi Dejan Zujovi Jelena Kotur-Stevuljevi 《Journal of Medical Biochemistry》2020,39(4):467
BackgroundPropolis and N-acetylcysteine have positive impact on respiratory tract health. Also, it has been suggested that they have beneficial effects on serum lipid and oxidative stress status, but the available data are limited and mostly gained from animal models. In this study we evaluated the effects of propolis and N-acetylcysteine supplementation (PropoMucil®) on lipid status, lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection.MethodsTwenty subjects with acute respiratory infection were included. PropoMucil® granules for oral solution (80 mg of dry propolis extract and 200 mg of N-acetylcysteine) were administered tree times per day for ten days. Serum lipid profile, paraoxonase 1 activity and low-density and high-density lipoprotein size and subclasses distribution were assessed at baseline and after supplementation.ResultsFollowing ten days of supplementation lipid status remained unchanged, but a significant increase of low-density lipoprotein particle size and proportion of high-density lipoprotein 3a particles were found (P<0.05). Moreover, supplementation with PropoMucil® significantly improved high-density lipoprotein particles distribution, particularly in those who smoke. There was a moderate increase of paraoxonase 1 activity, but without statistical significance.ConclusionsThe presented study demonstrated that short-term supplementation with PropoMucil® has beneficial effects on low-density and high-density lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection particularly in those who smoke. 相似文献
452.
Sanja Milovi Ivan Stankovi Dejan Nikoli Jelena Radovi Marina Kolundi Vesna Nikoli Tatjana Stanojkovi Slavica Petovi Tatjana Kundakovi‐Vasovi 《化学与生物多样性》2019,16(10)
Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7‐sulfate, caftaric and coutaric acid). The content of β‐glucan, fatty acids, sterols and micro‐ and macro‐elements were investigated among all samples. The highest content of β‐glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β‐sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively). 相似文献
453.
Combined metabolic activity within an atrazine-mineralizing community enriched from agrochemical factory soil 总被引:1,自引:0,他引:1
Nikolina Udikovi Koli Dubravka Hrak Ana Begonja Kolar Ines Petri Sanja Stipi
evic Guy Soulas Fabrice Martin-Laurent 《International biodeterioration & biodegradation》2007,60(4):299-307
The main objective of this work was to characterize an atrazine-mineralizing community originating from agrochemical factory soil, especially to elucidate the catabolic pathway and individual metabolic and genetic potentials of culturable members. A stable four-member bacterial community, characterized by colony morphology and 16S rDNA sequencing, was rapidly able to mineralize atrazine to CO2 and NH3. Two primary organisms were identified as Arthrobacter species (ATZ1 and ATZ2) and two secondary organisms (CA1 and CA2) belonged to the genera Ochrobactrum and Pseudomonas, respectively. PCR assessment of atrazine-degrading genetic potential of the community, revealed the presence of trzN, trzD, atzB and atzC genes. Isolates ATZ1 and ATZ2 were capable of dechlorinating atrazine to hydroxyatrazine and contained the trzN gene. ATZ2 further degraded hydroxyatrazine to cyanuric acid and contained atzB and atzC genes whereas ATZ1 contained atzC but not atzB. Isolates CA1 and CA2 grew on cyanuric acid and contained the trzD gene. Complete atrazine degradation was a result of the combined metabolic attack on the atrazine molecule, and complex interactions may exist between the community members sharing carbon and nitrogen from atrazine mineralization.Scientific relevance: Despite numerous reports on atrazine degradation by pure bacterial cultures, the pathways and the atrazine-degrading gene combinations harboured by bacterial communities are only poorly described. In this work, we characterized a four-member atrazine-mineralizing community enriched from an agrochemical factory soil, which was capable of rapidly metabolizing atrazine to CO2. This study will contribute towards better understanding of the genetic potential and metabolic activities of atrazine-degrading communities, which are generally considered to be responsible for atrazine mineralization in the natural environment. 相似文献
454.
Sanja Perovic Jürgen Seack Vera Gamulin Werner EG Müller Heinz C Schröder 《BMC cell biology》2001,2(1):7-9
Background
Ethylene is a widely distributed alkene product which is formed enzymatically (e.g., in plants) or by photochemical reactions (e.g., in the upper oceanic layers from dissolved organic carbon). This gaseous compound was recently found to induce in cells from the marine sponge Suberites domuncula, an increase in intracellular Ca2+ level ([Ca2+]i) and an upregulation of the expression of two genes, the potential ethylene-responsive gene, SDERR, and a Ca2+/calmodulin-dependent protein kinase. 相似文献455.
Jelena Godrijan Daniela Marić Pfannkuchen Tamara Djakovac Sanja Frka Blaženka Gašparović 《Global Change Biology》2023,29(17):5075-5086
The future of life on Earth depends on how the ocean might change, as it plays an important role in mitigating the effects of global warming. The main role is played by phytoplankton. Not only are phytoplankton the base of the oceans' food web, but they also play an important role in the biological carbon pump (BCP), the process of forming organic matter (OM) and transporting it to the deep sea, representing a sink of atmospheric CO2. Lipids are considered important vectors for carbon sequestration. A change in the phytoplankton community composition as a result of ocean warming is expected to affect the BCP. Many predictions indicate a dominance of small at the expense of large phytoplankton. To gain insight into interplay between the phytoplankton community structure, lipid production and degradation, and adverse environmental conditions, we analyzed phytoplankton composition, particulate organic carbon (POC) and its lipid fraction in the northern Adriatic over a period from winter to summer at seven stations with a gradient of trophic conditions. We found that at high salinity and low nutrient content, where nanophytoplankton prevailed over diatoms, the newly fixed carbon is substantially directed toward the synthesis of lipids. Lipids produced by nanophytoplankton, coccolithophores, and phytoflagellates, are more resistant to degradation than those produced by diatoms. The difference in lipid degradability is discussed as a difference in the size of the cell phycosphere. We hypothesize that the lipids of nanophytoplankton are less degradable due to the small phycosphere with a poorer bacterial community and consequently a lower lipid degradation rate compared with diatoms. The lipid chemical composition of the different phytoplankton groups could have a different susceptibility to degradation. Results suggest a successful lipid carbon sink of nanophytoplankton and, thus, a negative feedback on global warming. 相似文献
456.
Emina ?olak Dragana Pap Ljubinka Nikoli? Sanja Vickovi? 《Journal of Medical Biochemistry》2020,39(3):346
BackgroundThe goal of this study was to assess the oxidative stress status through the values of antioxidant defense parameters: superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and total antioxidant status (TAS), as well as cardiovascular risk factors (total cholesterol, LDL-cholesterol, VLDL-cholesterol, non-HDL-cholesterol and triglycerides), anthropometric parameters (Body mass index-BMI, waist circumference-WC, hipp circumferemce-HC, waist-to-hipp ratio-WHR and inflammatory markers (high sensitive C-reactive protein) in a group of obese adolescents.MethodsA total of 238 students of both sexes, age of 22.32 ± 1.85 yr. were included in the study. According to the values of BMI lower and higher than 25 kg/m2 and WC higher and lower than 94 cm (males)/80 cm (females) the tested group of students was divided into 2 subgroups: Group 1 (increased risk for CVD) and Group 2 (lower risk for CVD).ResultsSignificantly reduced SOD and GPx with increased GR, TAS, inflammatory and lipoprotein parameters were obtained in Group 1 compared to Group 2. Significant positive association of hsCRP (OR:1.41; 95%CI 1.08-1.83; P=0.007), TAS (OR:827.2; 95%CI 19.27-35498; P=0.007) and GR (OR:1.13; 95%CI 1.05-1.21; P=0.002) and negative association of GPx (OR:0.97; 95%CI 0.94-1.003; P=0.043) and HDL-cholesterol (OR:0.41; 95%CI 0.176-0.963; P=0.0014) with cardiovascular risk factors were found in obese students. According to the ROC analysis GR>44.8 U/L, TAS>1.35 mmol/L, hsCRP>1.71 mg/L and HDL-cholesterol <1.13 mmol/L have sufficient predictive ability for cardiovascular disease in obese students.ConclusionsSignificant association of antioxidant defense parameters with anthropometric, lipid and inflammatory markers in obese students with increased cardiovascular risk suggest that screening of these parameters is necessary and highly recommended. 相似文献
457.
Daria Hafner Sanja Vujisic Zivkovic Renato Bauman Kujundzic Tiljak Neven Papić Snjezana Zidovec Lepej 《Reproductive biology》2018,18(3):289-294
The aim of this study was to analyse the presence of vascular endothelial growth factor (VEGF) and interferon alpha (IFN-α) in the follicular fluid (FF) and their possible influence, as pro-angiogenic or anti-angiogenic factors, on in vitro fertilization outcome. The concentrations of VEGF and IFN-α were correlated with oocyte and embryo quality, concentrations of hormones in the serum, perifollicular blood flow and endometrial thickness. VEGF was detected in all FF samples (median 706.6?pg/ml, range 182.9–6638?pg/ml). IFN-α was detected in 60% of the samples (median 6.5?pg/ml, range 0–79.4?pg/ml), while in 40% of the samples its levels were below the test detection limit. VEGF and IFN-α concentrations did not correlate with the cause of infertility, concentrations of FSH, LH, E2 and prolactin, oocyte or embryo quality. Significantly higher concentrations of VEGF have been found in women with primary compared with secondary infertility (p?=?0.011, Mann Whitney test). The concentrations of VEGF and IFN-α did not correlate with the resistance index (RI) on days of hCG administration, follicular aspiration and embryo transfer. However, the concentrations of IFN-α correlated with endometrial thickness on the day of embryo transfer (Spearman correlation coefficient ρ?=?0.4107; P?<?0.05) but not on days of hCG administration and follicular aspiration. The mechanism of VEGF association with the previous ability of having a child needs to be clarified in future studies. The results of this study indicate a possible role of IFN-α in pathways of endometrial remodelling. 相似文献
458.
Orsolya Kiss Inês Catry Jesús M. Avilés Sanja Barišić Tatiana Kuzmenko Svilen Cheshmedzhiev 《Bird Study》2020,67(2):143-159
ABSTRACT Capsule: The distribution range of the European Roller Coracias garrulus has undergone large changes over geological times, but although the species is warm-adapted, the human induced climate change is predicted to affect negatively the range of the currently large populations. Aim: Information on species-specific vulnerability to climate change is crucial not only for designing interventions and setting conservation goals, but also to inform conservation decision-making. Our goal was to map climate suitability for the European Roller in the Western Palaearctic under current climate, and for past (last glacial maximum and mid-Holocene) and future (2050 and 2070) climate scenarios. Methods: We used MaxEnt for species distribution modelling based on the reconstructed distribution map of the species. Results: Our results suggest that during glacial periods Rollers persisted in small southern refugia, and then spread and colonized northern latitudes during the mid-Holocene. In the future, our models forecast a shift in climatically suitable range towards northern latitudes and an overall small range contraction (4.5–5.5%). Warmer temperatures will increase climate suitability in northern countries where the species is currently declining or became locally extinct. On the other hand, wide suitable areas under current climatic conditions are predicted to become unsuitable in the future (35–38% by 2050 and 2070, respectively), significantly impacting large populations such as those in Romania, Spain, Bulgaria and Hungary. French and Italian populations are identified to be future key populations for Roller conservation. Conclusions: Our findings suggest that future climate changes will likely amplify the impacts of existing threats on the majority of large European Roller populations in Europe. 相似文献
459.
Ivana Moric Sanja Bajkic Miloje Savic Tatjana Ilic Tomic Graeme L. Conn Branka Vasiljevic 《The protein journal》2009,28(7-8):326-332
The mechanism of resistance to aminoglycosides based on methylation of their target, 16S rRNA, was until recently described only in antibiotic producing microorganisms. However, equivalent methyltransferases have now also been identified among numerous clinical Gram-negative pathogenic isolates. We have cloned, expressed, and purified GrmA, the aminoglycoside-resistance methyltransferase from Micromonospora purpurea, producer of gentamicin complex. Two vectors were created that express protein with an N-terminal 6× histidine tag with and without an enterokinase recognition producing proteins His6-EK-GrmA and His6-GrmA, respectively. The activity of both recombinant proteins was demonstrated in vivo. After optimized expression and native purification both protein variants proved to be active in in vitro methylation assays. This work lays a foundation for future detailed biochemical, structural and pharmacological studies with this member of an important group of aminoglycoside-resistance enzymes. 相似文献