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21.
Sabir F Sangwan NS Chaurasiya ND Misra LN Sangwan RS 《Zeitschrift für Naturforschung. C, Journal of biosciences》2008,63(5-6):409-412
In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at lower levels. 相似文献
22.
Mandal C Dutta A Mallick A Chandra S Misra L Sangwan RS Mandal C 《Apoptosis : an international journal on programmed cell death》2008,13(12):1450-1464
Withaferin A (WA) is present abundantly in Withania somnifera, a well-known Indian medicinal plant. Here we demonstrate how WA exhibits a strong growth-inhibitory effect on several human
leukemic cell lines and on primary cells from patients with lymphoblastic and myeloid leukemia in a dose-dependent manner,
showing no toxicity on normal human lymphocytes and primitive hematopoietic progenitor cells. WA-mediated decrease in cell viability was observed through apoptosis as demonstrated by externalization of phosphatidylserine,
a time-dependent increase in Bax/Bcl-2 ratio; loss of mitochondrial transmembrane potential, cytochrome c release, caspases 9 and 3 activation; and accumulation of cells in sub-G0 region based on DNA fragmentation. A search for
the downstream pathway further reveals that WA-induced apoptosis was mediated by an increase in phosphorylated p38MAPK expression,
which further activated downstream signaling by phosphorylating ATF-2 and HSP27 in leukemic cells. The RNA interference of
p38MAPK protected these cells from WA-induced apoptosis. The RNAi knockdown of p38MAPK inhibited active phosphorylation of
p38MAPK, Bax expression, activation of caspase 3 and increase in Annexin V positivity. Altogether, these findings suggest
that p38MAPK in leukemic cells promotes WA-induced apoptosis. WA caused increased levels of Bax in response to MAPK signaling,
which resulted in the initiation of mitochondrial death cascade, and therefore it holds promise as a new, alternative, inexpensive
chemotherapeutic agent for the treatment of patients with leukemia of both lymphoid and myeloid origin.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
23.
Mahesh Chandra Alok Kalra Pradeep K. Sharma Rajender S. Sangwan 《Journal of industrial microbiology & biotechnology》2009,36(4):605-609
Capabilities of cellulase production, using delignified bioprocessings of medicinal and aromatic plants, viz. citronella (Cymbopogon winterianus) and Artemisia annua (known as marc of Artemisia) and garden waste (chiefly containing Cynodon dactylon), by the six species of Trichoderma were comparatively evaluated. Among the members of Trichoderma studied, T. citrinoviride was found to be the most efficient producer of cellulases along with a high level of β- glucosidase (produced 102.4 IU g−1 on marc of Artemisia; 101.33 IU g−1 on garden waste; 81.86 IU g−1 on distillation waste of citronella and 94.77 IU g−1 on pure cellulose). Although T. virens was noticed to be the minimal enzyme producer fungus, it interestingly could not produce complete cellulase enzyme complex
on any test waste or pure cellulose, except on marc of Artemisia, where it produced all three enzymes of the complex. Immediate reduction in pH was also noticed during fermentation in the
case of pure polymer (cellulose) by all tested fungi, while it was delayed with delignified agrowastes. The pH profile varied
with the substrate used as well as with individual species of Trichoderma. On the other hand, no alteration in pH with any species of Trichoderma was noticed when grown on marc of A. annua, which might be due to the buffering capacity of this marc. 相似文献
24.
Archana Mathur Ajay Kumar Mathur Anita Gangwar Sharawan Yadav Priyanka Verma Rajender Singh Sangwan 《In vitro cellular & developmental biology. Plant》2010,46(1):13-21
A root-derived callus line of Panax sikkimensis that stably accumulates anthocyanins was established by small cell aggregate selection method. The selected line showed a
growth index of 221.36 and an anthocyanin content of 2.76 mg/g fw (7.076% dw) in 50–60 d of growth on a modified MS medium
containing 4.5 μM 2,4-dichlorophenoxy acetic acid and 1.2 μM kinetin under 16-h light and 8-h dark photoperiodic conditions.
Incubation under continuous light increased the growth index to 435.57 but led to a marginal dilution of anthocyanin content
to 2.192 mg/g fw (6.928% dw). The purple-red pigment had absorption maximum at 528 nm. The selected callus line has shown
sustained growth and productivity for more than 6 yr now. Interestingly, pigment accumulation in the selected line did not
hinder the ginsenoside production in the callus tissue (0.9–1.2% fw). 相似文献
25.
Sean M. Gibbons Tara Schwartz Jennifer Fouquier Michelle Mitchell Naseer Sangwan Jack A. Gilbert Scott T. Kelley 《Applied and environmental microbiology》2015,81(2):765-773
Human-associated bacteria dominate the built environment (BE). Following decontamination of floors, toilet seats, and soap dispensers in four public restrooms, in situ bacterial communities were characterized hourly, daily, and weekly to determine their successional ecology. The viability of cultivable bacteria, following the removal of dispersal agents (humans), was also assessed hourly. A late-successional community developed within 5 to 8 h on restroom floors and showed remarkable stability over weeks to months. Despite late-successional dominance by skin- and outdoor-associated bacteria, the most ubiquitous organisms were predominantly gut-associated taxa, which persisted following exclusion of humans. Staphylococcus represented the majority of the cultivable community, even after several hours of human exclusion. Methicillin-resistant Staphylococcus aureus (MRSA)-associated virulence genes were found on floors but were not present in assembled Staphylococcus pan-genomes. Viral abundances, which were predominantly enterophages, human papilloma virus, and herpesviruses, were significantly correlated with bacterial abundances and showed an unexpectedly low virus-to-bacterium ratio in surface-associated samples, suggesting that bacterial hosts are mostly dormant on BE surfaces. 相似文献
26.
Varnier AL Mazeyrat-Gourbeyre F Sangwan RS Clément C 《Journal of structural biology》2005,152(2):118-128
To characterize the spatial and temporal occurrence of programmed cell death (PCD) in Lilium anther tissues, we used both microscopical and molecular markers of apoptosis for developmental stages from meiosis to pollen release. The first hallmarks of PCD include cell condensation and shrinkage of the cytoplasm, separation of chromatin into delineated masses, and DNA fragmentation in the tapetum as early as the premeiosis stage. PCD then extended to other anther sporophytic tissues, leading to anther dehiscence. Although the PCD clearly affected the endothecium and the epidermis, these two cell layers remained alive until anther dehiscence. In pollen, no sign of PCD was found until pollen mitosis I, after what apoptotic features developed progressively in the vegetative cell. In addition, DNA ladders were detected in all sporophytic tissues and cell types throughout pollen development, whereas in the male gametophyte DNA ladders were only detected during pollen maturation. Our data suggest that PCD is a progressive and active process affecting all the anther tissues, first being triggered in the tapetum. 相似文献
27.
S Anand N Sangwan P Lata J Kaur A Dua AK Singh M Verma J Kaur JP Khurana P Khurana S Mathur R Lal 《Journal of bacteriology》2012,194(16):4471-4472
Sphingobium indicum B90A, an efficient degrader of hexachlorocyclohexane (HCH) isomers, was isolated in 1990 from sugarcane rhizosphere soil in Cuttack, India. Here we report the draft genome sequence of this bacterium, which has now become a model system for understanding the genetics, biochemistry, and physiology of HCH degradation. 相似文献
28.
Summary A stabilized two-year old suspension of a Datura innoxia cell line, producing small amounts of tropane alkaloids (scopolamine and hyoscyamine) was used in this study. Calcium alginate immobilization has been shown to be able to increase secondary metabolite (i. e. alkaloid) production. The effects of calcium and ungellified alginate were both beneficial for tropane alkaloid synthesis; a 10mM calcium chloride supply gave the best results, with a 10-fold yield increase.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- S
standard cell culture medium
- HPLC
high performance liquid chromatography
- FW
fresh weight
- FDA
fluorescein diacetate
- FW
fresh weight 相似文献
29.
30.
Mohamed Abou El Hassan Katherine Huang Manoja B. K. Eswara Zhaodong Xu Tao Yu Arthur Aubry Zuyao Ni Izzy Livne-bar Monika Sangwan Mohamad Ahmad Rod Bremner 《BMC molecular biology》2017,18(1):6