全文获取类型
收费全文 | 16787篇 |
免费 | 1301篇 |
国内免费 | 587篇 |
专业分类
18675篇 |
出版年
2024年 | 28篇 |
2023年 | 145篇 |
2022年 | 359篇 |
2021年 | 559篇 |
2020年 | 410篇 |
2019年 | 526篇 |
2018年 | 596篇 |
2017年 | 491篇 |
2016年 | 686篇 |
2015年 | 1033篇 |
2014年 | 1125篇 |
2013年 | 1300篇 |
2012年 | 1545篇 |
2011年 | 1364篇 |
2010年 | 891篇 |
2009年 | 771篇 |
2008年 | 950篇 |
2007年 | 853篇 |
2006年 | 724篇 |
2005年 | 632篇 |
2004年 | 588篇 |
2003年 | 504篇 |
2002年 | 457篇 |
2001年 | 254篇 |
2000年 | 250篇 |
1999年 | 237篇 |
1998年 | 120篇 |
1997年 | 132篇 |
1996年 | 117篇 |
1995年 | 96篇 |
1994年 | 90篇 |
1993年 | 83篇 |
1992年 | 106篇 |
1991年 | 111篇 |
1990年 | 73篇 |
1989年 | 61篇 |
1988年 | 56篇 |
1987年 | 56篇 |
1986年 | 42篇 |
1985年 | 43篇 |
1984年 | 27篇 |
1983年 | 26篇 |
1982年 | 12篇 |
1981年 | 17篇 |
1979年 | 18篇 |
1978年 | 20篇 |
1975年 | 12篇 |
1974年 | 10篇 |
1972年 | 13篇 |
1971年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
11.
Suárez-Cortez R Alvarez-Gallegos J González-Mora E 《Biotechnology and bioengineering》1989,33(4):377-385
The design of a sliding controller for a continuous fermentation process is presented. The results obtained by simulation have proved the control scheme to be very robust. Regulation of substrate concentration at its optimal value has been achieved even though process parameters change their nominal values. Chattering effects are decreased by introducing a minor modification of the control variable around the sliding surface. 相似文献
12.
Su Shaozhi 《Dialectical Anthropology》1991,16(3-4):301-307
Conclusion To sum up, the character of China's existing system is basically all-embracing totalitarianism formed by the combination of the old tradition of feudal-despotism and the new tradition of Stalinism. This system is the basic source of all the social evils in China.The ultimate aim of the democratic movement is to transform the old, allembracing totalitarian system and to establish a democratic, free, humane, and rational new system. The present ruling group is the beneficiary and defender of the existing system. The contradictions and conflicts between reformers who want to transform the old system and the hardliners who want to protect the old system formed the crux of the struggle of the 1989 democratic movement. Because the democratic movement lacked preparation in theory, organization, tactics and strategy, and because the ruling group controls the means of dictatorship — especially the army —military force was used to suppress the democratic movement with brutal bloodshed. In order to promote the democratic movement, we should sum up some lessons and experiences. First, we should study in depth the existing system and oppose feudaldespotism and Stalinism through effective propaganda and education. Second, we should arouse the democratic consciousness of the broad masses of the people, uniting with them and the healthy elements inside the Party, government, and army to struggle for the smashing of the old system and the establishment of a new system.Su Shaozhi is former Director of the Institute for the Study of Marxism-Leninism and Mao Zedong Thought, Chinese Academy of Social Sciences, Beijing, and currently Visiting Professor at the Bradley Institute for Democracy and Public Values, Marquette University, Milwaukee, Wisconsin 相似文献
13.
Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies 总被引:6,自引:0,他引:6
Chun-Ming Huang Marilyn Parsons Vernon T. Oi Huei-Jen Su Huang Leonard A. Herzenberg 《Immunogenetics》1983,18(4):311-321
We have generated a new series of monoclonal antibodies recognizing allotypic determinants on mouse IgG1, IgG2a, and IgG2b. In this communication we describe their reactivities with immunoglobulins of the inbred mouse strains. Comparison with serology charts indicates that many of these monoclonal antibodies detect allotypic specificities previously defined by conventional antisera; others define previously undescribed specificities. Strain and isotype distribution allows us to assign five new allotypic specificities to Igh-1 and three new specificities to Igh-3. In addition, on the basis of reactivity with the monoclonal antibodies, we have defined a new Igh haplotype in SWR/J mice, Igh
p.Abbreviations used in this paper Igh
immunoglobulin heavy chain
- SDS
sodium dodecyl sulfate 相似文献
14.
15.
Isolation and characterization of the Escherichia coli mutL gene product 总被引:33,自引:0,他引:33
The Escherichia coli mutL gene product has been purified to near homogeneity from an overproducing clone. The mutL locus encodes a polypeptide of 70,000 daltons as determined by denaturing gel electrophoresis. The native molecular weight of MutL protein as calculated from the sedimentation coefficient of 5.5 S and Stokes radius of 61 A is 139,000 daltons, indicating that MutL exists as a dimer in solution. In addition to its ability to complement methyl-directed DNA mismatch repair in mutL-deficient cell-free extracts, DNase I protection experiments demonstrate that the purified MutL protein interacts with the MutS-heteroduplex DNA complex in the presence of ATP. 相似文献
16.
C K Chou T S Su C M Chang C P Hu M Y Huang C S Suen N W Chou L P Ting 《The Journal of biological chemistry》1989,264(26):15304-15308
The human hepatoma Hep3B cells contain integrated hepatitis B viral genome and continually secret hepatitis B surface antigen (HBsAg). The production of HBsAg (but not alpha-fetoprotein) was suppressed by addition of low concentrations (0.1-1 nM) of insulin into serum-free medium. In addition, the suppression of HBsAg production by insulin was paralleled with the decrease in HBsAg mRNA abundance. Insulin also cause a rapid rate of disappearance of HBsAg mRNA (t 1/2, 2 h) in Hep3B cells. The Hep3B cells carry specific receptor with high affinity for insulin (Kd = 1.8 nM). The receptor showed an insulin-dependent protein tyrosine kinase activity. The half-maximal insulin concentration for the activation of the receptor kinase was about 5 nM. Only very high concentrations of insulin-like growth factor I and human proinsulin can compete for the insulin receptor binding and suppress HBsAg production, this suggests that insulin may act through its receptor binding to suppress HBsAg expression in human hepatoma Hep3B cells. 相似文献
17.
I Grys M Dudziak W Piechota W Rózański M Su?ek M Adamczyk-Szymańska 《Polski tygodnik lekarski (Warsaw, Poland : 1960)》1990,45(49-50):984-986
The study involved 126 patients with cholelithiasis. Set of biochemical, radiological, endoscopic, and ultrasound examinations was carried out in these patients. Potential coincidence of clinical symptoms and causes of so-called postcholecystectomy syndrome was the purpose of a 1-year follow-up studies. The significance of the results are discussed. 相似文献
18.
Computational sequence analysis of matrix metalloproteinases 总被引:12,自引:0,他引:12
Matrix metalloproteinases (MMP) play a cardinal role in the breakdown of extracellular matrix involved in a variety of biological and pathological processes. Research on MMPs has classified and characterized these enzymes according to their matrix substrate specificity, gene and protein domain structure, and regulation of activity and expression. However, the discovery of new MMPs has introduced a need for a more comprehensive and systematic method of classification and quantitative comparison of known and newly discovered members. This study compiles a sequence alignment, constructs a dendrogram, and calculates physical data and homology percentage assignments in order to obtain further insight into MMP structure-function relationships. Thorough analysis of MMP primary sequence domains, physical data patterns, and statistical analysis of sequence homology yields higher resolution in the similarities and differences that group MMP members. 相似文献
19.
Summary Cell growth increased proportionally to the initial concentration of ammonium ion, however, methylan production was significantly inhibited at the high concentration of ammonium ion. The control of ammonium ion within the desired level(usually 0.45 g/l) was needed to reduce the inhibition. Methylan production was increased to 12.5 g/l by maintaining ammonium ion below 0.15 g/l. 相似文献
20.
Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum. 下载免费PDF全文
Upon induction with heparin, Flavobacterium heparinum synthesizes and secretes into its periplasmic space heparinase I (EC 4.2.2.7), heparinase II, and heparinase III (heparitinase; EC 4.2.2.8). Heparinase I degrades heparin, and heparinase II degrades both heparin and heparan sulfate, while heparinase III degrades heparan sulfate predominantly. We isolated the genes encoding heparinases II and III (designated hepB and hepC, respectively). These genes are not contiguous with each other or with the heparinase I gene (designated hepA). hepB and hepC were found to contain open reading frames of 2,316 and 1,980 bp, respectively. Enzymatic removal of pyroglutamate groups permitted sequence analysis of the amino termini of both mature proteins. It was determined that the mature forms of heparinases II and III contain 746 and 635 amino acids, respectively, and have calculated molecular weights of 84,545 and 73,135, respectively. The preproteins have signal sequences consisting of 26 and 25 amino acids. Truncated hepB and hepC genes were used to produce active, mature heparinases II and III in the cytoplasm of Escherichia coli. When these enzymes were expressed at 37 degrees C, most of each recombinant enzyme was insoluble, and most of the heparinase III protein was degraded. When the two enzymes were expressed at 25 degrees C, they were both present predominantly in a soluble, active form. 相似文献