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991.
992.
Comparative biometry and isotopy of three dominant pennatulacean corals in the Northwest Atlantic
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Sandrine Baillon Matthew English Jean‐François Hamel Annie Mercier 《Acta zoologica》2016,97(4):475-493
Spatial and temporal shifts in biometric features were examined in three common deep‐water pennatulacean corals (sea pens) from the Northwest Atlantic: Anthoptilum grandiflorum, Halipteris finmarchica and Pennatula aculeata. These three species show different morphological characters and adaptations to their environment. Analyses of colony length, ratio of peduncle to colony length, weight/length ratio, polyp size and density as well as sclerite shape, location and abundance indicate that their phenotype is modulated by environmental factors (e.g. food availability, currents and sediment type) and antipredator strategies. Moreover, the three species had different carbon and nitrogen stable isotope signatures that could be explain primarily by their different polyp diameters and colony shapes, suggesting that they rely on slightly different food sources (varying proportions of phytodetritus and zooplankton). Finally, sclerites were found only in H. finmarchica and P. aculeata and are not known to occur in A. grandiflorum, except for minute oval bodies inside the peduncle. The Mg/Ca ratio of sclerites differed between the two species and, for P. aculeata, among types of sclerites, evoking different biomineralization pathways related to their functional roles (structural support or defence). Overall, this study provides new information on the ecology of poorly known species, which are ubiquitous and suspected to play an important ecological role in deep‐water ecosystems. 相似文献
993.
Atopic dermatitis induces the expansion of thymus‐derived regulatory T cells exhibiting a Th2‐like phenotype in mice
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Verena Moosbrugger‐Martinz Christoph H. Tripp Björn E. Clausen Matthias Schmuth Sandrine Dubrac 《Journal of cellular and molecular medicine》2016,20(5):930-938
Atopic dermatitis (AD) is a widespread inflammatory skin disease with an early onset, characterized by pruritus, eczematous lesions and skin dryness. This chronic relapsing disease is believed to be primarily a result of a defective epidermal barrier function associated with genetic susceptibility, immune hyper‐responsiveness of the skin and environmental factors. Although the important role of abnormal immune reactivity in the pathogenesis of AD is widely accepted, the role of regulatory T cells (Tregs) remains elusive. We found that the Treg population is expanded in a mouse model of AD, i.e. mice topically treated with vitamin D3 (VitD). Moreover, mice with AD‐like symptoms exhibit increased inducible T‐cell costimulator (ICOS)‐, cytotoxic T‐lymphocyte antigen‐4 (CTLA‐4)‐ and Glycoprotein‐A repetitions predominant receptor (GARP)‐expressing Tregs in skin‐draining lymph nodes. Importantly, the differentiation of Tregs into thymus‐derived Tregs is favoured in our mouse model of AD. Emigrated skin‐derived dendritic cells are required for Treg induction and Langerhans cells are responsible for the biased expansion of thymus‐derived Tregs. Intriguingly, thymus‐derived Tregs isolated from mice with AD‐like symptoms exhibit a Th2 cytokine profile. Thus, AD might favour the expansion of pathogenic Tregs able to produce Th2 cytokines and to promote the disease instead of alleviating symptoms. 相似文献
994.
Identification of class II ADP‐ribosylation factors as cellular factors required for hepatitis C virus replication
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Rayan Farhat Karin Séron Juliette Ferlin Lucie Fénéant Sandrine Belouzard Lucie Goueslain Catherine L. Jackson Jean Dubuisson Yves Rouillé 《Cellular microbiology》2016,18(8):1121-1133
GBF1 is a host factor required for hepatitis C virus (HCV) replication. GBF1 functions as a guanine nucleotide exchange factor for G‐proteins of the Arf family, which regulate membrane dynamics in the early secretory pathway and the metabolism of cytoplasmic lipid droplets. Here we established that the Arf‐guanine nucleotide exchange factor activity of GBF1 is critical for its function in HCV replication, indicating that it promotes viral replication by activating one or more Arf family members. Arf involvement was confirmed with the use of two dominant negative Arf1 mutants. However, siRNA‐mediated depletion of Arf1, Arf3 (class I Arfs), Arf4 or Arf5 (class II Arfs), which potentially interact with GBF1, did not significantly inhibit HCV infection. In contrast, the simultaneous depletion of both Arf4 and Arf5, but not of any other Arf pair, imposed a significant inhibition of HCV infection. Interestingly, the simultaneous depletion of both Arf4 and Arf5 had no impact on the activity of the secretory pathway and induced a compaction of the Golgi and an accumulation of lipid droplets. A similar phenotype of lipid droplet accumulation was also observed when GBF1 was inhibited by brefeldin A. In contrast, the simultaneous depletion of both Arf1 and Arf4 resulted in secretion inhibition and Golgi scattering, two actions reminiscent of GBF1 inhibition. We conclude that GBF1 could regulate different metabolic pathways through the activation of different pairs of Arf proteins. 相似文献
995.
996.
Common species have lower taxonomic diversity Evidence from the urban floras of Brussels and Rome 总被引:1,自引:0,他引:1
The species pool hypothesis claims that the large‐scale regional species pool is the chief parameter in determining small‐scale species richness through filtering of species that can persist within a community on the basis of their tolerance of the abiotic environment. Accordingly, different environmental conditions give rise to different species assemblages. From a taxonomic perspective, under the assumption of trait conservatism, co‐occurring species that experience similar environmental conditions are likely to be more taxonomically similar than ecologically distant species. The next step consists in understanding how commonness and rarity of individual species produce the observed taxonomic diversity. In this paper, the importance of environmental filtering in regulating the taxonomic structure of rare and common plant species in the urban floras of Brussels (Belgium) and Rome (Italy) is tested. First, we computed the taxonomic diversity of the rare and common species of Brussels and Rome based on the branching topology of the Linnaean taxonomic trees. Next, using a randomization procedure, we determined whether the taxonomic diversity of the rare species was significantly higher than the diversity of the common species. Results show that, for both urban floras, common species that shape the community matrix and experience similar environmental conditions have a taxonomic diversity that is significantly lower than that of the rare species that represent a relatively incidental set of species of more ‘disperse’ origin. Finally, from a conservation/management perspective our results imply that, given their high taxonomic heterogeneity, the protection of rare species is a central issue for preserving high levels of diversity in urban areas. 相似文献
997.
998.
Adrian L. D. Paul Sandrine Isnard Christine M. Wawryk Peter D. Erskine Guillaume Echevarria Alan J. M. Baker Jason K. Kirby Antony van der Ent 《The Plant journal : for cell and molecular biology》2021,107(4):1040-1055
The hyperaccumulator Pycnandra acuminata is a New Caledonian rainforest tree known to have the highest concentration of nickel in any living organism, with 25 wt% nickel in its latex. All trees (with a diameter of >10 cm) and soil profiles in a 0.25-hectare permanent plot were sampled to assess the biogeochemical compartmentalisation of nickel in a dense stand of P. acuminata trees. Nickel stable isotope analysis permitted insights into the cycling of nickel in this ecosystem. The total tree biomass of the plot was calculated to be 281 tonnes ha−1, which contained 0.44 kg of cobalt, 49.1 kg of manganese, 257 kg of nickel and 6.76 kg of zinc. Nickel stable isotope analysis identified the biotic origin of the nickel in the soil upper layers, with P. acuminata shoots enriched in lighter nickel isotopes. The δ60Ni latex signature suggests that long-distance transport, radial xylem and phloem loading are at play in P. acuminata. 相似文献
999.
1000.
Eric Grouzmann Jean‐Baptiste Gualtierotti Sandrine Gerber‐Lemaire Karim Abid Noureddine Brakch Alessandro Pedretti Bernard Testa Giulio Vistoli 《Chirality》2013,25(1):28-34
(1R)‐Normetanephrine is the natural stereoisomeric substrate for sulfotransferase 1A3 (SULT1A3)‐catalyzed sulfonation. Nothing appears known on the enantioselectivity of the reaction despite its potential significance in the metabolism of adrenergic amines and in clinical biochemistry. We confronted the kinetic parameters of the sulfoconjugation of synthetic (1R)‐normetanephrine and (1S)‐normetanephrine by recombinant human SULT1A3 to a docking model of each normetanephrine enantiomer with SULT1A3 and the 3′‐phosphoadenosine‐5′‐phosphosulfate cofactor on the basis of molecular modeling and molecular dynamics simulations of the stability of the complexes. The KM, Vmax, and kcat values for the sulfonation of (1R)‐normetanephrine, (1S)‐normetanephrine, and racemic normetanephrine were similar. In silico models were consistent with these findings as they showed that the binding modes of the two enantiomers were almost identical. In conclusion, SULT1A3 is not substrate‐enantioselective toward normetanephrine, an unexpected finding explainable by a mutual adaptability between the ligands and SULT1A3 through an “induced‐fit model” in the catalytic pocket. Chirality, 25:28‐34, 2012.© 2012 Wiley Periodicals, Inc. 相似文献