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241.
Mitochondrial Ribosomes in HeLa Cells 总被引:5,自引:0,他引:5
HeLa cell mitochondria contain 60S ribosomes which seem to consist of subunits of 45S and 35S particles. The 16S and 12S RNA components are coded by mitochondrial DNA. 相似文献
242.
TRANSFORMATION of allogenic lymphocytes in mixed cultures depends chiefly on an incompatibility between the lymphocyte donors at the major histocompatibility locus in man (HL-A), mouse (H-2) and rat (H-l)1. Although the mouse H-2 locus can be divided into several regions each of which controls one or more antigenic specificities2 and two or more subloci control HL-A antigens in man3, it is not known whether all parts of the major histocompatibility locus are equally important in eliciting transformation in mixed lymphocyte cultures. We now show that capacity to elicit lymphocyte transformation is different for different parts of the mouse H-2 locus. 相似文献
243.
Demonstration of an “Excitation-Contraction Recoupling” Mechanism in Mammalian Ventricular Myocardium 总被引:1,自引:0,他引:1
A PROCESS called “excitation-contraction coupling” has been generally accepted to take place only in the direction of excitation to contraction. Through this mechanism a propagated action potential initiates an active state in skeletal or cardiac muscle and the muscle contracts. We propose that, in the mammalian ventricular myocardium at least, the process is not unidirectional and an important reverse coupling between the contractile system and the excitable plasma membrane has been overlooked. Through this feedback interaction the mode of contraction (that is, isotonic or isometric) not only determines the instantaneous electrical state of the plasma membrane, but also influences the mechanical events of the subsequent beats. Thus when Kaufmann et al.1 recorded intracellular action potentials from cat papillary muscle, the time course of the repolarization was altered depending on the mode of contraction. Some kind of contraction-excitation feedback has also been suggested by Stauch2 and Lab3,4. They showed a difference in the shape of the monophasic action potential, as recorded by a suction electrode, when comparing isotonic and isovolumic contraction of the intact ventricle. But their experimental conditions did not allow satisfactory analysis of the phenomenon. 相似文献
244.
The Structure of Calf Liver Cytochrome <Emphasis Type="Italic">b</Emphasis><Subscript>5</Subscript> at 2.8 Å Resolution 总被引:5,自引:0,他引:5
CYTOCHROME b5 is a haem-containing protein in the microsomes of liver tissue. It interacts specifically with a flavo-protein, cytochrome b5 reductase, which catalyses the transfer of electrons from NADH to the haem iron of the cytochrome1. The microsomal cytochrome b5 system has been implicated in fatty acid desaturation reactions2 and a similar system in erythrocytes may catalyse the reduction of methaemoglobin3. Calf liver cytochrome b5, solubilized by pancreatic lipase, has a molecular weight of 11,000 and consists of ninety-three amino-acids in the sequence shown in Fig. 1 (refs. 4 and 5). The haem group is non-covalently bound to the protein and can be removed reversibly by acid acetone treatment6. 相似文献
245.
KOJI NAKANISHI STEVEN BLOBSTEIN MAKOTO FUNAMIZU NOBUO FURUTACHI GEORGE VAN LEAR DEZIDER GRUNBERGER KARL W. LANKS I. BERNARD WEINSTEIN 《Nature: New biology》1971,234(47):107-109
WE wish to report that reconstituted sperm whale myoglobin prepared by the method of Breslow1 (except that pH 2 was found sufficient to remove all the haem) (I) crystallizes2 in a different habit from those prepared by the method of Rossi-Fanelli et al.3 (II) using haemin of Sigma lot 77B-0220 and our own 57Fe photoporphyrin preparation and the native myoglobin (III). Although all three form type A3 monoclinic prisms, the best developed plane is [001] for II and III, it is [100] for I. There seems to be great interest in reconstituted haemoproteins4,5, so it is important that crystallization habit may be a sensitive test for subtle changes in protein structures. 相似文献
246.
Role of the Galactose Binding Protein in Chemotaxis of <Emphasis Type="Italic">Escherichia coli</Emphasis> toward Galactose 总被引:51,自引:0,他引:51
The galactose binding protein is the part of the galactose chemoreceptor which recognizes the attractants galactose, glucose and a number of structurally related chemicals. 相似文献
247.
Ribosomes,G-factor and Siomycin 总被引:13,自引:0,他引:13
G-factor interacts with the 50S ribo-somal subunit at a site which is distinct from the peptidyl transferase centre and which is inactivated by siomycin. 相似文献
248.
Passive Haemagglutination Test for Anti-rhinovirus Antibodies 总被引:2,自引:0,他引:2
W. PAGE FAULK G. N. VYAS C. A. PHILLIPS H. HUGH FUDENBERG K. CHISM 《Nature: New biology》1971,231(21):101-104
The use of chromic chloride as a coupling reagent has made it possible to coat red cells with rhinovirus protein. This is shown by immunofluorescence, electron microscopy and immunocolloidal experiments. 相似文献
249.
RICHARD L. SIMMONS MARTIN L. LIPSCHULTZ ANGELYN RIOS PRASANTA K. RAY 《Nature: New biology》1971,231(21):111-112
DURING outbred pregnancy the mother is exposed to genetically foreign tissue because the offspring inherits transplantation antigens from the father. The survival of the foetus is ensured by the intervention of the trophoblast which does not express transplantation antigens between mother and foetus: mouse trophoblast is not rejected even when transplanted into immune recipients1,3. The mechanism of this failure to express histocompatibility antigens is not understood1–4, but Kirby et al. have suggested that the extracellular fibrinoid surrounding trophoblast cells is involved5,6. Currie has suggested that the thick sialomucinous glycocalyx of the trophoblast cell might “mask” the histocompatibility antigens on the trophoblast7,8 and has demonstrated that neuraminidase unmasked these antigens8. Our experiments, however, show that trophoblast incubated with neuraminidase cannot sensitize allogeneic mice to donor histocompatibility antigens. Furthermore, pretreatment of trophoblastic implants with neuraminidase did not interfere with their proliferation and growth in highly immune allogeneic recipients. 相似文献
250.
Constant Production of Type C Virus Particles in a Continuous Tissue Culture derived from Pleural Effusion Cells of a Lymphoma Patient 总被引:5,自引:0,他引:5
ELIZABETH S. PRIORI LEON DMOCHOWSKI BROOKS MYERS J. R. WILBUR 《Nature: New biology》1971,232(28):61-62
PARTICLES resembling viruses were first observed in organs of mice with spontaneous leukaemia and in thin sections of a biopsy specimen from a lymph node of a patient with acute lymphocytic leukaemia by Dmochowski and Grey1. They also found them in some biopsy specimens of lymph nodes and bone marrows before and after growth in tissue culture2,3,8. Recent reports have described the presence of type C virus particles in other human tumours including liposarcoma4, osteosarcoma5,6, giant cell tumour9, rhabdomyosarcoma (unpublished results of L. D. and E. S. P.) and breast carcinoma7. 相似文献