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81.
C G Zamorano M C Contreras A Sánchez M A Bahamondes L Sandoval 《Boletín chileno de parasitología》1991,46(3-4):82-84
San Juan de la Costa County (40 degrees 45' South lat., 73 degrees 19' West long.) is located in the Osorno province, South of Chile. Its population is 8,486 inhabitants. The basic economic activities are agriculture, cattle raising, timber production and manufacture of wood and coal. According to official reports, the incidence of human hydatidosis and trichinosis in this locality in 1989 were 24 and 59 per 100,000 respectively. In order to contribute to a better knowledge of the epidemiology of human hydatidosis and trichinosis in San Juan de la Costa County, an indirect hemagglutination test (IHAT) for these parasitoses was performed to 511 randomized people. Nine (1.8%) individuals resulted positive for hydatidosis and twenty four (4.7%) were positive for trichinosis. Some considerations on the corresponding prophylactic measures are proposed. 相似文献
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The experiments were conducted to explore the possibility that formic acid could serve as a reducing agent for carbonyl compounds on the prebiotic earth, since it has been shown that formic acid is a product of numerous chemical evolution experiments. The photoreduction of solutions 0.33 M in acetaldehyde or acetone at 2537 Å in 0.167 M formic acid, sodium formate, or both formic acid and sodium formate in a nitrogen atmosphere has been conducted. The formation of the corresponding reduction product, ethanol or isopropyl alcohol, and the disappearance of the reactant were followed as a function of irradiation time by using gas chromatography. Product confirmation was done by NMR analysis and mass spectrometry. 相似文献
83.
Graciela E. Santillán Marisa J. Sandoval Yuti Chernajovsky Patricia L. Orchansky 《Molecular and cellular biochemistry》1992,110(2):181-191
A number of cell-surface proteins are anchored in plasma membranes by a glycosylated phosphatidylinositol (PI) moiety that
is covalently attached to the carboxyl-terminal amino acid of the mature protein. We have previously reported the construction
of a cDNA clone of a truncated Platelet-derived growth factor (PDGF) receptor that consists of the extracellular domain without
the transmembrane and cytoplasmic domains. In the construction of the vector, a sequence of 51 base pairs (bp) from the 3′-untranslated
region of the receptor cDNA was linked in frame with the external domain coding sequence. The truncated receptor protein with
the peptide VTSGHCHEERVDRHDGE fused to its carboxyl terminus was covalently attached to the membrane by a PI linkage and it
was released by phosphatidylinositol specific-phospholipase C (PI-PLC). When the 51 bp sequence was deleted, the external
domain receptor protein was secreted into the media. To determine whether the PI linkage of the protein was due to the 17
amino acids added, the peptide was fused to the carboxyl terminus of the secreted protein human Interferon-β (hu-IFN-β). Chinese
hamster ovary (CHO) cells transfected with the hu-IFN-β cDNA secreted the protein to theconditioned media, whereas CHO cells
transfected with the carboxyl terminus modified-hu-IFN-β cDNA did not secrete detectable levels of protein. CHO cells expressing
the carboxyl terminus modified-hu-IFN-β were treated with PI-PLC, the media and cell lysates were analyzed by SDS-PAGE after
immunoprecipitation with antibodies against hu-IFN-β. The modified protein is anchored to the plasma membrane by a PI linkage
and it is specifically released by PI-PLC, whereas a control preparation of CHO cells expressing wild type hu-IFN-β does not
show the same pattern. The 17 amino acid peptide fused to the carboxyl terminus of IFN-β directs attachment of a PI anchor
and targets the fusion protein to the plasma membrane. 相似文献
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Iván González-Chavarría Rita P. Cerro Natalie P. Parra Felipe A. Sandoval Felipe A. Zu?iga Valeska A. Omazábal Liliana I. Lamperti Silvana P. Jiménez Edelmira A. Fernandez Nicolas A. Gutiérrez Federico S. Rodriguez Sergio A. Onate Oliberto Sánchez Juan C. Vera Jorge R. Toledo 《PloS one》2014,9(8)
Altered expression and function of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) has been associated with several diseases such as endothelial dysfunction, atherosclerosis and obesity. In these pathologies, oxLDL/LOX-1 activates signaling pathways that promote cell proliferation, cell motility and angiogenesis. Recent studies have indicated that olr1 mRNA is over-expressed in stage III and IV of human prostatic adenocarcinomas. However, the function of LOX-1 in prostate cancer angiogenesis remains to be determined. Our aim was to analyze the contribution of oxLDL and LOX-1 to tumor angiogenesis using C4-2 prostate cancer cells. We analyzed the expression of pro-angiogenic molecules and angiogenesis on prostate cancer tumor xenografts, using prostate cancer cell models with overexpression or knockdown of LOX-1 receptor. Our results demonstrate that the activation of LOX-1 using oxLDL increases cell proliferation, and the expression of the pro-angiogenic molecules VEGF, MMP-2, and MMP-9 in a dose-dependent manner. Noticeably, these effects were prevented in the C4-2 prostate cancer model when LOX-1 expression was knocked down. The angiogenic effect of LOX-1 activated with oxLDL was further demonstrated using the aortic ring assay and the xenograft model of tumor growth on chorioallantoic membrane of chicken embryos. Consequently, we propose that LOX-1 activation by oxLDL is an important event that enhances tumor angiogenesis in human prostate cancer cells. 相似文献
89.
Vassiliki Lalioti Silvia Vergarajauregui Ignacio V. Sandoval 《Molecular membrane biology》2013,30(4):257-264
The trafficking of the insulin-sensitive glucose transporter, GLUT4, is the paradigm of how cells control the movement of membrane proteins through intricate pathways of transport in response to external stimuli, and how, by doing so, regulate their function. The GLUT4 intracellularly sequestered in resting adipocytes and muscle cells becomes exposed on their surface in response to an increase in insulin levels and muscle contraction, where it facilitates glucose uptake. Ceasing of the stimuli is followed by endocytosis of the GLUT4 molecules exposed on the plasma membrane and their recycling to the original stores, where they are retained. This review discusses current understanding of the organelles that host GLUT4 and the motifs that mediate its trafficking. 相似文献
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