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131.
132.
Changes in light or water availability can result in synchronous leaf production, concentrating food availability for herbivores of young leaves to only a few months. To determine the importance of food availability on herbivory, leaf phenology and leaf damage were studied in the Luquillo Experimental Forest (LEF) of Puerto Rico. We studied 20 individuals of eight species for two years. Every month, new leaves were marked; the following month, leaf area and area of damage were measured. Over two years, comparison of leaf production and percent herbivory were performed for each species, and for all species taken together. More than 30 percent of the annual leaf production occurred in May and June. Leaf production was associated with an increase in PFD (photon flux density) and was not related to the patterns of rainfall. Although leaf production was synchronous, there were no differences in herbivory between the peak and non‐peak periods of leaf production. Possible explanations for the constant levels of herbivory throughout the year are the presence of a generalist herbivore community, the ability of herbivores to track changes in food availability, or high densities of herbivore predators that control herbivore populations. 相似文献
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M A Vega F Rodriguez B Seguí C Calés J Alcalde I V Sandoval 《The Journal of biological chemistry》1991,266(25):16269-16272
Time course experiments of the localization of rat LIMP II expressed in COS cells show that the protein is transported directly from the Golgi complex to lysosomes. Substitution of the tyrosine-lacking carboxyl cytoplasmic tail of LIMP II for the native cytoplasmic tails of the plasma membrane proteins CD36 and CD8 resulted in straight transport of both proteins to lysosomes. The synthetic tyrosine-containing heptapeptide, RGTGVYG, did not replace the natural carboxyl cytoplasmic tail of LIMP II in its ability to transport both CD36 and CD8 to lysosomes, and the two constructs were transported to and expressed at the plasma membrane. Substitution of the cytoplasmic tails of either CD36 or CD8 for the carboxyl cytoplasmic tail of LIMP II resulted in transport of the mutants to the plasma membrane where they underwent endocytosis before accumulating into lysosomes. The results indicate that a motif contained in the tyrosine-lacking carboxyl cytoplasmic tail of LIMP II is sufficient to target proteins directly from the Golgi complex to lysosomes. 相似文献
137.
M A Vega B Seguí-Real J A García C Calés F Rodríguez J Vanderkerckhove I V Sandoval 《The Journal of biological chemistry》1991,266(25):16818-16824
LIMP II is a glycoprotein expressed in the membrane of lysosomes and secretory granules with lysosomal properties. Sequence analysis of a CNBr-cleaved peptide allowed the synthesis of a 47-mer oligonucleotide that was used to screen a rat liver cDNA library in lambda gt11. This resulted in isolation of a 2-kilobase cDNA containing 1,434 bases encoding the entire protein. The deduced amino acid sequence indicates that LIMP II consists of 478 amino acid residues. The segment spanning residues 4-6 to 26 constitute an uncleavable signal peptide. LIMP II possesses a hydrophobic amino acid segment near the carboxyl end, that together with the uncleaved signal peptide may anchor the protein to the membrane through two distant segments. The major portion of the protein resides on the luminal side and displays 11 potential N-glycosylation sites and 5 cysteine residues. Two short cytoplasmic tails, 2-4 and 20-21 amino acids long, correspond to the NH2- and COOH-terminal ends of the protein, respectively. Transfection of COS cells with the cDNA of LIMP II resulted in expression of the protein and its transport to lysosomes. Comparison of the entire sequence to various data bases of known proteins revealed extensive homology between LIMP II and the cell surface protein CD36 involved in cell adhesion. No significant homology was detected with the two families of lysosomal membrane proteins A and B, recently described. 相似文献
138.
Pseudomonas aeruginosa diaminopimelate decarboxylase: evolutionary relationship with other amino acid decarboxylases 总被引:1,自引:0,他引:1
Martin C; Cami B; Yeh P; Stragier P; Parsot C; Patte JC 《Molecular biology and evolution》1988,5(5):549-559
The lysA gene encodes meso-diaminopimelate (DAP) decarboxylase
(E.C.4.1.1.20), the last enzyme of the lysine biosynthetic pathway in
bacteria. We have determined the nucleotide sequence of the lysA gene from
Pseudomonas aeruginosa. Comparison of the deduced amino acid sequence of
the lysA gene product revealed extensive similarity with the sequences of
the functionally equivalent enzymes from Escherichia coli and
Corynebacterium glutamicum. Even though both P. aeruginosa and E. coli are
Gram-negative bacteria, sequence comparisons indicate a greater similarity
between enzymes of P. aeruginosa and the Gram- positive bacterium C.
glutamicum than between those of P. aeruginosa and E. coli enzymes.
Comparison of DAP decarboxylase with protein sequences present in data
bases revealed that bacterial DAP decarboxylases are homologous to mouse
(Mus musculus) ornithine decarboxylase (E.C.4.1.1.17), the key enzyme in
polyamine biosynthesis in mammals. On the other hand, no similarity was
detected between DAP decarboxylases and other bacterial amino acid
decarboxylases.
相似文献
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Sandoval H. Aguilar A. Paniagua C. Martín J. F. 《Applied microbiology and biotechnology》1984,19(6):409-413
Summary The corynebacteria seem to be the most suitable microorganisms for cloning genes involved in the production of amino acids, nucleotides, and other products of industrial interest. A plasmid, pCCl, from Corynebacterium callunae has been found with a size of 4.3 kb. A physical map obtained with restriction endonucleases is presented. pCCl has single restriction sites for Kpn I, Sma I, Bal I, and Hind III. Copy number of this plasmid has been estimated to be about 30. A number of hybrid plasmids have been constructed between pCCl and pBR329 from Escherichia coli and transformed into corynebacteria. The thiostrepton resistance gene (tsr) from Streptomyces azureus has been inserted into them. 相似文献