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111.
Lalioti VS Vergarajauregui S Tsuchiya Y Hernandez-Tiedra S Sandoval IV 《Journal of cellular physiology》2009,218(2):416-426
We have previously reported the physical interaction between Daxx, the adaptor protein that mediates activation of the Jun amino-terminal kinase (JNK), and GLUT4, the insulin-dependent glucose transporter, interaction that involves their C-domains. Co-immunoprecipitation and two-hybrid-based protein-protein interaction studies show now that Daxx and GLUT4 interact with JNK1 through D-sites in their NH(2)-(aa 1-501) and large endofacial loop, respectively. Serum deprivation strongly enhances the association of JNK1 with Daxx and dissociates the kinase from GLUT4. SP600125, a potent JNK1 inhibitor, reduces the JNK1 activity associated with GLUT4 and the phosphorylation of two minor GLUT4 species in serum-starved 3T3-L1 adipocytes. In addition, Daxx interacts with kinesin KIF5B through the 6xTPR domain of the kinesin light chain, a domain engaged in the grab hold of protein cargo by kinesin motors that codistribute with JNK. Depletion of Daxx in 3T3-L1 adipocytes provokes the partial translocation of the GLUT4 retained in the GLUT4 storage compartment to endosomes. 相似文献
112.
113.
Kevin D. Lafferty Darcie Goodman Cristina P. Sandoval 《Biodiversity and Conservation》2006,15(7):2217-2230
Promoting recreation and preserving wildlife are often dual missions for land managers, yet recreation may impact wildlife.
Because individual disturbances are seemingly inconsequential, it is difficult to convince the public that there is a conservation
value to restricting recreation to reduce disturbance. We studied threatened western snowy plovers (Charadrius alexandrinus nivosus) at a public beach (Sands Beach, Coal Oil Point Reserve) in Santa Barbara, California (USA) before and during a period when
a barrier directed foot traffic away from a section of upper beach where snowy plovers roost. The barrier reduced disturbance
rates by more than half. Snowy plovers increased in abundance (throughout the season) and their distribution contracted to
within the protected area. Snowy plovers that were outside the protected area in the morning moved inside as people began
using the beach. Experiments with quail eggs indicated an 8% daily risk of nest trampling outside the protected area. Before
protection, plovers did not breed at Coal Oil Point. During protection, snowy plovers bred in increasing numbers each year
and had high success at fledging young. These results demonstrate how recreational disturbance can degrade habitat for shorebirds
and that protecting quality habitat may have large benefits for wildlife and small impacts to recreation. 相似文献
114.
Jingyu Diao Rie Komura Tatsuya Sano Homer Pantua Kelly M. Storek Hiroko Inaba Haruhiko Ogawa Cameron L. Noland Yutian Peng Susan L. Gloor Donghong Yan Jing Kang Anand Kumar Katakam Michael Volny Peter Liu Nicholas N. Nickerson Wendy Sandoval Cary D. Austin Jeremy Murray Steven T. Rutherford Mike Reichelt Yiming Xu Min Xu Hayato Yanagida Junichi Nishikawa Patrick C. Reid Christian N. Cunningham Sharookh B. Kapadia 《Journal of bacteriology》2021,203(13)
115.
Erika Chavira-Suárez Alejandro Sandoval Ricardo Felix Mónica Lamas 《Biochemical and biophysical research communications》2011,404(2):678
Normal vision depends on the correct function of retinal neurons and glia and it is impaired in the course of diabetic retinopathy. Müller cells, the main glial cells of the retina, suffer morphological and functional alterations during diabetes participating in the pathological retinal dysfunction. Recently, we showed that Müller cells express the pleiotropic protein potassium channel interacting protein 3 (KChIP3), an integral component of the voltage-gated K+ channels KV4. Here, we sought to analyze the role of KChIP3 in the molecular mechanisms underlying hyperglycemia-induced phenotypic changes in the glial elements of the retina. The expression and function of KChIp3 was analyzed in vitro in rat Müller primary cultures grown under control (5.6 mM) or high glucose (25 mM) (diabetic-like) conditions. We show the up-regulation of KChIP3 expression in Müller cell cultures under high glucose conditions and demonstrate a previously unknown interaction between the KV4 channel and KChIP3 in Müller cells. We show evidence for the expression of a 4-AP-sensitive transient outward voltage-gated K+ current and an alteration in the inactivation of the macroscopic outward K+ currents expressed in high glucose-cultured Müller cells. Our data support the notion that induction of KChIP3 and functional changes of KV4 channels in Müller cells could exert a physiological role in the onset of diabetic retinopathy. 相似文献
116.
Wilson DG Phamluong K Li L Sun M Cao TC Liu PS Modrusan Z Sandoval WN Rangell L Carano RA Peterson AS Solloway MJ 《The Journal of cell biology》2011,193(5):935-951
Melanoma inhibitory activity member 3 (MIA3/TANGO1) [corrected] is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein. Recent in vitro studies have shown that it is required for the loading of collagen VII, but not collagen I, into COPII-coated transport vesicles. In this paper, we show that mice lacking Mia3 are defective for the secretion of numerous collagens, including collagens I, II, III, IV, VII, and IX, from chondrocytes, fibroblasts, endothelial cells, and mural cells. Collagen deposition by these cell types is abnormal, and extracellular matrix composition is compromised. These changes are associated with intracellular accumulation of collagen and the induction of a strong unfolded protein response, primarily within the developing skeleton. Chondrocyte maturation and bone mineralization are severely compromised in Mia3-null embryos, leading to dwarfism and neonatal lethality. Thus, Mia3's role in protein secretion is much broader than previously realized, and it may, in fact, be required for the efficient secretion of all collagen molecules in higher organisms. 相似文献
117.
Sandoval R González A Caviedes A Pancetti F Smalla KH Kaehne T Michea L Gundelfinger ED Wyneken U 《Journal of neurochemistry》2011,118(5):760-772
Nitric oxide (NO) has been proposed to down-regulate NMDA receptors (NMDA-Rs) in a homeostatic manner. However, NMDA-R-dependent NO synthesis also can cause excitotoxic cell death. Using bicuculline-stimulated hippocampal and cortical cell cultures, we have addressed the role of the brain-derived neurotrophic factor-NO pathway in NMDA-R down-regulation. This pathway protected cortical cells from NMDA-induced death and led to NMDA-R inhibition. In contrast, no evidence was gained for the presence of this protective pathway in hippocampal neurons, in which NMDA-induced NO synthesis was confirmed to be toxic. Therefore, opposing effects of NO depended on the activation of different signalling pathways. The pathophysiological relevance of this observation was investigated in synaptosomes and post-synaptic densities isolated from rat hippocampi and cerebral cortices following kainic acid-induced status epilepticus. In cortical, but not in hippocampal synaptosomes, brain-derived neurotrophic factor induced NO synthesis and inhibited NMDA-R currents present in isolated post-synaptic densities. In conclusion, we identified a NO-dependent homeostatic response in the rat cerebral cortex induced by elevated activity. A low performance of this pathway in brain areas including the hippocampus may be related to their selective vulnerability in pathologies such as temporal lobe epilepsy. 相似文献
118.
Bioplastics from microorganisms 总被引:7,自引:0,他引:7
Luengo JM García B Sandoval A Naharro G Olivera ER 《Current opinion in microbiology》2003,6(3):251-260
The term 'biomaterials' includes chemically unrelated products that are synthesised by microorganisms (or part of them) under different environmental conditions. One important family of biomaterials is bioplastics. These are polyesters that are widely distributed in nature and accumulate intracellularly in microorganisms in the form of storage granules, with physico-chemical properties resembling petrochemical plastics. These polymers are usually built from hydroxy-acyl-CoA derivatives via different metabolic pathways. Depending on their microbial origin, bioplastics differ in their monomer composition, macromolecular structure and physical properties. Most of them are biodegradable and biocompatible, which makes them extremely interesting from the biotechnological point of view. 相似文献
119.
The glucose transporter isoform GLUT4 is unique among the glucose transporter family of proteins in that, in resting cells, it is sequestered very efficiently in a storage compartment. In insulin-sensitive cells, such as fat and muscle, insulin stimulation leads to release of GLUT4 from this reservoir and its translocation to the plasma membrane. This process is crucial for the control of blood and tissue glucose levels. Investigations of the composition and structure of the GLUT4 storage compartment, together with the targeting motifs that direct GLUT4 to this compartment, have been extensive but have been controversial. Recent findings have now provided a clearer consensus of opinion on the mechanisms involved in the formation of this storage compartment. However, another controversy has now emerged, which is unresolved. This concerns the issue of whether the insulin-regulated step occurs at the level of release of GLUT4 from the storage compartment or at the level at which released vesicles fuse with the plasma membrane. 相似文献
120.
Vendela K. Lagerholm Edson Sandoval‐Castellanos Dorothee Ehrich Natalia I. Abramson Adam Nadachowski Daniela C. Kalthoff Mietje Germonpr Anders Angerbjrn John R. Stewart Love Daln 《Molecular ecology》2014,23(8):2060-2071
The Pleistocene glacial cycles resulted in significant changes in species distributions, and it has been discussed whether this caused increased rates of population divergence and speciation. One species that is likely to have evolved during the Pleistocene is the Norwegian lemming (Lemmus lemmus). However, the origin of this species, both in terms of when and from what ancestral taxon it evolved, has been difficult to ascertain. Here, we use ancient DNA recovered from lemming remains from a series of Late Pleistocene and Holocene sites to explore the species' evolutionary history. The results revealed considerable genetic differentiation between glacial and contemporary samples. Moreover, the analyses provided strong support for a divergence time prior to the Last Glacial Maximum (LGM), therefore likely ruling out a postglacial colonization of Scandinavia. Consequently, it appears that the Norwegian lemming evolved from a small population that survived the LGM in an ice‐free Scandinavian refugium. 相似文献