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121.
Arne B Gjuvsland Enikö Zörgö Jeevan KA Samy Simon Stenberg Ibrahim H Demirsoy Francisco Roque Ewa Maciaszczyk‐Dziubinska Magdalena Migocka Elisa Alonso‐Perez Martin Zackrisson Robert Wysocki Markus J Tamás Inge Jonassen Stig W Omholt Jonas Warringer 《Molecular systems biology》2016,12(12)
A major rationale for the advocacy of epigenetically mediated adaptive responses is that they facilitate faster adaptation to environmental challenges. This motivated us to develop a theoretical–experimental framework for disclosing the presence of such adaptation‐speeding mechanisms in an experimental evolution setting circumventing the need for pursuing costly mutation–accumulation experiments. To this end, we exposed clonal populations of budding yeast to a whole range of stressors. By growth phenotyping, we found that almost complete adaptation to arsenic emerged after a few mitotic cell divisions without involving any phenotypic plasticity. Causative mutations were identified by deep sequencing of the arsenic‐adapted populations and reconstructed for validation. Mutation effects on growth phenotypes, and the associated mutational target sizes were quantified and embedded in data‐driven individual‐based evolutionary population models. We found that the experimentally observed homogeneity of adaptation speed and heterogeneity of molecular solutions could only be accounted for if the mutation rate had been near estimates of the basal mutation rate. The ultrafast adaptation could be fully explained by extensive positive pleiotropy such that all beneficial mutations dramatically enhanced multiple fitness components in concert. As our approach can be exploited across a range of model organisms exposed to a variety of environmental challenges, it may be used for determining the importance of epigenetic adaptation‐speeding mechanisms in general. 相似文献
122.
Abdallah M. Samy Badereddin B. Annajar Mostafa Ramadhan Dokhan Samia Boussaa A. Townsend Peterson 《PLoS neglected tropical diseases》2016,10(2)
Cutaneous leishmaniasis ranks among the tropical diseases least known and most neglected in Libya. World Health Organization reports recognized associations of Phlebotomus papatasi, Psammomys obesus, and Meriones spp., with transmission of zoonotic cutaneous leishmaniasis (ZCL; caused by Leishmania major) across Libya. Here, we map risk of ZCL infection based on occurrence records of L. major, P. papatasi, and four potential animal reservoirs (Meriones libycus, Meriones shawi, Psammomys obesus, and Gerbillus gerbillus). Ecological niche models identified limited risk areas for ZCL across the northern coast of the country; most species associated with ZCL transmission were confined to this same region, but some had ranges extending to central Libya. All ENM predictions were significant based on partial ROC tests. As a further evaluation of L. major ENM predictions, we compared predictions with 98 additional independent records provided by the Libyan National Centre for Disease Control (NCDC); all of these records fell inside the belt predicted as suitable for ZCL. We tested ecological niche similarity among vector, parasite, and reservoir species and could not reject any null hypotheses of niche similarity. Finally, we tested among possible combinations of vector and reservoir that could predict all recent human ZCL cases reported by NCDC; only three combinations could anticipate the distribution of human cases across the country. 相似文献
123.
Cristian A. Acevedo Rodrigo A. Somoza Caroline Weinstein-Oppenheimer Samy Silva Macarena Moreno Elizabeth Sánchez Fernando Albornoz Manuel E. Young William MacNaughtan Javier Enrione 《Bioprocess and biosystems engineering》2013,36(3):317-324
Gelatin-/chitosan-/hyaluronan-based biomaterials are used in tissue engineering as cell scaffolds. Three gamma radiation doses (1, 10 and 25 kGy) were applied to scaffolds for sterilization. Microstructural changes of the irradiated polymers were evaluated by using scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). A dose of 25 kGy produced a rough microstructure with a reduction of the porosity (from 99 to 96 %) and pore size (from 160 to 123 μm). Radiation also modified the glass transition temperature between 31.2 and 42.1 °C (1 and 25 kGy respectively). Human skin cells cultivated on scaffolds irradiated with 10 and 25 kGy proliferated at 48 h and secreted transforming growth factor β3 (TGF-β3). Doses of 0 kGy (non-irradiated) or 1 kGy did not stimulate TGF-β3 secretion or cell proliferation. The specific growth rate and lactate production increased proportionally to radiation dose. The use of an appropriate radiation dose improves the cell scaffold properties of biomaterials. 相似文献
124.
Of 20 common amino acids added individually to ground beef patties prior to frying at 191°C for 4 min, only proline enhanced the formation of mutagenic activity. Proline cognates, except for esters, were inactive as was hydroxyproline. Mutagenic activity from untreated and proline-treated fried ground beef exhibited similar chromatographic behavior. 相似文献
125.
Showket Ahmad Dar Muneer Ahmad Sofi Mohamed El-Sharnouby Mudasir Hassan Rizwan Rashid Sajad Hussain Mir Yahya Al Naggar Mohamed Salah Ivana Tlak Gajger Samy Sayed 《Saudi Journal of Biological Sciences》2021,28(8):4147-4154
The current studies were carried out in the three experimental locations of Kashmir valley during 2013 to 2016. The species Andrena cineraria formed the dense nest aggregations in plan grounds, barren lands and hilly areas near the fruit orchards and other landscapes with clay loam soil type. The species start flying and foraging in the orchards from April till July. The nests were allodalous, 29–36 cm in depth, with cells located obliquely around the main barrow. The nests were dense with a maximum density of 11.09 nests/m2 observed in landscapes of Budgam. The barrow diameters were found varying with depth from main entrance. The maximum barrow diameter recorded was 2.05 mm. At certain depth, the female constructs the first cell and the upper nest burrow is vertical and lower is oblique. The nest entrance is generally hidden under the tumulus. In the depth of average 30.48 cm, each cell directly opens to main burrow either alternately or unilaterally. The cell number, diameter, and length varied with depth. Foraging behaviour of A. cineraria on various fruit crops and other shrubs and social forestry trees were determined and the abundance, visitation rate, total visits and time spend per flower were found significant, especially on fruit crops. The significance of the studies is important for the melittologists, as it will help in the conservation of bee fauna. The study is also important in using this species for pollination purpose and would also help to detect and understand the possible pre-adaptation of species in temperate region of Kashmir valley. 相似文献
126.
Samy L. Habib 《Cell cycle (Georgetown, Tex.)》2014,13(6):869-870
127.
128.
Bionomics of phlebotomine sand flies (Diptera: Psychodidae) in the province of Al-Baha, Saudi Arabia
The bionomics of phlebotomine sand flies (Diptera: Psychodidae) were studied for two successive years (January 1996-December 1997) at 12 collecting stations representing six sectors of the province of Al-Baha, Saudi Arabia. The predominant species was Phlebotomus bergeroti (41.7%), followed by lesser numbers of Phlebotomus sergenti (11%), Phlebotomus arabicus (10.6%), Sergentomyia tiberiadis (10.5%), Phlebotomus papatasi (10.2%), Sergentomyia antennata (9.6%), Phlebotomus alexandri (3%), Phlebotomus orientalis (2.3%) and Sergentomyia clydei (1.1%). The distribution of the collected species including species that are elsewhere known to act as vectors of human cutaneous leishmaniasis were distributed across different altitudes in Al-Baha. P. bergeroti, P. papatasi and P. arabicus were more abundant indoors; however, P. sergenti was more abundant outdoors. Sand fly populations exhibited three patterns of seasonal abundance in terms of their monthly activity. P. bergeroti, P. sergenti and P. arabicus were found to be naturally infected with Leishmania-like flagellates at an infection rate of 0.2%. 相似文献
129.
Tim Newbold Tom Reader Ahmed El‐Gabbas Wiebke Berg Wael M. Shohdi Samy Zalat Sherif Baha El Din Francis Gilbert 《Oikos》2010,119(8):1326-1334
Species distribution models are a very popular tool in ecology and biogeography and have great potential to help direct conservation efforts. Models are traditionally tested by using half the original species records to build the model and half to evaluate it. However, this can lead to overly optimistic estimates of model accuracy, particularly when there are systematic biases in the data. It is better to evaluate models using independent data. This study used independent species records from a new to survey to provide a more rigorous evaluation of distribution‐model accuracy. Distribution models were built for reptile, amphibian, butterfly and mammal species. The accuracy of these models was evaluated using the traditional approach of partitioning the original species records into model‐building and model‐evaluating datasets, and using independent records collected during a new field survey of 21 previously unvisited sites in diverse habitat types. We tested whether variation in distribution‐model accuracy among species could be explained by species detectability, range size, number of records used to build the models, and body size. Estimates of accuracy derived using the new species records correlated positively with estimates generated using the traditional data‐partitioning approach, but were on average 22% lower. Model accuracy was negatively related to range size and number of records used to build the models, and positively related to the body size of butterflies. There was no clear relationship between species detectability and model accuracy. The field data generally validated the species distribution models. However, there was considerable variation in model accuracy among species, some of which could be explained by the characteristics of species. 相似文献
130.
Jia Lu Xue Samy Cecioni Li He Sbastien Vidal Jean-Pierre Praly 《Carbohydrate research》2009,344(13):1646-1653
Glycosidation of sugar peracetates (d-gluco, d-galacto) with SnCl4 and CF3CO2Ag led to either 1,2-cis-, or 1,2-trans-glycosides, depending primarily on the alcohols used. In particular, 1,2-trans-glycosides, expected from acyl-protected glycosyl donors, were formed in high yields with alcohols sharing specific features such as bulkiness, presence of electron-withdrawing groups or polyethoxy motifs. In contrast, simple alcohols afforded 1:1 mixtures of 2,3,4,6-tetra-O-acetyl, and 3,4,6-tri-O-acetyl 1,2-cis-glycosides due to anomerization and/or acid-catalyzed fragmentation of 1,2-orthoester intermediates. After reacetylation or deacetylation, acetylated or fully deprotected 1,2-cis-glycosides (α-d-gluco, α-d-galacto) were obtained in 90% yields by a simple and direct method. 相似文献