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921.
Iwona Kuszczak Rajneet Kuner Sue E. Samson Ashok K. Grover 《Molecular and cellular biochemistry》2010,339(1-2):293-300
Pig coronary artery smooth muscle expresses the Na+–Ca2+-exchanger NCX1 and the sarco/endoplasmic reticulum (SER) Ca2+ pump SERCA2. NCX has been proposed to play a role in refilling the SER Ca2+ pool. Caveolae may also direct Ca2+ traffic during cell signaling. Here, we use immunofluorescence microscopy to determine if there is proximity between NCX1, SERCA2, and the caveolar protein caveolin-1. Stacks of images of cell surface domains were analyzed. Image stacks for one protein were analyzed for overlap with another protein, with and without randomization or image shifting. Within the resolution of light microscopy, there is significant overlap in the distributions of NCX1, SERCA2, and caveolin-1 but the three proteins are not always co-localized. The proximity between NCX1, SERCA2 is consistent with the assertion that NCX may supply Ca2+ for refilling the SER but this relationship is only partial. Similarly, caveolae may direct traffic in some Ca2+ signaling pathways but not others. 相似文献
922.
Yuzuru Mikami Katsukiyo Yazawa Kazutaka Fukushima Tadashi Arai Shun-ichi Udagawa Robert A. Samson 《Mycopathologia》1989,108(3):195-199
The production of paecilotoxin from various isolates of Paecilomyces lilacinus was studied using three different media and high performance liquid chromatography (HPLC). Alkaline medium was found to be suitable for the production of the toxins. Among 20 strains tested, 19 including four clinical isolates were found to produce the toxins. Production patterns of paecilotoxins were very similar in each strain and the main toxins were A and B. 相似文献
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925.
Sood A Abid M Sauer C Hailemichael S Foster M Török B Török M 《Bioorganic & medicinal chemistry letters》2011,21(7):2044-2047
A potential therapeutic approach for Alzheimer’s disease is to reduce the amount of toxic amyloid-beta oligomers and fibrillar amyloid plaques. In order to contribute to this approach the ability of small organofluorine compounds that were previously reported as successful inhibitors of fibrillogenesis to destabilize preformed fibrils of the amyloid-beta peptide was studied. These organofluorine molecules including chiral compounds were tested in vitro using standard methods based on Thioflavin-T (THT) fluorescence spectroscopy, atomic force microscopy (AFM) and Fourier-transform infrared spectroscopy (FTIR). It was observed that 5′-halogen substituted 3,3,3-trifluoromethyl-2-hydroxyl-(indol-3-yl)-propionic acid esters showed significant activity in the disassembly of the preformed fibrils. Since the same compounds were identified as strong fibrillogenesis inhibitors as well, this dual action makes them promising candidates for further drug development. 相似文献
926.
The zebrafish sensory lateral line system has emerged as a powerful model for the mechanistic study of collective cell migration and morphogenesis. Recent work has uncovered the details of a signaling network involving the Wnt/β-catenin, Fgf and Delta-Notch pathways that patterns the migrating lateral line primordium into distinct regions. Cells within these regions exhibit different fundamental behaviors that together orchestrate normal lateral line morphogenesis. In this review, we summarize the signaling network that patterns the migrating lateral line primordium and describe how this patterning coordinates crucial morphogenic cell behaviors.Key words: lateral line, Wnt signaling, Fgf signaling, collective migration, morphogenesis 相似文献
927.
Biotechnology Letters - Diversity in backbone polymer composition makes hydrogel-based resources open to broad spectrum of applications. Biomacromolecules which have reactive functional groups in... 相似文献
928.
Yu Wang M. Aman Haqmal Yue-dong Liang Inam Muhammad Xiao-Ou Zhao Emad Mohammed Elken Yun-Hang Gao Yu Jia Cheng-guang He Yi-Ming Wang Ling-Cong Kong Hong-Xia Ma 《Microbial biotechnology》2022,15(9):2337-2350
Pseudomonas sp. strain 166 was isolated from soil samples from Changbai Mountains. A novel bacteriocin PA166 from Pseudomonas sp. 166 was purified using ammonium sulfate, dextran gel chromatography column and Q-Sepharose column chromatography successively. The molecular mass of bacteriocin PA166 was found to be 49.38 kDa by SDS-PAGE and liquid chromatography–mass spectrometry (MS)/MS. Bacteriocin PA166 showed stability at a wide range of pH (2–10), and thermal stability (40, 60, 80 and 100°C). The bacteriocin PA166 antimicrobial activity was slightly inhibited by Ca2+, K+ and Mg2+. The minimum bactericidal concentrations of bacteriocin PA166 against five Pasteurella multocida strains ranged from 2 to 8 μg ml−1. Bacteriocin PA166 showed low cytotoxicity and a higher treatment index (TI = 82.51). Fluorescence spectroscopy indicated that bacteriocin PA166 destroyed the cell membrane to exert antimicrobial activity. In summary, bacteriocin PA166 had strong antibacterial activity, high TI and low toxicity, and hence could serve as a potential clinical therapeutic drug. 相似文献
929.
Esther M. Ellis John C. Neatherlin Mark Delorey Melvin Ochieng Abdinoor Haji Mohamed Daniel Ondari Mogeni Elizabeth Hunsperger Shem Patta Stella Gikunju Lilian Waiboic Barry Fields Victor Ofula Samson Limbaso Konongoi Brenda Torres-Velasquez Nina Marano Rosemary Sang Harold S. Margolis Joel M. Montgomery Kay M. Tomashek 《PLoS neglected tropical diseases》2015,9(4)
Dengue appears to be endemic in Africa with a number of reported outbreaks. In February 2013, several individuals with dengue-like illnesses and negative malaria blood smears were identified in Mombasa, Kenya. Dengue was laboratory confirmed and an investigation was conducted to estimate the magnitude of local transmission including a serologic survey to determine incident dengue virus (DENV) infections. Consenting household members provided serum and were questioned regarding exposures and medical history. RT-PCR was used to identify current DENV infections and IgM anti-DENV ELISA to identify recent infections. Of 1,500 participants from 701 households, 210 (13%) had evidence of current or recent DENV infection. Among those infected, 93 (44%) reported fever in the past month. Most (68, 73%) febrile infected participants were seen by a clinician and all but one of 32 participants who reportedly received a diagnosis were clinically diagnosed as having malaria. Having open windows at night (OR = 2.3; CI: 1.1–4.8), not using daily mosquito repellent (OR = 1.6; CI: 1.0–2.8), and recent travel outside of Kenya (OR = 2.5; CI: 1.1–5.4) were associated with increased risk of DENV infection. This survey provided a robust measure of incident DENV infections in a setting where cases were often unrecognized and misdiagnosed. 相似文献
930.
Charlotte Rosso Christine Pires Jean-Christophe Corvol Flore Baronnet Sophie Crozier Anne Leger Sandrine Deltour Romain Valabregue Mélika Amor-Sahli Stéphane Lehéricy Didier Dormont Yves Samson 《PloS one》2015,10(3)