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51.
Sams Gary R. Cocchiaro Gerald F. Bell William J. 《In vitro cellular & developmental biology. Plant》1978,14(11):956-960
Summary The time course of juvenile hormone (JH) metabolism is examined in cultures ofPeriplaneta americana fat body and ovaries in medium containingManduca sexta carrier protein or cockroach hemolymph. In the absence ofM. sexta carrier protein or cockroach hemolymph, both tissues extensively catabolize exogenous [3H]JH in the medium. Addition of the carrier protein or hemolymph to the culture system prevents the hydrolysis of the hormone
in the medium. Within the tissues JH is degraded whether or not carrier protein or hemolymph is present which suggests that
the protective role of these molecules is exclusively extracellular. Incubation of [3H]JH with medium preconditioned with tissue results in destruction of the hormone. This suggests that the fat body secretes
esterases into the medium. In contrast, the ovarioles hydrolyze the hormone by means of cell-associated enzyme. The relationship
of these phenomena to insect development is discussed.
This work supported by NSF Grant PCM 76-02229 and University of Kansas Biomedical Sciences Grant RR-07037. 相似文献
52.
53.
T. R. Warne L. G. Hickok C. E. Sams & D. L. Vogelien 《Plant, cell & environment》1999,22(8):1027-1034
The roles of Na+ and K+ (Rb+) uptake were further studied in a NaCl-tolerant strain of Ceratopteris richardii containing the stl2 mutation by direct comparison with the wild-type strain. In addition to Na+ tolerance, stl2 also confers tolerance to Mg2+ and sensitivity to K+. In addition to higher K+ (Rb+) uptake at concentrations commonly associated with low-affinity K+ transport, stl2 maintained higher uptake down to 0·1 mol m–3 Rb+. Up to a 25-fold excess of Na+ had little effect in either genotype on K+ (Rb+) uptake at low concentrations, i.e. 0·2 and 0·5 mol m–3 RbCl. Pretreatment with K+ (20 mol m–3) inhibited uptake of K+ (Rb+) in the wild type, whereas concurrent inclusion of K+ inhibited uptake of Rb+ more in stl2. In the absence of K+, Na+ uptake (0·01–60 mol m–3) was nearly identical in the wild type and stl2. K+ inhibited Na+ uptake more effectively in stl2 than the wild type, especially at 60 mol m–3 Na+. Greater inhibition of K+ uptake in stl2 occurred with MgCl2 or TEA (tetraethylammonium chloride) preincubation or with simultaneous inclusion of Al3+ (Al2SO4). The higher effective velocity of K+ uptake at a wide range of concentrations and the enhanced selectivity for K+ and against Na+ contribute to the preservation of higher cytosolic K+ and lower Na+ under salinity stress. 相似文献
54.
BACKGROUND: The NASA/American Cancer Society (ACS) flow cytometer can simultaneously analyze the electronic nuclear volume (ENV) and DNA content of cells. This study describes the schematics, resolution, reproducibility, and sensitivity of biological standards analyzed on this unit. METHODS: Calibrated beads and biological standards (lymphocytes, trout erythrocytes [TRBC], calf thymocytes, and tumor cells) were analyzed for ENV versus DNA content. Parallel data (forward scatter versus DNA) from a conventional flow cytometer were obtained. RESULTS: ENV linearity studies yielded an R value of 0.999. TRBC had a coefficient of variation (CV) of 1.18 +/- 0.13. DNA indexes as low as 1.02 were detectable. DNA content of lymphocytes from 42 females was 1.9% greater than that for 60 males, with a noninstrumental variability in total DNA content of 0.5%. The ENV/DNA ratio was constant in 15 normal human tissue samples, but differed in the four animal species tested. The ENV/DNA ratio for a hypodiploid breast carcinoma was 2.3 times greater than that for normal breast tissue. CONCLUSIONS: The high-resolution ENV versus DNA analyses are highly reliable, sensitive, and can be used for the detection of near-diploid tumor cells that are difficult to identify with conventional cytometers. ENV/DNA ratio may be a useful parameter for detection of aneuploid populations. 相似文献
55.
G H Sams B M Hargis P S Hargis 《Comparative biochemistry and physiology. B, Comparative biochemistry》1990,96(3):585-590
1. Fatty acid binding protein (A-FABP) was isolated from chicken adipose cytosol. 2. Relative mol. wt of chicken A-FABP was determined to be 14,400 from SDS-polyacrylamide electrophoresis; the pI was 5.1; and amino acid composition data indicated structural homology with mammalian heart and adipose FABPs. 3. Polyclonal antisera prepared against A-FABP exhibited monospecificity for chicken A-FABP and no cross-reactivity with chicken liver proteins was observed. 4. Determination of relative ligand binding characteristics indicated A-FABP exhibited greatest binding activity in response to linoleate, followed by oleate, palmityl CoA and palmitate; no binding affinity for cholesterol was detected. 相似文献
56.
Celiac disease is a common small intestinal inflammatory condition induced by wheat gluten and related proteins from rye and barley. Left untreated, the clinical presentation of CD can include failure to thrive, malnutrition, and distension in juveniles. The disease can additionally lead to vitamin deficiencies, anemia, and osteoporosis. Therefore, CD potentially negatively affected fitness in past populations utilizing wheat, barley, and rye. Previous analyses of CD risk variants have uncovered evidence for positive selection on some of these loci. These studies also suggest the possibility that risk for common autoimmune conditions such as CD may be the result of positive selection on immune related loci in the genome to fight infection. Under this evolutionary scenario, disease phenotypes may be a trade-off from positive selection on immunity. If this hypothesis is generally true, we can expect to find a signal of natural selection when we survey across the network of loci known to influence CD risk. This study examines the non-HLA autosomal network of gene loci associated with CD risk in Europe. We reject the null hypothesis of neutrality on this network of CD risk loci. Additionally, we can localize evidence of selection in time and space by adding information from the genome of the Tyrolean Iceman. While we can show significant differentiation between continental regions across the CD network, the pattern of evidence is not consistent with primarily recent (Holocene) selection across this network in Europe. Further localization of ancient selection on this network may illuminate the ecological pressures acting on the immune system during this critically interesting phase of our evolution. 相似文献
57.
Mehta SK Crucian B Pierson DL Sams C Stowe RP 《Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology》2007,14(1):P21-P25
Numerous studies have indicated that dysregulation of the immune system occurs during or after spaceflight. Using 21 day 6 head-down tilt bed rest as a spaceflight analog, this study describes the effects of a daily artificial gravity (AG) countermeasure treatment on immunity, stress, and reactivation of clinically important latent herpes viruses. Blood, saliva, and urine samples were collected from each of the 15 male test subjects (8 treatment, 7 control) periodically throughout the study. The immune assessment consisted of a comprehensive peripheral immunophenotype analysis, intracellular cytokine profiles, and measurement of T cell function. With the exception of mild reactivation of Epstein-Barr (EBV) and Varicella zoster (VZV) viruses, no significant changes in immune function were observed, suggesting that the AG countermeasure and the 21 day head-down tilt bed rest regimen had no adverse effect on immune function. 相似文献
58.
Ling A Plewe M Gonzalez J Madsen P Sams CK Lau J Gregor V Murphy D Teston K Kuki A Shi S Truesdale L Kiel D May J Lakis J Anderes K Iatsimirskaia E Sidelmann UG Knudsen LB Brand CL Polinsky A 《Bioorganic & medicinal chemistry letters》2002,12(4):663-666
A series of alkylidene hydrazide derivatives containing an alkoxyaryl moiety was optimized. The resulting hydrazide-ethers were competitive antagonists at the human glucagon receptor. Pharmacokinetic experiments showed fast clearance of most of the compounds tested. A representative compound [4-hydroxy-3-cyanobenzoic acid (4-isopropylbenzyloxy-3,5-dimethoxymethylene)hydrazide] with an IC50 value of 20 nM was shown to reduce blood glucose levels in fasted rats. 相似文献
59.
Background
Binding of serum components by surface M-related proteins, encoded by the emm genes, in streptococci constitutes a major virulence factor in this important group of organisms. The present study demonstrates fibrinogen binding by S. iniae, a Lancefield non-typeable pathogen causing devastating fish losses in the aquaculture industry and an opportunistic pathogen of humans, and identifies the proteins involved and their encoding genes. 相似文献60.
D L Harm R T Jennings J V Meck M R Powell L Putcha C P Sams S M Schneider L C Shackelford S M Smith P A Whitson 《Journal of applied physiology》2001,91(5):2374-2383
This minireview provides an overview of known and potential gender differences in physiological responses to spaceflight. The paper covers cardiovascular and exercise physiology, barophysiology and decompression sickness, renal stone risk, immunology, neurovestibular and sensorimotor function, nutrition, pharmacotherapeutics, and reproduction. Potential health and functional impacts associated with the various physiological changes during spaceflight are discussed, and areas needing additional research are highlighted. Historically, studies of physiological responses to microgravity have not been aimed at examining gender-specific differences in the astronaut population. Insufficient data exist in most of the discipline areas at this time to draw valid conclusions about gender-specific differences in astronauts, in part due to the small ratio of women to men. The only astronaut health issue for which a large enough data set exists to allow valid conclusions to be drawn about gender differences is orthostatic intolerance following shuttle missions, in which women have a significantly higher incidence of presyncope during stand tests than do men. The most common observation across disciplines is that individual differences in physiological responses within genders are usually as large as, or larger than, differences between genders. Individual characteristics usually outweigh gender differences per se. 相似文献