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841.
Tetrapeptide substrates for the discrimination among kallikreins and other trypsin-like serine proteinases 总被引:1,自引:0,他引:1
E S Prado M S Araújo-Viel M A Juliano L Juliano R C Stella C A Sampaio 《Biological chemistry Hoppe-Seyler》1986,367(3):199-205
The three tetrapeptides Ac-Phe-Arg-Arg-Val-NH2 (I), Ac-Phe-Arg-Arg-Pro-NH2 (II) and Ac-Phe-Lys-Arg-Val-NH2 (III) were shown to form a most convenient substrate system for the discrimination of the serine proteinases listed below. Tissue kallikreins (porcine pancreatic, horse and human urinary) have the unique feature of cleaving well the Arg-Arg bond in peptide I (P'2 = Val), hardly splitting it in peptide II (P'2 = Pro). The kcat/Km for the hydrolysis of peptide II by horse urinary kallikrein was 600-fold lower than that for peptide I. Trypsin, plasma kallikreins (human and rat), tonin and rat urinary kallikrein were distinguished from each other by the sequence of the N-terminal fragments formed in the hydrolysis of peptides I and/or II. Differences in the cleavage sites in these peptides are explained by differences in the specificities of the proteinase subsite S2 and/or in their preference for Arg or Lys residues. The three tetrapeptides were not substrates for plasmin. 相似文献
842.
Manoel F. Cardoso Carlos A. Nobre David M. Lapola Marcos D. Oyama Gilvan Sampaio 《Global Ecology and Biogeography》2008,17(2):222-235
Aim This study aims to improve the formulation and results of the Brazilian Center for Weather Forecasting and Climate Studies Potential Vegetation Model (CPTEC-PVM) by developing a new parameterization for the long-term occurrence of fire in regions of potential savannas in the tropics. Compared with the relatively slow processes of carbon uptake and growth in vegetation, fast mortality and biomass consumption by fires may favour grasses and reduce tree coverage.
Location The tropics.
Methods For finding large-scale relationships between fires and other environmental factors, we made two main simplifying assumptions. First, lightning is the most important source of ignition for natural fires. Second, over continental areas in the tropics, lightning is mainly related to the zonal flux of moisture transport.
Results The parameterization of fire occurrence was built based on a simple empirical relationship, combining information on mean and intra-annual variance of the zonal wind.
Main conclusions The implementation of this new relationship improved the formulation and the results of the CPTEC-PVM. As a result of this new parameter, the accuracy of the model in allocating the correct vegetation (seasonal forests) instead of savannas for large regions in India and Southeast Asia is now substantially higher than in previous studies. 相似文献
Location The tropics.
Methods For finding large-scale relationships between fires and other environmental factors, we made two main simplifying assumptions. First, lightning is the most important source of ignition for natural fires. Second, over continental areas in the tropics, lightning is mainly related to the zonal flux of moisture transport.
Results The parameterization of fire occurrence was built based on a simple empirical relationship, combining information on mean and intra-annual variance of the zonal wind.
Main conclusions The implementation of this new relationship improved the formulation and the results of the CPTEC-PVM. As a result of this new parameter, the accuracy of the model in allocating the correct vegetation (seasonal forests) instead of savannas for large regions in India and Southeast Asia is now substantially higher than in previous studies. 相似文献
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