Morphofunctional characteristics of enzyme activity of the muscles in ontogenesis and after physical load

By means of histochemical methods using cytospectrophotometer in femoral muscles of white rats 1-, 3- and 12-month-old enzymatic activity of anaerobic and aerobic cycles has been estimated. The greatest changes occur after 20 days of physical load in 1- and 12-month-old animals. The semitendinous muscle (ventral origin), consisting mainly of red muscle fibers, works chiefly in aerobic regime and possesses a more manifested succinate dehydrogenase activity, and the quadriceps muscle (dorsal origin) consists principally of white fibers and its lactate dehydrogenase activity changes more noticeably.     

Analysis of substances released from Zajdela ascites hepatoma cells exposed to UV radiation of different wavelengths. III. Release of nucleoproteins and carbohydrates]

Irradiation of the Zaidela ascite hepatoma cells with physiological doses of shortwave length (254 nm) and longwave length (300-380 nm) UV light (far and near UV radiation) is accompanied by the release of ribonucleoproteins (RNP) from the cells, whose amounts increase with dose. Irradiation with far and near UV light leads to the release of high-molecular and low-molecular RNP, respectively. No deoxyribonucleoprotein were found among the released substances. Non-protein fractions, released from irradiated cells, contain carbohydrate-like substances. At maximum far and near UV doses the amounts of these substances constitute 180-190% of the control and 6% of their amount in intact cells. After irradiation with far UV light, relatively high-molecular carbohydrates are released, while near UV light treatment induces the release of low-molecular carbohydrates. The criteria tested show that the efficiency of far UV light exceeds that of near UV light by one order.     

Regularity of changes in magnetic characteristics of Saccharomyces cerevisiae yeast cells at various stages of culture growth

Correlation between cell cycle of the synchronous yeast culture and ESR signal intensity at g 2.2 and 77 K was studied. It was shown that the maximal intensity of ESR signal was reached 10-15 min before the beginning of intensive cell division. The ESR signal with g 2.2 (77 K) is caused by the spin-glass like structure. The "freezing" temperature of these spin-glasses was measured.     

Photoreactivation of cells and phages injuried by ultraviolet radiation in the ecological long-wave band]

Photoreactivation (PR) was measured after inactivation by far (254 nm), middle (300-315 nm) and near (315-400 nm) UV radiation of Paramecium caudatum and 8 strains of Escherichia coli differing in PR and dark repair capability. PR volume was high and practically the same after irradiation by far and middle UV, but PR was not observed in near UV-inactivated cells of all the strains. It is proposed that pyrimidine dimers are not significant in near UV lethal lesions in cells, as near UV-irradiated phages (T7 and lambdacI 857) are not photoreactivated in undamaged host bacterial cells.     

Astroglial Mechanisms of Ketamine Action Include Reduced Mobility of Kir4.1-Carrying Vesicles

Neurochemical Research - The finding that ketamine, an anaesthetic, can elicit a rapid antidepressant effect at low doses that lasts for weeks in patients with depression is arguably a major...