Ultrastructure of the cerebral cortex of the cat in the early recovery period after short-term anoxia

The ultrastructure of cat sensomotor cortex has been studied during a 15-minute recovery after 2.5-6-minute oxygen supply cessation. An increase of osmopholia of free and endoplasmic reticulum bound ribosomes was detected in addition to a great number of altered mitochondria with longitudinal crystal arrangement. Besides, numerous activated synapses, local destructive changes of membrane complexes in dendrite and myelinated axons cytoplasm and glycogen granule accumulation in neuroglia were noticed. During the recovery period more prominent changes were shown after a 6-minute anoxia than after a 2.5-minute one.     

Degradation of alkylsulfates by a Pseudomonas aeruginosa culture immobilized on a polyvinyl alcohol fiber

Pseudomonas aeruginosa cells capable of destroying alkyl sulfates, anionic surfactants, were immobilised on activated polyvinyl alcohol fibres. The immobilised cells could decompose SDS. When the immobilised cells were used repeatedly, their biomass increased but the activity hardly changed.     

Semisyngeneic transplantation of hematopoietic cells of native and cultured embryonic liver

The protective ability and graft-versus-host (GVH) activity in parental strain hematopoietic fetal liver cells (FLC) transplanted to irradiated F1 hybrids were evaluated quantitatively. A 21-day survival of more than 80% of semi-syngeneic mouse recipients required the injection of 2-5 X 10(6) nucleated fetal liver cells (FLC). The same effect could be obtained with FLC cultivated for 4-15 days. 5 to 25 X 10(6) parental FLC were necessary to induce a considerable GVH mortality within 2-3 months after transplantation. Thus, the minimal cell doses of both native and cultivated FLC enough for the maximal protective effect have proved ineffective for the provocation of the fetal GVH disease. Hematopoietic cells from long-term FLC cultures had a low protective potential though they could contain high CFUs concentration. This discrepancy shows clearly that such polypotent precursors as CFUs have no ability to restore hematopoiesis, in other words they cannot be totipotent stem cells.