全文获取类型
收费全文 | 478篇 |
免费 | 17篇 |
国内免费 | 2篇 |
专业分类
497篇 |
出版年
2023年 | 6篇 |
2022年 | 16篇 |
2021年 | 37篇 |
2020年 | 21篇 |
2019年 | 33篇 |
2018年 | 23篇 |
2017年 | 15篇 |
2016年 | 20篇 |
2015年 | 11篇 |
2014年 | 38篇 |
2013年 | 40篇 |
2012年 | 35篇 |
2011年 | 50篇 |
2010年 | 28篇 |
2009年 | 14篇 |
2008年 | 19篇 |
2007年 | 21篇 |
2006年 | 17篇 |
2005年 | 11篇 |
2004年 | 8篇 |
2003年 | 8篇 |
2002年 | 9篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 4篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1972年 | 1篇 |
排序方式: 共有497条查询结果,搜索用时 15 毫秒
371.
372.
373.
374.
To realize the therapeutic potential of RNA drugs, efficient, tissue-specific and nonimmunogenic delivery technologies must be developed. Here we show that exosomes-endogenous nano-vesicles that transport RNAs and proteins-can deliver short interfering (si)RNA to the brain in mice. To reduce immunogenicity, we used self-derived dendritic cells for exosome production. Targeting was achieved by engineering the dendritic cells to express Lamp2b, an exosomal membrane protein, fused to the neuron-specific RVG peptide. Purified exosomes were loaded with exogenous siRNA by electroporation. Intravenously injected RVG-targeted exosomes delivered GAPDH siRNA specifically to neurons, microglia, oligodendrocytes in the brain, resulting in a specific gene knockdown. Pre-exposure to RVG exosomes did not attenuate knockdown, and non-specific uptake in other tissues was not observed. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated by the strong mRNA (60%) and protein (62%) knockdown of BACE1, a therapeutic target in Alzheimer's disease, in wild-type mice. 相似文献
375.
Essential Oils as Biocides for the Control of Fungal Infections and Devastating Pest (Tuta absoluta) of Tomato (Lycopersicon esculentum Mill.) 下载免费PDF全文
Samira Bouayad Alam Mohammed El Amine Dib Nassim Djabou Boufeldja Tabti Nassira Gaouar Benyelles Jean Costa Alain Muselli 《化学与生物多样性》2017,14(7)
Thymus capitatus and Tetraclinis articulata essential oils as well their major components (carvacrol and α‐pinene) were evaluated for their antifungal and insecticidal activities. Both oils showed good in vitro antifungal activity against Fusarium oxysporum, Aspergillus niger, Penicillium sp., Alternaria alternata, and Botrytis cinerea, the fungi causing tomato rot. In vivo results indicate the efficacies of both essential oils and carvacrol of reduce postharvest fungal pathogens, such as B. cinerea and Al. alternata that are responsible of black and gray rot of tomato fruit. Disease incidence of Al. alternata and B. cinerea decreased on average from 55% to 80% with essential oil of Th. capitatus and pure carcvacrol, while Te. articulata essential oil exhibited inhibition of fungal growth of 55% and 25% against Al. alternata and B. cinerea, respectively, with concentration of 0.4 μl/l air. The insecticidal activity of Th. capitatus and Te. articulata essential oils exhibited also a good insecticidal activity. At the concentration of 0.2 μl/ml air, the oils caused mortality over 80% for all larval stages of Tuta absoluta and 100% mortality for the first‐instar after 1.5 h only of exposure. α‐Pinene presented lower insecticidal and antifungal activities compared to essential oils of Th. capitatus, Te. articulata and pure carvacrol. Thus, these essential oils can be used as a potential source to develop control agents to manage some of the main pests and fungal diseases of tomato crops. 相似文献
376.
Eydian Zahra Mohammad Ghasemi Alaleh Ansari Samira Kamali Ali Naghi Khosravi Maryam Momtaz Sima Riki Sanaz Rafighdoost Laleh Entezari Heravi Reza 《Molecular biology reports》2022,49(5):3539-3548
Molecular Biology Reports - Mesenchymal stem cells (MSCs) from human adipose tissue and bone marrow have a great potential for use in cell therapy due to their ease of isolation, expansion, and... 相似文献
377.
Molecular and Cellular Biochemistry - MicroRNAs (miRNAs) are important molecules which implicated in various processes, such as differentiation, development, cell survival, cell apoptosis and also... 相似文献
378.
Amos Samira Mengistu Sileshi Kleinschroth Fritz 《Human ecology: an interdisciplinary journal》2021,49(5):525-537
Human Ecology - River deltas provide important livelihoods to local populations, but at the same time are under increasing anthropogenic pressure. The opening of the Gibe III dam on the Omo River... 相似文献
379.
380.