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101.
102.
Lithium Fluoride Based Electron Contacts for High Efficiency n‐Type Crystalline Silicon Solar Cells
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![点击此处可从《Liver Transplantation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
James Bullock Peiting Zheng Quentin Jeangros Mahmut Tosun Mark Hettick Carolin M. Sutter‐Fella Yimao Wan Thomas Allen Di Yan Daniel Macdonald Stefaan De Wolf Aïcha Hessler‐Wyser Andres Cuevas Ali Javey 《Liver Transplantation》2016,6(14)
Low‐resistance contact to lightly doped n‐type crystalline silicon (c‐Si) has long been recognized as technologically challenging due to the pervasive Fermi‐level pinning effect. This has hindered the development of certain devices such as n‐type c‐Si solar cells made with partial rear contacts (PRC) directly to the lowly doped c‐Si wafer. Here, a simple and robust process is demonstrated for achieving mΩ cm2 scale contact resistivities on lightly doped n‐type c‐Si via a lithium fluoride/aluminum contact. The realization of this low‐resistance contact enables the fabrication of a first‐of‐its‐kind high‐efficiency n‐type PRC solar cell. The electron contact of this cell is made to less than 1% of the rear surface area, reducing the impact of contact recombination and optical losses, permitting a power conversion efficiency of greater than 20% in the initial proof‐of‐concept stage. The implementation of the LiFx/Al contact mitigates the need for the costly high‐temperature phosphorus diffusion, typically implemented in such a cell design to nullify the issue of Fermi level pinning at the electron contact. The timing of this demonstration is significant, given the ongoing transition from p‐type to n‐type c‐Si solar cell architectures, together with the increased adoption of advanced PRC device structures within the c‐Si photovoltaic industry. 相似文献
103.
Deterministic seasonality can explain the evolution of alternative life history phenotypes (i.e., life history polyphenism) expressed in different generations emerging within the same year. However, the influence of stochastic variation on the expression of such life history polyphenisms in seasonal environments is insufficiently understood. Here, we use insects as a model and explore (1) the effects of stochastic variation in seasonality and (2) the life cycle on the degree of life history differentiation among the alternative developmental pathways of direct development and diapause (overwintering), and (3) the evolution of phenology. With numerical simulation, we determine the values of development (growth) time, growth rate, body size, reproductive effort, adult life span, and fecundity in both the overwintering and directly developing generations that maximize geometric mean fitness. The results suggest that natural selection favors the expression of alternative life histories in the alternative developmental pathways even when there is stochastic variation in seasonality, but that trait differentiation is affected by the developmental stage that overwinters. Increasing environmental unpredictability induced a switch to a bet‐hedging type of life history strategy, which is consistent with general life history theory. Bet‐hedging appeared in our study system as reduced expression of the direct development phenotype, with associated changes in life history phenotypes, because the fitness value of direct development is highly variable in uncertain environments. Our main result is that seasonality itself is a key factor promoting the evolution of seasonally polyphenic life histories but that environmental stochasticity may modulate the expression of life history phenotypes. 相似文献
104.
Byung-Whi Kong Kentu Lassiter Alissa Piekarski-Welsher Sami Dridi Antonio Reverter-Gomez Nicholas James Hudson Walter Gay Bottje 《PloS one》2016,11(5)
As feed represents 60 to 70% of the cost of raising an animal to market weight, feed efficiency (the amount of dry weight intake to amount of wet weight gain) remains an important genetic trait in animal agriculture. To gain greater understanding of cellular mechanisms of feed efficiency (FE), shotgun proteomics was conducted using in-gel trypsin digestion and tandem mass spectrometry on breast muscle samples obtained from pedigree male (PedM) broilers exhibiting high feed efficiency (FE) or low FE phenotypes (n = 4 per group). The high FE group had greater body weight gain (P = 0.004) but consumed the same amount of feed (P = 0.30) from 6 to 7 wk resulting in higher FE (P < 0.001). Over 1800 proteins were identified, of which 152 were different (P < 0.05) by at least 1.3 fold and ≤ 15 fold between the high and low FE phenotypes. Data were analyzed for a modified differential expression (DE) metric (Phenotypic Impact Factors or PIF) and interpretation of protein expression data facilitated using the Ingenuity Pathway Analysis (IPA) program. In the entire data set, 228 mitochondrial proteins were identified whose collective expression indicates a higher mitochondrial expression in the high FE phenotype (binomial probability P < 0.00001). Within the top up and down 5% PIF molecules in the dataset, there were 15 mitoproteome proteins up-regulated and only 5 down-regulated in the high FE phenotype. Pathway enrichment analysis also identified mitochondrial dysfunction and oxidative phosphorylation as the number 1 and 5 differentially expressed canonical pathways (up-regulated in high FE) in the proteomic dataset. Upstream analysis (based on DE of downstream molecules) predicted that insulin receptor, insulin like growth receptor 1, nuclear factor, erythroid 2-like 2, AMP activated protein kinase (α subunit), progesterone and triiodothyronine would be activated in the high FE phenotype whereas rapamycin independent companion of target of rapamycin, mitogen activated protein kinase 4, and serum response factor would be inhibited in the high FE phenotype. The results provide additional insight into the fundamental molecular landscape of feed efficiency in breast muscle of broilers as well as further support for a role of mitochondria in the phenotypic expression of FE.Funding provided by USDA-NIFA (#2013–01953), Arkansas Biosciences Institute (Little Rock, AR), McMaster Fellowship (AUS to WB) and the Agricultural Experiment Station (Univ. of Arkansas, Fayetteville). 相似文献
105.
The contrasting effects of short‐ and long‐term habitat drainage on the population dynamics of freshwater turtles in a human‐dominated landscape
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Zoey Owen‐Jones Pauline Priol Stephanie Thienpont Marc Cheylan Gabrielle Sauret Christophe Coïc Aurélien Besnard 《Freshwater Biology》2016,61(1):121-132
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108.
You Li Hisato Yagi Ezenwa Obi Onuoha Rama Rao Damerla Richard Francis Yoshiyuki Furutani Muhammad Tariq Stephen M. King Gregory Hendricks Cheng Cui Manush Saydmohammed Dong Min Lee Maliha Zahid Iman Sami Linda Leatherbury Gregory J. Pazour Stephanie M. Ware Toshio Nakanishi Elizabeth Goldmuntz Michael Tsang Cecilia W. Lo 《PLoS genetics》2016,12(2)
Heterotaxy, a birth defect involving left-right patterning defects, and primary ciliary dyskinesia (PCD), a sinopulmonary disease with dyskinetic/immotile cilia in the airway are seemingly disparate diseases. However, they have an overlapping genetic etiology involving mutations in cilia genes, a reflection of the common requirement for motile cilia in left-right patterning and airway clearance. While PCD is a monogenic recessive disorder, heterotaxy has a more complex, largely non-monogenic etiology. In this study, we show mutations in the novel dynein gene DNAH6 can cause heterotaxy and ciliary dysfunction similar to PCD. We provide the first evidence that trans-heterozygous interactions between DNAH6 and other PCD genes potentially can cause heterotaxy. DNAH6 was initially identified as a candidate heterotaxy/PCD gene by filtering exome-sequencing data from 25 heterotaxy patients stratified by whether they have airway motile cilia defects. dnah6 morpholino knockdown in zebrafish disrupted motile cilia in Kupffer’s vesicle required for left-right patterning and caused heterotaxy with abnormal cardiac/gut looping. Similarly DNAH6 shRNA knockdown disrupted motile cilia in human and mouse respiratory epithelia. Notably a heterotaxy patient harboring heterozygous DNAH6 mutation was identified to also carry a rare heterozygous PCD-causing DNAI1 mutation, suggesting a DNAH6/DNAI1 trans-heterozygous interaction. Furthermore, sequencing of 149 additional heterotaxy patients showed 5 of 6 patients with heterozygous DNAH6 mutations also had heterozygous mutations in DNAH5 or other PCD genes. We functionally assayed for DNAH6/DNAH5 and DNAH6/DNAI1 trans-heterozygous interactions using subthreshold double-morpholino knockdown in zebrafish and showed this caused heterotaxy. Similarly, subthreshold siRNA knockdown of Dnah6 in heterozygous Dnah5 or Dnai1 mutant mouse respiratory epithelia disrupted motile cilia function. Together, these findings support an oligogenic disease model with broad relevance for further interrogating the genetic etiology of human ciliopathies. 相似文献
109.
Historical and contemporary determinants of global phylogenetic structure in tropical reef fish faunas
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![点击此处可从《Ecography》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Fabien Leprieur Simona Colosio Patrice Descombes Valeriano Parravicini Michel Kulbicki Peter F. Cowman David R. Bellwood David Mouillot Loïc Pellissier 《Ecography》2016,39(9):825-835
Identifying the main determinants of tropical marine biodiversity is essential for devising appropriate conservation measures mitigating the ongoing degradation of coral reef habitats. Based on a gridded distribution database and phylogenetic information, we compared the phylogenetic structure of assemblages for three tropical reef fish families (Labridae: wrasses, Pomacentridae: damselfishes and Chaetodontidae: butterflyfishes) using the net relatedness (NRI) and nearest taxon (NTI) indices. We then related these indices to contemporary and historical environmental conditions of coral reefs using spatial regression analyses. Higher levels of phylogenetic clustering were found for fish assemblages in the Indo‐Australian Archipelago (IAA), and more particularly when considering the NTI index. The phylogenetic structure of the Pomacentridae, and to a lower extent of the Chaeotodontidae and Labridae, was primarily associated with the location of refugia during the Quaternary period. Phylogenetic clustering in the IAA may partly result from vicariance events associated with coral reef fragmentation during the glacial periods of the Quaternary. Variation in the patterns among fish families further suggest that dispersal abilities may have interacted with past habitat availability in shaping the phylogenetic structure of tropical reef fish assemblages. 相似文献
110.
Model‐based integration of observed and expert‐based information for assessing the geographic and environmental distribution of freshwater species
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Freshwater ecosystems harbor specialized and vulnerable biodiversity, and the prediction of potential impacts of freshwater biodiversity to environmental change requires knowledge of the geographic and environmental distribution of taxa. To date, however, such quantitative information about freshwater species distributions remains limited. Major impediments include heterogeneity in available species occurrence data, varying detectability of species in their aquatic environment, scarcity of contiguous freshwater‐specific predictors, and methods that support addressing these issues in a single framework. Here we demonstrate the use of a hierarchical Bayesian modeling (HBM) framework that combines disparate species occurrence information with newly‐developed 1 km freshwater‐specific predictors, to account for imperfect species detection and make fine‐grain (1 km) estimates of distributions in freshwater organisms. The approach integrates a Bernoulli suitability and a Binomial observability process into a hierarchical zero‐inflated Binomial model. The suitability process includes point presence observations, records of site visits, 1 km environmental predictors and expert‐derived species range maps integrated with a distance‐decay function along the within‐stream distance as covariates. The observability process uses repeated observations to estimate a probability of observation given that the species was present. The HBM accounts for the spatial autocorrelation in species habitat suitability projections using an intrinsic Gaussian conditional autoregressive model. We used this framework for three fish species native to different regions and habitats in North America. Model comparison shows that HBMs significantly outperformed non‐spatial GLMs in terms of AUC and TSS scores, and that expert information when appropriately included in the model can provide an important refinement. Such ancillary species information and an integrative, hierarchical Bayesian modeling framework can therefore be used to advance fine‐grain habitat suitability predictions and range size estimates in the freshwater realm. Our approach is extendable in terms of data availability and generality and can be used on other freshwater organisms and regions. 相似文献