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Cambuci (Campomanesia phaea) is a species from the Brazilian Atlantic Forest biome with a low propagation rate and is under risk of extinction. This plant has ornamental value, and its fruits have numerous nutraceutical and medicinal properties. The objective of the present study was to apply plant tissue culture techniques in its propagation. Seeds were germinated in vitro using MS and WPM medium at three pH values (5.8, 5.2, and 4.5). It was demonstrated that this species needs a reduced concentration of salts and acidic pH for its development. From in vitro twinned plants, three explant types (nodal segment, hypocotyl, and root) were evaluated under the action of the cytokinins BAP, zeatin, and kinetin at concentrations 0.0, 1.0, 2.0 and 3.0 mg L?1. The nodal segments inoculated on WPM medium containing 3% sucrose, 0.2% Phytagel®, 1.0 mg L?1 BAP at 4.5 pH showed the best formation of shoots, while the other treatments showed susceptibility to oxidation. Regenerated rooted plants showed a survival rate of 94.4% after acclimatization.
相似文献The study aims at discussing abiotic stress-related genes and metabolites in the crosstalking signal transduction pathways with emphasis to those in leaves of wild barley Hordeum spontaneum. Selected pathways involve tryptophan and target of rapamycin (or TOR) complex as core metabolites. In H. spontaneum, route towards tryptophan production seems to be mediated via anthranilate synthase acting as a master switch. Downstream auxin-related factors and proteins can secure plant’s ability to maintain proper growth rates and stress tolerance. Detected TOR in H. spontaneum participates in several signal transduction related pathways with a central role in nutrient starvation, ribosome biogenesis and protein synthesis and in standing oxidative and other abiotic stresses. TOR also participates as a master regulator of lifespan, aging and autophagy in H. spontaneum to regulate growth and maintain plant yield and its attributes under stress conditions. In conclusion, the present study emphasizes some mechanisms by which wild plant species, e.g., H. spontaneum, utilize to cope with abiotic stresses.
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