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Straus C Samara Z Fiamma MN Bautin N Ranohavimparany A Le Coz P Golmard JL Darré P Zelter M Poon CS Similowski T 《American journal of physiology. Regulatory, integrative and comparative physiology》2011,300(5):R1163-R1174
Human ventilation at rest exhibits mathematical chaos-like complexity that can be described as long-term unpredictability mediated (in whole or in part) by some low-dimensional nonlinear deterministic process. Although various physiological and pathological situations can affect respiratory complexity, the underlying mechanisms remain incompletely elucidated. If such chaos-like complexity is an intrinsic property of central respiratory generators, it should appear or increase when these structures mature or are stimulated. To test this hypothesis, we employed the isolated tadpole brainstem model [Rana (Pelophylax) esculenta] and recorded the neural respiratory output (buccal and lung rhythms) of pre- (n = 8) and postmetamorphic tadpoles (n = 8), at physiologic (7.8) and acidic pH (7.4). We analyzed the root mean square of the cranial nerve V or VII neurograms. Development and acidosis had no effect on buccal period. Lung frequency increased with development (P < 0.0001). It also increased with acidosis, but in postmetamorphic tadpoles only (P < 0.05). The noise-titration technique evidenced low-dimensional nonlinearities in all the postmetamorphic brainstems, at both pH. Chaos-like complexity, assessed through the noise limit, increased from pH 7.8 to pH 7.4 (P < 0.01). In contrast, linear models best fitted the ventilatory rhythm in all but one of the premetamorphic preparations at pH 7.8 (P < 0.005 vs. postmetamorphic) and in four at pH 7.4 (not significant vs. postmetamorphic). Therefore, in a lower vertebrate model, the brainstem respiratory central rhythm generator accounts for ventilatory chaos-like complexity, especially in the postmetamorphic stage and at low pH. According to the ventilatory generators homology theory, this may also be the case in mammals. 相似文献
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Kamal R. Dhakal Ling Gu Shivaranjani Shivalingaiah Torry S. Dennis Samara A. Morris-Bobzean Ting Li Linda I. Perrotti Samarendra K. Mohanty 《PloS one》2014,9(11)
Stimulation of specific neurons expressing opsins in a targeted region to manipulate brain function has proved to be a powerful tool in neuroscience. However, the use of visible light for optogenetic stimulation is invasive due to low penetration depth and tissue damage owing to larger absorption and scattering. Here, we report, for the first time, in-depth non-scanning fiber-optic two-photon optogenetic stimulation (FO-TPOS) of neurons in-vivo in transgenic mouse models. In order to optimize the deep-brain stimulation strategy, we characterized two-photon activation efficacy at different near-infrared laser parameters. The significantly-enhanced in-depth stimulation efficiency of FO-TPOS as compared to conventional single-photon beam was demonstrated both by experiments and Monte Carlo simulation. The non-scanning FO-TPOS technology will lead to better understanding of the in-vivo neural circuitry because this technology permits more precise and less invasive anatomical delivery of stimulation. 相似文献
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Honest error,precaution or alertness advertisement? Reactions to vertebrate pseudopredators in red‐nosed cuxiús (Chiropotes albinasus), a high‐canopy neotropical primate
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Adrian A. Barnett Tadeu de Oliveira Rafaela Fatima Soares da Silva Samara de Albuquerque Teixeira Pavel Tomanek Lucy M. Todd Sarah A. Boyle 《Ethology : formerly Zeitschrift fur Tierpsychologie》2018,124(3):177-187
Predation on primates is considered to have far‐reaching effects on the foraging and social ecology of a species. Primate species display a variety of responses to predator proximity and attack, ranging from active physical defense and mobbing, to flight and concealment. Warning calls are often given, and potentially threatening animals may be tracked, either actively or with head movements. Such behaviors take time that could be used for other activities. Accordingly, there should be strong selection to respond only to those species that represent a genuine threat. However, primates give defense‐based behaviors to non‐predator species. We tested the hypotheses that responses to pseudopredators are (i) precautionary calls made by individuals following the Dinner/Life Principle, or (ii) represent the ontogeny of species recognition. Of the species that ellicted a response from the cuxiús, 80% resembled a primate predator; 95% of the encounters that elicited a response from the cuxiús occurred when the distance between the pseudopredator and cuxiús was ≤20 m. In regard to the frequency of responses to pseudopredators, we found no difference between adults and juveniles (47.6% and 52.4%, respectively) and no differences between adult males and adult females (60% and 40% of the responses, respectively). However, reactions to pseudopredators were of shorter duration ( ± standard error (SE): 42.2 ± 15.9 s) than were reactions to actual predator species ( ± SE: 1,024.3 ± 329.1 s). There were only three instances where alarm calls were made to species that did not resemble predators, and 66.7% (N = 2) were made by adult cuxiús and only 33.3% (N = 1) were made by a juvenile cuxiú. Therefore, we found partial support for the Dinner/Life Principle hypothesis, but no support for the ontogeny hypothesis. Examination of such responses to pseudopredators in other primate and non‐primate species may help understand the evolution of such behaviors. 相似文献
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Talita O. Araújo Samara Arcanjo‐Silva Larisse Freitas‐Silva Aristéa A. Azevedo Adriano Nunes‐Nesi 《Physiologia plantarum》2016,157(2):135-146
Arsenic (As) pollution is a major environmental concern due to its worldwide distribution and high toxicity to organisms. The fern Pityrogramma calomelanos is one of the few plant species known to be able to hyperaccumulate As, although the mechanisms involved are largely unknown. This study aimed to investigate the metabolic adjustments involved in the As‐tolerance of P. calomelanos. For this purpose, ferns with five to seven fronds were exposed to a series of As concentrations. Young fronds were used for biochemical analysis and metabolite profiling using gas chromatography–mass spectrometry. As treatment increased the total concentration of proteins and soluble phenols, enhanced peroxidase activities, and promoted disturbances in nitrogen and carbon metabolism. The reduction of the glucose pool was one of the striking responses to As. Remarkable changes in amino acids levels were observed in As‐treated plants, including those related to biosynthesis of glutathione and phenols, osmoregulation and two photorespiratory intermediates. In addition, increases in polyamines levels and antioxidant enzyme activities were observed. In summary, this study indicates that P. calomelanos tolerates high concentration of As due to its capacity to upregulate biosynthesis of amino acids and antioxidants, without greatly disturbing central carbon metabolism. At extremely high As concentrations, however, this protective mechanism fails to block reactive oxygen species production, leading to lipid peroxidation and leaf necrosis. 相似文献
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Malta Priscila Gonçalves Arcanjo-Silva Samara Ribeiro Cleberson Campos Naiara Viana Azevedo Aristéa Alves 《Plant and Soil》2016,408(1-2):369-384
Plant and Soil - Rudgea viburnoides (Cham.) Benth. is an aluminum (Al) hyperaccumulator species native to the Brazilian Cerrado and can be found on soils with different fertilities and Al... 相似文献
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Lysosomal biogenesis and function is critical for necrotic cell death in Caenorhabditis elegans
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Necrotic cell death is defined by distinctive morphological characteristics that are displayed by dying cells (Walker, N.I., B.V. Harmon, G.C. Gobe, and J.F. Kerr. 1988. Methods Achiev. Exp. Pathol. 13:18-54). The cellular events that transpire during necrosis to generate these necrotic traits are poorly understood. Recent studies in the nematode Caenorhabditis elegans show that cytoplasmic acidification develops during necrosis and is required for cell death (Syntichaki, P., C. Samara, and N. Tavernarakis. 2005. Curr. Biol. 15:1249-1254). However, the origin of cytoplasmic acidification remains elusive. We show that the alkalization of endosomal and lysosomal compartments ameliorates necrotic cell death triggered by diverse stimuli. In addition, mutations in genes that result in altered lysosomal biogenesis and function markedly affect neuronal necrosis. We used a genetically encoded fluorescent marker to follow lysosome fate during neurodegeneration in vivo. Strikingly, we found that lysosomes fuse and localize exclusively around a swollen nucleus. In the advanced stages of cell death, the nucleus condenses and migrates toward the periphery of the cell, whereas green fluorescent protein-labeled lysosomal membranes fade, indicating lysosomal rupture. Our findings demonstrate a prominent role for lysosomes in cellular destruction during necrotic cell death, which is likely conserved in metazoans. 相似文献
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Regulation of K-Cl cotransport during reticulocyte maturation and erythrocyte aging in normal and sickle erythrocytes 总被引:3,自引:0,他引:3
The age/density-dependent decrease in K-Cl cotransport (KCC), PP1 and PP2A activities in normal and sickle human erythrocytes, and the effect of urea, a known KCC activator, were studied using discontinuous, isotonic gradients. In normal erythrocytes, the densest fraction (d 33.4 g/dl) has only about 5% of the KCC and 4% of the membrane (mb)-PP1 activities of the least-dense fraction (d 24.7 g/dl). In sickle and normal erythrocytes, density-dependent decreases for mb-PP1 activity were similar (d50% 28.1 ± 0.4 vs. 27.2 ± 0.2 g/dl, respectively), whereas those for KCC activity were not (d50% 31.4 ± 0.9 vs. 26.8 ± 0.3 g/dl, respectively, P = 0.004). Excluding the 10% least-dense cells, a very tight correlation exists between KCC and mb-PP1 activities in normal (r2 = 0.995) and sickle erythrocytes (r2 = 0.93), but at comparable mb-PP1 activities, KCC activity is higher in sickle erythrocytes, suggesting a defective, mb-PP1-independent KCC regulation. In normal, least-dense but not in densest cells, urea stimulates KCC (two- to fourfold) and moderately increases mb-PP1 (2040%). Thus mb-PP1 appears to mediate part of urea-stimulated KCC activity. phosphorylation; protein phosphatase; urea; cell size; density 相似文献
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Dorta DJ Leite S DeMarco KC Prado IM Rodrigues T Mingatto FE Uyemura SA Santos AC Curti C 《Journal of inorganic biochemistry》2003,97(3):251-257
Cadmium is a very important environmental toxicant, the cytotoxicity mechanism of which is likely to involve mitochondria as a target. In the present study we addressed the cause/effect relationship between the multiple cadmium-induced responses involving the mitochondrial energetic and oxidative status. Assays were performed with succinate-energized rat liver mitochondria incubated with 5 microM CdCl(2) for 0-25 min, in the absence or presence, respectively, of N-ethylmaleimide (NEM), butylhydroxytoluene (BHT), ruthenium red (RR), and cyclosporine A+ADP. A sequence of events accounting for cadmium-induced mitochondrial impairment is proposed, beginning with an apparent interaction of Cd(2+) with specific protein thiols in the mitochondrial membrane, which stimulates the cation's uptake via the Ca(2+) uniporter, and is followed by the onset of mitochondrial permeability transition (MPT); both effects dissipate the transmembrane electrical potential (Deltapsi), causing uncoupling, followed by an early depression of mitochondrial ATP levels. The respiratory chain subsequently undergoes inhibition, generating reactive oxygen species which together with iron mobilized by the cation, cause late, gradual mitochondrial membrane lipid peroxidation. 相似文献