排序方式: 共有124条查询结果,搜索用时 15 毫秒
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Zahra Javanmardi Mahmoud Mahmoudi Houshang Rafatpanah Zahra Rezaieyazdi Abbas Shapouri-Moghaddam Parisa Ahmadi Samaneh Mollazadeh Nafiseh Sadat Tabasi Seyed-Alireza Esmaeili 《Cell biochemistry and function》2024,42(2):e3981
Systemic lupus erythematosus (SLE) is known as an autoimmune disorder that is characterized by the breakdown of self-tolerance, resulting in disease onset and progression. Macrophages have been implicated as a factor in the development of SLE through faulty phagocytosis of dead cells or an imbalanced M1/M2 ratio. The study aimed to investigate the immunomodulatory effects of Lactobacillus delbrueckii and Lactobacillus rhamnosus on M1 and M2 macrophages in new case lupus patients. For this purpose, blood monocytes were collected from lupus patients and healthy people and were cultured for 5 days to produce macrophages. For 48 h, the macrophages were then cocultured with either probiotics or lipopolysaccharides (LPS). Flow cytometry and real-time polymerase chain reaction were then used to analyze the expression of cluster of differentiation (CD) 14, CD80, and human leukocyte antigen – DR (HLADR) markers, as well as cytokine expression (interleukin [IL]1-β, IL-12, tumor necrosis factor α [TNF-α], IL-10, and transforming growth factor beta [TGF-β]). The results indicated three distinct macrophage populations, M0, M1, and M2. In both control and patient-derived macrophage-derived monocytes (MDMs), the probiotic groups showed a decrease in CD14, CD80, and HLADR expression compared to the LPS group. This decrease was particularly evident in M0 and M2 macrophages from lupus patients and M1 macrophages from healthy subjects. In addition, the probiotic groups showed increased levels of IL-10 and TGF-β and decreased levels of IL-12, IL1-β, and TNF-α in MDMs from both healthy and lupus subjects compared to the LPS groups. Although there was a higher expression of pro-inflammatory cytokines in lupus patients, there was a higher expression of anti-inflammatory cytokines in healthy subjects. In general, L. delbrueckii and L. rhamnosus could induce anti-inflammatory effects on MDMs from both healthy and lupus subjects. 相似文献
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Majid Ahmadi Mahnaz Ghaebi Samaneh Abdolmohammadi-Vahid Sanaz Abbaspour-Aghdam Kobra Hamdi Sedigheh Abdollahi-Fard Shahla Danaii Parisa Mosapour Ladan Koushaeian Sanam Dolati Reza Rikhtegar Farnaz Dabiri Oskouei Leili Aghebati-Maleki Mohammad Nouri Mehdi Yousefi 《Journal of cellular physiology》2019,234(6):9428-9437
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Plasmonics - Excitation of THz surface plasmons due to the scattering of transverse magnetic (TM) wave by an elliptical plasma antenna consisting of a metallic rod with dielectric coating is... 相似文献
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Molecular and Cellular Biochemistry - MicroRNAs (miRNAs) are important molecules which implicated in various processes, such as differentiation, development, cell survival, cell apoptosis and also... 相似文献
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Masoumeh Douraghi Samaneh Saberi Kashani Hojjat Zeraati Maryam Esmaili Akbar Oghalaie Marjan Mohammadi 《Current microbiology》2010,60(4):254-262
Helicobacter pylori, a microaerophilic fastidious bacterium, has been cultured on various plating and broth media since its discovery. Although
the agar media can be sufficient for the identification, typing, and antibiotic resistance studies, no secretory antigen of
H. pylori can be evaluated in such media. Thus, satisfactory growth of H. pylori in liquid culture which is needed for analysis of secretory proteins without the presence of interfering agents is in demand.
We assessed the impact of β-cyclodextrin, Fetal Bovine Serum (FBS), and charcoal as supplements for H. pylori growth. Furthermore, we aimed to identify the most favorable supplement that supports the secretion of the dominant secretory
protein, vacuolating cytotoxin (VacA). Five clinical strains were cultured on broth media and the growth, viability, morphology,
and protein content of each strain were determined. Our results revealed that β-cyclodextrin supports the growth rate, viability,
and cell lysate protein content to the extent similar to FBS. Application of β-cyclodextrin is found to postpone spiral to
coccoid conversion up to 72 h of incubation. Although FBS supports a higher VacA protein content, presence of interfering
macromolecules in FBS questions its utility particularly for purposes of studying extra cellular proteins such as VacA. This
study recommends further application of β-cyclodextrin as a culture supplement with the potential capacity in neutralizing toxic compounds and flourishing the secretion
of H. pylori proteins without addition of interfering proteins. 相似文献
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Zarghi A Kakhgi S Hadipoor A Daraee B Dadrass OG Hedayati M 《Bioorganic & medicinal chemistry letters》2008,18(4):1336-1339
A group of 1,3-diarylurea derivatives, possessing a methylsulfonyl pharmacophore at the para-position of the N-1 phenyl ring, in conjunction with a N-3 substituted-phenyl ring (4-F, 4-Cl, 4-Me, 4-OMe), were designed and synthesized for evaluation as selective cyclooxygenase-2 (COX-2) inhibitors. In vitro COX-1/COX-2 isozyme inhibition structure-activity studies identified 1-(4-methylsulfonylphenyl)-3-(4-methoxyphenyl) urea (4e) as a potent COX-2 inhibitor (IC(50)=0.11 microM) with a high COX-2 selectivity index (SI=203.6) comparable to the reference drug celecoxib (COX-2 IC(50)=0.06 microM; COX-2 SI=405). The structure-activity data acquired indicate that the urea moiety constitutes a suitable scaffold to design new acyclic 1,3-diarylurea derivatives with selective COX-2 inhibitory activity. 相似文献
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Samaneh Fatemikia Jaber Karimi Ayatallah Saeedizadeh Aziz Sheikhi Gorjan 《Archives Of Phytopathology And Plant Protection》2013,46(8):1008-1014
Two-spotted spider mite, Tetranychus urticae Koch is the major pest of various plants worldwide. Now the control is dependent on the use of chemical pesticides. Plant compounds are recently known as biopesticides. Essential oil of Elettaria cardamomum was researched on repellent and oviposition inhibition of T. urticae. The LC50 values of fumigant toxicity of this oil on adults and eggs of the two spotted spider mite were 7.26 and 8.82?μL/L air, respectively. Also LT50 value of essential oil at 45?μL/L air was 23.86?h and LT50 value of essential oil at 60?μL/L air was 9.01?h. In addition, different concentrations of the essential oil of E. cardamomum significantly affected oviposition deterrence and repellency of adults. The results of this study indicated that essential oil of E. cardamomum may be considered as a biopesticide to control two spotted spider mites. 相似文献
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