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51.
Active sweat gland distribution in Caingang Indians 总被引:1,自引:0,他引:1
On a small sample of male Caingang Indians in Southern Brazil was counted the number of sweat glands active under thermal stimulation. The results at nine sites over the body are compared with those from samples of other races, the factors affecting the findings are discussed, and it is concluded that the Caingang results show no racial difference in the number of active glands, or in the gradient of their distribution over the body. 相似文献
52.
Francisco M. Salzano 《Chromosoma》1955,7(1):39-50
Summary Studies of chromosomal polymorphism in natural populations of D. guaramunu and D. griseolineata in Rio Grande do Sul, Brazil, confirmed and extended previous findings in these species. The more common D. guaramunu is much more polymorphic than its closest but less common relative D. griseolineata. In the former species, the frequencies of four inversions showed differences from locality to locality, and five inversions of the same species showed temporal changes in the population of Bexiga. It was not possible to correlate the differences with any particular factor in the environment, though the temporal changes were perhaps due to seasonal climatic cycles.Inversion heterosis was not evident in either species — instead, inversion Ee of D. guaramunu was found to be less frequent than expected on the basis of the Hardy-Weinberg equilibrium in the localities of Emboaba, Bexiga and Feliz. The implications of these findings are discussed.Fellow of the Conselho Nacional de Pesquisas. 相似文献
53.
Divergent Human Y-Chromosome Microsatellite Evolution Rates 总被引:5,自引:0,他引:5
Denise R. Carvalho-Silva Fabrício R. Santos Mara H. Hutz Francisco M. Salzano Sérgio D.J. Pena 《Journal of molecular evolution》1999,49(2):204-214
In this work, we analyze several characteristics influencing the low variability of the microsatellite DYS19 in the major founder Amerindian Y chromosome lineage containing the point mutation DYS199-T. Variation of DYS19 was compared with that of five other Y-linked tetranucleotide repeat loci (DYS389A, DYS389B, DYS390, DYS391, and DYS393) in the DYS199-T lineage. All the other microsatellites showed significantly higher levels of variability than DYS19 as measured by gene diversity and repeat number variance. Moreover, we had previously shown that DYS19 had high diversity in Brazilians and in several other populations worldwide. Thus, the slow DYS19 evolution in the DYS199-T lineage seems to be both locus and allele specific. To understand the slow DYS19 evolutionary rate, the microsatellite loci were compared according to their mapping on the Y chromosome and also on the basis
of structural aspects such as the base composition of the repeat motif and flanking regions and the degree of perfection and
size (repeat number) of the variable blocks. The only observed difference that might be related to the low DYS19 variability is its small average number of repeats, a value expected to be closer to the founder DYS19 allele in the DYS199-T lineage. These data were also compared to other derived Y lineages. The Tat-C lineage displayed a lower DYS19 variability correlated to a small average repeat number, while in the DYS234-G lineage, a high DYS19 variability was found associated to a larger average repeat number. This approach reveals that evolution of Y microsatellites
in lineages defined by slowly evolving markers, such as point mutations, can be greatly influenced by the size (number of
repeats of the variable block) of the founder allele in each microsatellite locus. Thus lineage-dating methods using microsatellite
variation should be practiced with great care.
Received: 7 November 1998 / Accepted: 9 April 1999 相似文献
54.
Maria Ctira Bortolini Francisco Mauro Salzano Marco Antonio Zago Wilson Araujo Da Silva Tania De Azevedo Weimer 《American journal of physical anthropology》1997,103(2):147-156
Sequence data from the first hypervariable segment of the mitochondrial DNA control region of 124 subjects belonging to three African-Brazilian and three Brazilian Indian populations were compared with information related to 12 protein genetic loci from 601 persons living in the same localities. There is high diversity among the mtDNA sites, and the most variable in one ethnic group are not the most variable in the other. No differences in gene diversity between populations within ethnic groups were observed, but the Indians showed a reduced variability. Much more interpopulation variation was observed in the mtDNA data than in the protein set. The relationships obtained for the six populations, however, are the same regardless whether mtDNA or protein loci are considered. African-Brazilians from Porto Alegre and Salvador, situated 3,000 km apart, are more similar to each other than both are to Paredão, despite the geographical proximity between Porto Alegre and Paredão, which are just 50 km apart. The tree topology in relation to the three Indians groups, on the other hand, is that expected when languages, culture, and geography are considered. Am J Phys Anthropol 103:147–156, 1997. © 1997 Wiley-Liss, Inc. 相似文献
55.
56.
Gunnel Hellgren Björn Andersson Andreas FM Nierop Jovanna Dahlgren Ze'ev Hochberg Kerstin Albertsson-Wikland 《Proteome science》2008,6(1):35
Background
The broad range in growth observed in short prepubertal children receiving the same growth hormone (GH) dose is due to individual variation in GH responsiveness. This study used a pharmaco-proteomic approach in order to identify novel biomarkers that discriminate between short non-GH-deficient (GHD) children who show a good or poor growth response to GH treatment. 相似文献57.
Björn Andersson Gunnel Hellgren Andreas FM Nierop Ze'ev Hochberg Kerstin Albertsson-Wikland 《Proteome science》2009,7(1):40-12
Background
The broad range in growth observed in response to growth hormone (GH) treatment is mainly caused by individual variations in both GH secretion and GH sensitivity. Individual GH responsiveness can be estimated using evidence-based models that predict the response to GH treatment; however, these models can be improved. High-throughput proteomics techniques can be used to identify proteins that may potentially be used as variables in such models in order to improve their predictive ability. Previously we have reported that proteomic analyses can identify biomarkers that discriminate between short prepubertal children with idiopathic short stature (ISS) who show good or poor growth in response to GH treatment. In this study we used a pharmaco-proteomic approach to identify novel factors that correlate with the growth response to GH treatment in prepubertal children who are short due to GH deficiency or ISS. The study included 128 short prepubertal children receiving GH treatment, of whom 39 were GH-deficient and 89 had ISS. Serum protein expression profiles at study start and after 1 year of GH treatment were analyzed using SELDI-TOF. Cross-validated regression and random permutation analyses were performed to identify significant correlations between protein expression patterns and the 2-year growth response to GH treatment. 相似文献58.
Sina Babazadeh Matthew L Broadhead John L Slavin Peter FM Choong 《International Seminars in Surgical Oncology : ISSO》2009,6(1):18
A presacral mass can present a diagnostic dilemma for the surgical oncologist. Differential diagnoses include congenital causes such as teratoma or chordoma, neurological causes such as neurilemoma or neurofibroma or other malignancies such as lymphoma or sarcoma. Diagnosis usually requires imaging such as CT and MRI and tissue biopsy. We present an unusual cause of a presacral mass being extramedullary haematopoiesis, found incidentally in a 71 year old female. Extramedullary haematopoiesis is defined as the production of myeloid and erythroid elements outside of the bone-marrow. This diagnosis is extremely rare in the presacral area especially in a patient with no haematological abnormalities. A review of the literature is presented. 相似文献
59.
Salzano A Kochiashvili N Nergadze SG Khoriauli L Smirnova A Ruiz-Herrera A Mondello C Giulotto E 《DNA Repair》2009,8(1):19-28
Gene amplification, a key mechanism for oncogene activation and drug resistance in tumour cells, involves the generation and joining of DNA double-strand breaks. Amplified DNA can be carried either on intra-chromosomal arrays or on extra-chromosomal elements (double minutes). We previously showed that, in rodent cells deficient in DNA-PKcs, intra-chromosomal amplification is significantly enhanced. In the present work, we studied gene amplification in human HeLa cell lines in which the expression of the DNA-PKcs gene was constitutively inhibited by shRNAs. These cell lines showed an increased sensitivity to ionizing radiations, an enhanced frequency of chromosomal aberrations and an increased rate of occurrence of methotrexate resistant colonies compared to the control cell lines (6-18 times). The main mechanism of resistance to methotrexate was extra-chromosomal amplification of the dihydrofolate reductase gene. These results indicate that, in human cells, inhibition of DNA-PKcs gene expression favours gene amplification occurring via the production of double minutes. In addition, they show that cell lines constitutively expressing shRNAs are good model systems to study the role of specific functions in gene amplification. 相似文献
60.
Blaiotta G Ercolini D Mauriello G Salzano G Villani F 《Systematic and applied microbiology》2004,27(6):696-702
Rapid and reliable identification of Staphylococcus (S.) equorum was achieved by species-specific PCR assays. A set of primers targeting the manganese-dependent superoxide dismutase (sodA) gene of S.equorum was designed. Species-specificity of the primer set was evaluated by using a total of 112 strains (including 27 reference strains of the DSM collection), representing 26 different species of the genus Staphylococcus, 3 species of the genus Kocuria, and different strains of Macrococcus caseolyticus. By using primers SdAEqF and SdAEqR the expected PCR fragment was obtained only when DNA from S. equorum strains was used as template. The rapidity (about 4 h from DNA isolation to results) and reliability of the PCR procedures established suggests that the method may be profitably applied for specific detection and identification of S. equorum strains. 相似文献