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91.
Relationships between Dicer 1 polymorphism and expression levels in the etiopathogenesis of preeclampsia
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92.
IDO‐Expressing Fibroblasts Protect Islet Beta Cells From Immunological Attack and Reverse Hyperglycemia in Non‐Obese Diabetic Mice
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93.
Minoo Yaghmaei Saeedeh Salimi Lida Namazi Farzaneh Farajian-Mashhadi 《Genetics and molecular biology》2015,38(4):444-449
The aim of present study was to investigate the role of the X-ray repair
cross-complementing protein1 (XRCC1) and Tumor
protein p53 (Tp53) polymorphisms in Uterine Leiomyoma
(UL) susceptibility in southeastern Iran. This case control study was performed on
139 women with UL and 149 age, BMI and ethnicity matched healthy women. All women
were genotyped for the XRCC1 Arg399Gln, XRCC1
Arg194Trp and Tp53 Arg72Pro polymorphisms. The frequency of
Tp53 72 Pro/Pro genotype was significantly higher in UL women
compared to controls. The risk of UL was 1.5 fold higher in women with the Pro/Pro
genotype (OR, 1.5 [95% CI, 1.1 to 2.1], p = 0.012). Moreover, the frequency of the
Pro allele was significantly higher in the UL women. Although the frequency of
XRCC1 Arg399Gln genotypes did not significantly differ between UL
and control groups before adjusting for age, there was an association between the
XRCC1 Arg/Gln genotype and UL after adjusting for age (OR, 1.8
[95% CI, 1.1 to 3]). No association was observed between the XRCC1
Arg194Trp polymorphism and UL. The Pro/Pro genotype of Tp53 Arg72Pro
polymorphism was associated with UL susceptibility. In addition, the
XRCC1 Arg/Gln genotype was associated with increased risk of UL
after adjusting for age. 相似文献
94.
Teimoori Batool Moradi-shahrebabak Maryam Razavi Maryam Rezaei Mahnaz Harati-Sadegh Mahdiyeh Salimi Saeedeh 《Molecular biology reports》2019,46(6):6099-6104
Molecular Biology Reports - Preeclampsia (PE) is a serious pregnancy complication whose etiology is not fully understood. However, previous reports have suggested that oxidative stress and genetic... 相似文献
95.
Moradpour F. Salimi Z. Zarei F. Pourmotabbed A. Nedaei S. E. Veisi M. 《Neurophysiology》2022,53(2):93-100
Neurophysiology - In recent years, the misuse of nandrolone decanoate (ND) among non-athletes, especially adolescent males, has become a growing problem due to the adverse effects of this drug. In... 相似文献
96.
Mehrdad Gholami Alireza Salimi Chirani Mona Moshiri Mansour Sedighi Abazar Pournajaf Masoud Tohidfar Gholamreza Irajian 《Reports of Biochemistry & Molecular Biology》2015,4(1):50-59
Background:
Neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5–15% of adults. The aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type IV pilus biogenesis protein antigen (ACP-MIP-PilQ) from N. meningitidis serogroup B strain.Methods:
Biochemical properties and multiple alignments were predicted by appropriate web servers. Secondary molecular structures were predicted based on Chou and Fasman, Garnier-Osguthorpe-Robson, and Neural Network methods. Tertiary modeling elucidated conformational properties of the chimeric protein. Proteasome cleavage and transporter associated with antigen processing (TAP) binding sites, and T- and B-cell antigenic epitopes, were predicted using bioinformatic web servers.Results:
Based on our in silico and immunoinformatics analyses, the ACP-MIP-PilQ protein (AMP) can induce high-level cross-strain bactericidal activity. In addition, several immune proteasomal cleavage sites were detected. The 22 epitopes associated with MHC class I and class II (DR) alleles were confirmed in the AMP. Thirty linear B-cell epitopes as antigenic regions were predicted from the full-length protein.Conclusion:
All predicted properties of the AMP indicate it could be a good candidate for further immunological in vitro and in vivo studies.Key Words: Chimeric protein, In silico, Neisseria meningitides, serogroup B, Vaccine 相似文献97.
Mohammad Majidi Saeedreza Pakzad Maryam Salimi Abdolnaser Azadbakht Saieh Hajighasemlou Moein Amoupour Zeinab Nokhbedehghan Shahin Bonakdar Koushan Sineh Sepehr Narendra Pal Singh Chauhan Mazaher Gholipourmalekabadi 《Biotechnology and bioengineering》2023,120(12):3638-3654
Mesenchymal stem cells and macrophages (MQ) are two very important cells involved in the normal wound healing process. It is well understood that topological cues and mechanical factors can lead to different responses in stem cells and MQ by influencing their shape, cytoskeleton proliferation, migration, and differentiation, which play an essential role in the success or failure of biomaterial implantation and more importantly wound healing. On the other hand, the polarization of MQ from proinflammatory (M1) to prohealing (M2) phenotypes has a critical role in the acceleration of wound healing. In this study, the morphology of different MQ subtypes (M0, M1, and M2) was imprinted on a silicon surface (polydimethylsiloxane [PDMS]) to prepare a nano-topography cell-imprinted substrate with the ability to induce anti-inflammatory effects on the mouse adipose-derived stem cells (ADSCs) and RAW264.7 monocyte cell line (MO). The gene expression profiles and flow cytometry of MQ revealed that the cell shape microstructure promoted the MQ phenotypes according to the specific shape of each pattern. The ELISA results were in agreement with the gene expression profiles. The ADSCs on the patterned PDMS exhibited remarkably different shapes from no-patterned PDMS. The MOs grown on M2 morphological patterns showed a significant increase in expression and section of anti-inflammatory cytokine compared with M0 and M1 patterns. The ADSCs homing in niches heavily deformed the cytoskeletal, which is probably why the gene expression and phenotype unexpectedly changed. In conclusion, wound dressings with M2 cell morphology-induced surfaces are suggested as excellent anti-inflammatory and antiscarring dressings. 相似文献
98.
Azam Salimi Mirle Schemionek-Reinders Michael Huber Margherita Vieri John B. Patterson Julia Alten Tim H. Brümmendorf Behzad Kharabi Masouleh Iris Appelmann 《Journal of cellular and molecular medicine》2023,27(21):3363-3377
Activating point mutations of the RAS gene act as driver mutations for a subset of precursor-B cell acute lymphoblastic leukaemias (pre-B ALL) and represent an ambitious target for therapeutic approaches. The X box-binding protein 1 (XBP1), a key regulator of the unfolded protein response (UPR), is critical for pre-B ALL cell survival, and high expression of XBP1 confers poor prognosis in ALL patients. However, the mechanism of XBP1 activation has not yet been elucidated in RAS mutated pre-B ALL. Here, we demonstrate that XBP1 acts as a downstream linchpin of the IL-7 receptor signalling pathway and that pharmacological inhibition or genetic ablation of XBP1 selectively abrogates IL-7 receptor signalling via inhibition of its downstream effectors, JAK1 and STAT5. We show that XBP1 supports malignant cell growth of pre-B NRASG12D ALL cells and that genetic loss of XBP1 consequently leads to cell cycle arrest and apoptosis. Our findings reveal that active XBP1 prevents the cytotoxic effects of a dual PI3K/mTOR pathway inhibitor (BEZ235) in pre-B NRASG12D ALL cells. This implies targeting XBP1 in combination with BEZ235 as a promising new targeted strategy against the oncogenic RAS in NRASG12D-mutated pre-B ALL. 相似文献
99.
D. Jahantigh A. Moghtaderi M. Narooie-Nejad M. Mousavi M. Moossavi S. Salimi M. Mohammadoo-Khorasani 《Russian Journal of Genetics》2017,53(1):147-152
The DNA damage has considerably raised in active MS lesions compared to normal brains, indicating the possible role of DNA repairing genes in MS. In the current study, we sought to highlight the association between genetic polymorphisms of XRCC5 and XRCC6 genes, involved in Double Strand Breaks (DSBs) repair, and MS susceptibility. A total of 235 Iranian individuals; including 113 MS patients and 122 healthy controls were participated in this study. They were genotyped for the XRCC5 VNTR polymorphism by polymerase chain reaction (PCR). The genotype analysis of the XRCC6–61C>G polymorphism was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The genotypic frequency of 2R/2R in the XRCC5 VNTR polymorphism was significantly higher in MS patients than controls (p = 0.048). The frequency of individuals with 2R allele was statistically significant in MS patients compared to controls (p = 0.041). Moreover, the frequency of 2R allele of the XRCC5 VNTR polymorphism was found to be significantly difference between MS patients and healthy groups (p = 0.003). The present study suggests that the presence of 2R allele in XRCC5 VNTR gene polymorphism may be a genetic risk factor for MS susceptibility in Iranian population. 相似文献
100.
Fe(3)O(4) magnetic nanoparticles were in situ loaded on the surface of multiwalled carbon nanotubes (MWCNTs) by a simple coprecipitation procedure. The resulting Fe(3)O(4)/MWCNTs nanocomposite brings new capabilities for electrochemical sensing by combining the advantages of Fe(3)O(4) magnetic nanoparticles and MWCNTs. It was found that Fe(3)O(4) has redox properties similar to those of frequently used mediators used for electron transfer between NADH and electrode. The cyclic voltammetric results indicated the ability of Fe(3)O(4)/MWCNTs modified GC electrode to catalyze the oxidation of NADH at a very low potential (0.0 mV vs. Ag/AgCl) and subsequently, a substantial decrease in the overpotential by about 650 mV compared with the bare GC electrode. The catalytic oxidation current allows the stable and selective amperometric detection of NADH at an applied potential of 0.0 mV (Ag/AgCl) with a detection limit of 0.3 μM and linear response up to 300 μM. This modified electrode can be used as an efficient transducer in the design of biosensors based on coupled dehydrogenase enzymes. Lactate dehydrogenase (LDH) and NAD(+) were subsequently immobilized onto the Fe(3)O(4)/MWCNTs nanocomposite film by covalent bond formation between the amine groups of enzyme or NAD(+) and the carboxylic acid groups of the Fe(3)O(4)/MWCNT film. Differential pulse voltammetric detection of lactate on Fe(3)O(4)/MWCNT/LDH/NAD(+) modified GC electrode gives linear responses over the concentration range of 50-500 μM with the detection limit of 5 μM and sensitivity of 7.67 μA mM(-1). Furthermore, the applicability of the sensor for the analysis of lactate concentration in human serum samples has been successfully demonstrated. 相似文献