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Salimi A Yousefi F Ghollasi M Daneshjou S Tavoli H Ghobadi S Khajeh K 《Journal of microbiology and biotechnology》2012,22(8):1077-1083
Previously, an extracellular α-amylase (BKA) had been purified from the culture of Bacillus sp. KR8104. Subsequently, the crystal structure of the active enzyme revealed a 422 amino acids polypeptide. In this study, the bka was cloned into E. coli, which encoded a polypeptide of 659 amino acids including two additional fragments: one 44 residues N-terminal fragment and another 193 residues C-terminal fragment. In order to investigate the role of the C-terminal fragment, two constructs with and without this region [BKAΔ(N44) and BKAΔ(N44C193)] were designed and expressed in E. coli BL21. The optimum pH, thermal stability, and the end-products of starch hydrolysis were found to be similar in both constructs. The Km and V(max) values for BKAΔ(N44) were lower than BKAΔ(N44C193), using either starch or ethylidene-blocked 4-nitrophenylmaltoheptaoside as a substrate. 相似文献
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Spániková A Simoncíková P Ravingerová T Pechánová O Barancík M 《Molecular and cellular biochemistry》2008,312(1-2):113-120
The aim of the present study was to characterize the effects of chronic nitric oxide synthase (NOS) inhibition on the alterations
of regulatory myocardial proteins of intracellular signaling pathways (mitogen-activated protein kinase (MAPK) and Akt kinase
cascades) and matrix metalloproteinases (MMP). Chronic NO deficiency (NOD) was induced by NG-nitro-L-arginine methyl ester (L-NAME, 40mg/kg/day, 4 weeks). Protein levels and activation of protein kinases were determined
using specific antibodies, activities of MMP were analyzed by zymography in gels containing gelatin as a substrate. The development
of NOD was associated with decreased activation of endothelial NOS (eNOS) and down-regulation of protein level of inducible
NOS (iNOS). Investigation of kinase pathways revealed that the activation of extracellular signal-regulated kinases (ERK)
and the levels of upstream activators of ERK (aFGF, H-Ras) were decreased after L-NAME treatment. Western blot analysis revealed
that chronic application of L-NAME also decreased the activation of Akt kinase as compared with control hearts. Study of MMPs
showed that in L-NAME-treated rat hearts activities of tissue MMP-2 were decreased. It is concluded that development of NOD
resulted in inhibition of ERK and Akt kinase pathways and these changes suggest the involvement of these cascades in responses
of myocardium to NOD. The results also point to the possible relationship between ERK and Akt kinase pathways and activation
of eNOS and/or MMP-2.
Anna Špániková and Petra Šimončíková have contributed equally to the study. 相似文献
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Behnam Sadeghi Elham Roshandel Ali Pirsalehi Sepide Kazemi Ghazaleh Sankanian Mohammad Majidi Maryam Salimi Nasser Aghdami Hoda Sadrosadat Sarvenaz Samadi Kochaksaraei Farshid Alaeddini Olle Ringden Abbas Hajifathali 《Journal of cellular and molecular medicine》2021,25(22):10554-10564
Acute respiratory distress syndrome (ARDS) is the most common cause of death in COVID-19 patients. The cytokine storm is the main driver of the severity and magnitude of ARDS. Placenta-derived decidua stromal cells (DSCs) have a stronger immunosuppressive effect than other sources of mesenchymal stromal cells. Safety and efficacy study included 10 patients with a median age of 50 (range 14–68) years with COVID-19-induced ARDS. DSCs were administered 1–2 times at a dose of 1 × 106/kg. End points were safety and efficacy by survival, oxygenation and effects on levels of cytokines. Oxygenation levels increased from a median of 80.5% (range 69–88) to 95% (range 78–99) (p = 0.012), and pulmonary infiltrates disappeared in all patients. Levels of IL-6 decreased from a median of 69.3 (range 35.0–253.4) to 11 (range 4.0–38.3) pg/ml (p = 0.018), and CRP decreased from 69 (range 5–169) to 6 (range 2–31) mg/ml (p = 0.028). Two patients died, one of a myocardial infarction and the other of multiple organ failure, diagnosed before the DSC therapy. The other patients recovered and left the intensive care unit (ICU) within a median of 6 (range 3–12) days. DSC therapy is safe and capable of improving oxygenation, decreasing inflammatory cytokine level and clearing pulmonary infiltrates in patients with COVID-19. 相似文献
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The association of the placental MTHFR 3′‐UTR polymorphisms,promoter methylation,and MTHFR expression with preeclampsia
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Parvaneh Naserzadeh Seyed Alireza Mortazavi Khadijeh Ashtari Ahmad Salimi Mehdi Farokhi Jalal Pourahmad 《Journal of biochemical and molecular toxicology》2018,32(6)
Silk fibroin nanoparticles (SFNPs) as a natural polymer have been utilized in biomedical applications such as suture, tissue engineering‐based scaffolds, and drug delivery carriers. Since there is little data regarding the toxicity effects on different cells and tissues, we aimed to determine the toxicity mechanisms of SFNPs on human lymphocytes and monocytes based on reliable methods. Our results showed that SFNPs (0.5, 1, and 2 mg/mL) induced oxidative stress via increasing reactive oxygen species production, mitochondrial membrane potential (?Ψ) collapse, which was correlated to cytochrome c release and Adenosine diphosphate (ADP)/Adenosine tri phosphate (ATP) ratio increase as well as lysosomal as another toxicity mechanism, which led to cytosolic release of lysosomal digestive proteases, phosphor lipases, and apoptosis signaling. Taken together, these data suggested that SFNPs toxicity was associated with mutual mitochondrial/lysosomal cross‐talk and oxidative stress on human lymphocytes and monocytes with activated apoptosis signaling. 相似文献
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