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311.
Nega Berhane Rabinder Chandera Sobti Shiferaw Melesse Salih Abdul Mahdi Afework Kassu 《Molecular biology reports》2012,39(12):11125-11130
Prostate cancer (PCa) is the most common noncutaneous cancer among men, accounting for 10?% of male cancer-related deaths worldwide. The etiology of PCa is largely unknown, although multiple environmental and lifestyle factors such as ultraviolet irradiation, smoking, and diet might increase the risk of the disease. Risk of disease varies most prominently with age, ethnicity, family history, and diet. The multifunctional cytokine tumor necrosis factor alpha (TNF-α) has an important role in the pathogenesis of inflammatory, autoimmune and malignant diseases. In this case control study 150 Prostate cancer patients and 150 age matched benign prostate hyperplasia (BPH) and equal number of healthy control groups were involved. The aim of this study was to analyze the effect of TNF-α-308 (G/A) polymorphism on risk of prostate cancer on north Indian prostate cancer patients. The polymerase chain reaction (PCR) technique was utilized to genotype TNF-α-308 (G/A) polymorphism. The present study showed statistically significant increased risk of prostate cancer among individuals that carried the A allele of TNF-α-308 gene (OR?=?1.81, 95?% CI 1.00–3.481, p?=?0.03). 相似文献
312.
Internodal explants from etiolated `Royal Gala' apple shoots were compared with those from non-etiolated shoots for frequency
of shoot organogenesis and for efficiency of β-glucuronidase (GUS) expression after cocultivation of explants with Agrobacterium tumefaciens strain EHA105 (p35SGUSint). First (youngest) internodal explants from etiolated shoots produced 2-, 8- and 73-fold numbers
of shoots compared to second, third, and fourth internodal explants, respectively. Moreover, these explants produced sevenfold
the number of shoots as similar explants from non-etiolated shoots. All first internodes from etiolated shoots exhibited GUS-expressing
zones and yielded fourfold as many GUS-expressing zones as commonly used leaf explants from non-etiolated shoots, which exhibited
GUS-expressing zones in only 63% of the explants. An average of 9.8 GUS expressing calli per explant were observed on first
internodes from etiolated shoots 2 weeks after cocultivation with A. tumefaciens.
Received: 17 February 1998 / Revision received: 5 May 1998 / Accepted: 15 May 1998 相似文献