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Previous work from this laboratory has shown that isolated chick renal proximal tubule cells possess an Na+-dependent Pi transport system and that Pi uptake is stimulated under gluconeogenic conditions. It is shown in the present paper that gluconeogenesis is associated with a rapid incorporation of Pi into membrane phospholipids, particularly phosphatidylinositol, and some evidence has been obtained for a change in the relative amounts of phosphatidylinositol polyphosphates under gluconeogenic conditions. There is no increase in the total phospholipid phosphate content however, suggesting that pyruvate-induced incorporation of Pi into phospholipids represents accelerated turnover rather than a net increase in synthesis. It is suggested that the stimulation of Na+-dependent Pi uptake by pyruvate is related to the increased rate of phospholipid turnover. Thus Pi transport may be a further example of a physiological system that is influenced by phosphatidylinositol metabolism. The role of phosphatidylinositol phosphates could be to stimulate transfer of transporter molecules from internal stores to the brush-border membrane of the cell. 相似文献
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Isolation,characterization and effect of acidic pH on the unfolding-refolding mechanism of serum albumin domains 总被引:1,自引:0,他引:1
Three fragments,viz., BSA-CNBr1–183, BSA-CNBr184–582, and BSA-T377-582 representing domains I, II + III and III of bovine serum albumin have been isolated and purified. The physicochemical properties
have been investigated and compared with their parent albumin molecule. The values of Stokes radii (nm) and intrinsic viscosities
(ml/g) have been determined to be 2.36, 3.30; 3.43, 4.36; and 2.40, 3.13 for the fragments BSA-CNBr1-183 BSA-CNBr184-582 and BSA-T377-582 respectively. The acid induced unfolding-refolding transitions of intact albumin and the fragment BSA-T377-582 have been shown to occur in two steps while the fragments BSA-CNBr1-183 and BSA-CNBr184-582 underwent single step transitions. The formation of the acid denatured states of intact albumin, BSA-CNBr1–183 and BSA-CNBr184-582 was accompanied by an increase of about 86, 56 and 44% in the values of intrinsic viscosities respectively. Since all the
transitions were reversible, the values of equilibrium constants,K
D, were calculated. The analysis of the dependence ofK
D on pH indicated that the first transition (N-X) of albumin was caused due to the uptake of about 3 protons by the native albumin. The intermediate state,X, is converted to acid unfolded state,D, by taking up another two protons. A comparision of the results on intact albumin with that of its fragments revealed that
the second transition of the fragment BSA-T377–582 and the two single step transitions of the fragment BSA-CNBr1-183 and BSA-CNBr184-582 were much closer to the second transition (X-D) of the intact albumin. The first transition of albumin has been attributed to its domain III represented by the fragment
BSA-T377-582. 相似文献