排序方式: 共有69条查询结果,搜索用时 15 毫秒
21.
Kurtis D. Davies Sakshi Mahale David P. Astling Dara L. Aisner Anh T. Le Trista K. Hinz Aria Vaishnavi Paul A. Bunn Jr Lynn E. Heasley Aik-Choon Tan D. Ross Camidge Marileila Varella-Garcia Robert C. Doebele 《PloS one》2013,8(12)
The targeting of oncogenic ‘driver’ kinases with small molecule inhibitors has proven to be a highly effective therapeutic strategy in selected non-small cell lung cancer (NSCLC) patients. However, acquired resistance to targeted therapies invariably arises and is a major limitation to patient care. ROS1 fusion proteins are a recently described class of oncogenic driver, and NSCLC patients that express these fusions generally respond well to ROS1-targeted therapy. In this study, we sought to determine mechanisms of acquired resistance to ROS1 inhibition. To accomplish this, we analyzed tumor samples from a patient who initially responded to the ROS1 inhibitor crizotinib but eventually developed acquired resistance. In addition, we generated a ROS1 inhibition-resistant derivative of the initially sensitive NSCLC cell line HCC78. Previously described mechanisms of acquired resistance to tyrosine kinase inhibitors including target kinase-domain mutation, target copy number gain, epithelial-mesenchymal transition, and conversion to small cell lung cancer histology were found to not underlie resistance in the patient sample or resistant cell line. However, we did observe a switch in the control of growth and survival signaling pathways from ROS1 to EGFR in the resistant cell line. As a result of this switch, ROS1 inhibition-resistant HCC78 cells became sensitive to EGFR inhibition, an effect that was enhanced by co-treatment with a ROS1 inhibitor. Our results suggest that co-inhibition of ROS1 and EGFR may be an effective strategy to combat resistance to targeted therapy in some ROS1 fusion-positive NSCLC patients. 相似文献
22.
Gupta P Bhatia S Dhawan A Balwani S Sharma S Brahma R Singh R Ghosh B Parmar VS Prasad AK 《Bioorganic & medicinal chemistry》2011,19(7):2263-2268
Regio- and enantioselective synthesis of (S)-(+)-3-arylamino-1-chloropropan-2-ols has been achieved by the epoxide ring opening of (±)-epichlorohydrin with different aromatic amines in the presence of Candida rugosa lipase. Activities of seven model (S)-(+)-3-arylamino-1-chloropropan-2-ols, out of 10 compounds synthesized, have been evaluated for the inhibition of tumor necrosis factor-α TNF-α) induced expression of intercellular adhesion molecule-1 (ICAM-1), which is one of the factors responsible for the modulation of inflammation in biological systems; (S)-(+)-1-chloro-3-(2'-chlorophenylamino)-propan-2-ol has been found to exhibit highest activity, that is, 86% inhibition of TNF-α induced expression of ICAM-1 at a concentration of 40 μg/ml. 相似文献
23.
Rajarajan K Sakshi S Taria S Prathima PT Radhakrishna A Anuragi H Ashajyothi M Bharati A Handa AK Arunachalam A 《Molecular biology reports》2022,49(10):9453-9463
Molecular Biology Reports - Pongamia is considered an important biofuel species worldwide. Drought stress in the early growth stages of Pongamia influences negatively on the germination and... 相似文献
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Rohit Surana Sakshi Sikka Wanpei Cai Eun Myoung Shin Sudha R. Warrier Hong Jie Gabriel Tan Frank Arfuso Simon A. Fox Arun M. Dharmarajan Alan Prem Kumar 《生物化学与生物物理学报:癌评论》2014
The Wnt (wingless-type) signaling pathway plays an important role in embryonic development, tissue homeostasis, and tumor progression becaluse of its effect on cell proliferation, migration, and differentiation. Secreted frizzled-related proteins (SFRPs) are extracellular inhibitors of Wnt signaling that act by binding directly to Wnt ligands or to Frizzled receptors. In recent years, aberrant expression of SFRPs has been reported to be associated with numerous cancers. As gene expression of SFRP members is often lost through promoter hypermethylation, inhibition of methylation through the use of epigenetic modifying agents could renew the expression of SFRP members and further antagonize deleterious Wnt signaling. Several reports have described epigenetic silencing of these Wnt signaling antagonists in various human cancers, suggesting their possible role as tumor suppressors. SFRP family members thus come across as potential tools in combating Wnt-driven tumorigenesis. However, little is known about SFRP family members and their role in different cancers. This review comprehensively covers all the available information on the role of SFRP molecules in various human cancers. 相似文献
26.
Amit Kalra Cameron Roessner Jennifer Jupp Tyler Williamson Raymond Tellier Ahsan Chaudhry Faisal Khan Minakshi Taparia Victor H. Jimenez-Zepeda Douglas A. Stewart Andrew Daly Jan Storek 《Cytotherapy》2018,20(5):706-714
Background
Post-transplant lymphoproliferative disorder (PTLD) is a potentially fatal complication of allogeneic hematopoietic cell transplantation (HCT). Epstein-Barr virus (EBV) reactivation (detectable DNAemia) predisposes to the development of PTLD.Methods
We retrospectively studied 306 patients monitored for EBV DNAemia after Thymoglobulin-conditioned HCT to determine the utility of the monitoring in the management of PTLD. DNAemia was monitored weekly for ≥12 weeks post-transplantation.Results
Reactivation was detected in 82% of patients. PTLD occurred in 14% of the total patients (17% of patients with reactivation). PTLD was treated with rituximab only when and if the diagnosis was established. This allowed us to evaluate potential DNAemia thresholds for pre-emptive therapy. We suggest 100,000–500,000?IU per mL whole blood as this would result in unnecessary rituximab administration to only 4–20% of patients and near zero mortality due to PTLD. After starting rituximab (for diagnosed PTLD), sustained regression of PTLD occurred in 25/25 (100%) patients in whom DNAemia became undetectable. PTLD progressed or relapsed in 12/17 (71%) patients in whom DNAemia was persistently detectable.Discussion
In conclusion, for pre-emptive therapy of PTLD, we suggest threshold DNAemia of 100,000–500,000?IU/mL. Persistently detectable DNAemia after PTLD treatment with rituximab appears to have 71% positive predictive value and 100% negative predictive value for PTLD progression/relapse. 相似文献27.
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Sakshi Shrivastava Ch. V. Siva Kumar Reddy Sharmila S. Mande 《Journal of biosciences》2010,35(3):351-364
Genomic islands (GIs) are regions in the genome which are believed to have been acquired via horizontal gene transfer events
and are thus likely to be compositionally distinct from the rest of the genome. Majority of the genes located in a GI encode
a particular function. Depending on the genes they encode, GIs can be classified into various categories, such as ‘metabolic
islands’, ‘symbiotic islands’, ‘resistance islands’, ‘pathogenicity islands’, etc. The computational process for GI detection
is known and many algorithms for the same are available. We present a new method termed as Improved N-mer based Detection
of Genomic Islands Using Sequence-clustering (INDeGenIUS) for the identification of GIs. This method was applied to 400 completely
sequenced species belonging to proteobacteria. Based on the genes encoded in the identified GIs, the GIs were grouped into
6 categories: metabolic islands, symbiotic islands, resistance islands, secretion islands, pathogenicity islands and motility
islands. Several new islands of interest which had previously been missed out by earlier algorithms were picked up as GIs
by INDeGenIUS. The present algorithm has potential application in the identification of functionally relevant GIs in the large
number of genomes that are being sequenced. Investigation of the predicted GIs in pathogens may lead to identification of
potential drug/vaccine candidates. 相似文献
29.
Mark Turlington Aspen Chun Sakshi Tomar Aimee Eggler Valerie Grum-Tokars Jon Jacobs J. Scott Daniels Eric Dawson Adrian Saldanha Peter Chase Yahira M. Baez-Santos Craig W. Lindsley Peter Hodder Andrew D. Mesecar Shaun R. Stauffer 《Bioorganic & medicinal chemistry letters》2013,23(22):6172-6177
Herein we report the discovery and SAR of a novel series of SARS-CoV 3CLpro inhibitors identified through the NIH Molecular Libraries Probe Production Centers Network (MLPCN). In addition to ML188, ML300 represents the second probe declared for 3CLpro from this collaborative effort. The X-ray structure of SARS-CoV 3CLpro bound with a ML300 analog highlights a unique induced-fit reorganization of the S2–S4 binding pockets leading to the first sub-micromolar noncovalent 3CLpro inhibitors retaining a single amide bond. 相似文献
30.
Expression of KdpFABC, a K+ pump that restores osmotic balance, is controlled by binding of the response regulator KdpE to a specific DNA sequence (kdpFABCBS) via the winged helix-turn-helix type DNA binding domain (KdpEDBD). Exploration of E. coli KdpEDBD and kdpFABCBS interaction resulted in the identification of two conserved, AT-rich 6 bp direct repeats that form half-sites. Despite binding to these half-sites, KdpEDBD was incapable of promoting gene expression in vivo. Structure-function studies guided by our 2.5 Å X-ray structure of KdpEDBD revealed the importance of residues R193 and R200 in the α-8 DNA recognition helix and T215 in the wing region for DNA binding. Mutation of these residues renders KdpE incapable of inducing expression of the kdpFABC operon. Detailed biophysical analysis of interactions using analytical ultracentrifugation revealed a 2∶1 stoichiometry of protein to DNA with dissociation constants of 200±100 and 350±100 nM at half-sites. Inactivation of one half-site does not influence binding at the other, indicating that KdpEDBD binds independently to the half-sites with approximately equal affinity and no discernable cooperativity. To our knowledge, these data are the first to describe in quantitative terms the binding at half-sites under equilibrium conditions for a member of the ubiquitous OmpR/PhoB family of proteins. 相似文献