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排序方式: 共有452条查询结果,搜索用时 15 毫秒
101.
Igawa Tatsuhiro Kishikawa Shuhei Abe Yoshito Yamashita Tomohiro Nagai Saki Shiroishi Mitsunori Shinozaki Chinatsu Tanaka Hiroyuki Tozaki-Saitoh Hidetoshi Tsuda Makoto Inoue Kazuhide Ueda Tadashi 《Purinergic signalling》2019,15(1):27-35
Purinergic Signalling - P2X purinergic receptors are ATP-driven ionic channels expressed as trimers and showing various functions. A subtype, the P2X4 receptor present on microglial cells is highly... 相似文献
102.
Kazuhito Fujiyama Hideaki Maki Shinichi Kinoshita Toshiomi Yoshida 《FEMS microbiology letters》1995,126(1):19-23
Abstract The plasmid pAL205 encodes an alginate lyase gene of Pseudomonas sp. OS-ALG-9, fused in frame to the β-galactosidase α-peptide gene. The alginate lyase (Aly) expressed in Escherichia coli (pAL205) was significantly secreted into the medium by the addition of glycine. The extracellular enzyme isolated from the culture of E. coli JM109 (pAL205) was purified over 15 000-fold by successive chromatography and subjected to amino acid sequence analysis. The sequence determined was identical to that of the intracellular protein. Since the activity and molecular size of the extracellular Aly is identical to the intracellular protein and to the Aly isolated from Pseudomonas , the glycine does not affect or modify the Aly during its leakage into the medium. 相似文献
103.
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105.
Individual variation in two species of host plants (thistle,Cirsium kamtschaticum, and blue cohosh,Caulophyllum robustum) of the herbivorous ladybird beetleEpilachna pustulosa was examined under laboratory conditions for their acceptability to adult beetles as a food resource, for adult preference
and for larval performance. When clones of these plants were subjected to non-choice tests using posthibernating female beetles,
there was found to be significant intraspecific variation among clones in terms of their acceptability, but interspecific
variation was not detected. Significant intraspecific as well as interspecific variation were frequently detected in the two
host plants when clones of these plants were subjected to choice tests using posthibernating female beetles; the magnitude
of interspecific plant variation for beetle preference is not necessarily larger than that of intraspecific plant variation.
Individual variation across plant species with respect to beetle larval performance was also significant. A positive correlation
between adult preference and larval performance is suggested across the two taxonomically remote host plant species, thistle
and blue cohosh, although this needs further investigation. 相似文献
106.
Moran D.G. Yoshida S. Fujiyama K. Seki T. Yoshida T. 《World journal of microbiology & biotechnology》1997,13(3):265-267
A truncated Man9-mannosidase gene and a full-length -1,4-galactosyltransferase gene were isolated from human kidney and placenta cDNAs, respectively. Both genes were cloned in plasmid pMAL-c2 to produce fusions with maltose-binding protein. Fusion products were purified by affinity chromatography. Purified enzymes were assayed using pyridyl-amino labelled oligosaccharides as substrates and analysed by HPLC. 相似文献
107.
Saki Shimamoto Mana Kawaguchi Kazuki Nakashima Akira Ohtsuka 《Bioscience, biotechnology, and biochemistry》2016,80(7):1382-1385
The gene expression pattern of the glucose transporters (GLUT1, GLUT3, GLUT8, and GLUT12) among pectoralis major and minor, biceps femoris, and sartorius muscles from newly hatched chicks was examined. GLUT1 mRNA level was higher in pectoralis major muscle than in the other muscles. Phosphorylated AKT level was also high in the same muscle, suggesting a relationship between AKT and GLUT1 expression. 相似文献
108.
Takeshi Takarada Masato Ogura Noritaka Nakamichi Takami Kakuda Ryota Nakazato Hiroshi Kokubo Shinsuke Ikeno Saki Nakamura Takaya Kutsukake Eiichi Hinoi Yukio Yoneda 《Neurochemical research》2016,41(1-2):5-15
We have shown marked promotion of both cluster growth and neuronal specification in pluripotent P19 cells with overexpression of solute carrier 38a1 (Slc38a1), which is responsible for membrane transport of glutamine. In this study, we evaluated pharmacological profiles of the green tea amino acid ingredient theanine, which is a good substrate for glutamine transporters, on proliferation and neuronal specification in neural progenitor cells from embryonic rat neocortex. Sustained exposure to theanine, but not glutamine, accelerated the growth of neurospheres composed of proliferating cells and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reducing activity at concentrations of 1–100 μM in undifferentiated progenitor cells. Such prior exposure to theanine promoted spontaneous and induced commitment to a neuronal lineage with concomitant deteriorated astroglial specification. Selective upregulation was seen in the expression of Slc38a1 in progenitor cells cultured with theanine. Similarly significant increases in cluster growth and MTT reducing activity were found in P19 cells cultured with theanine for 4 days. Luciferase activity was doubled in a manner sensitive to the deletion of promoter regions in P19 cells with a luciferase reporter plasmid of the Slc38a1 promoter after sustained exposure to theanine for 4 days. Overexpression of X-box binding protein-1 led to a marked increase in luciferase activity in P19 cells transfected with the Slc38a1 reporter plasmid. These results suggest that theanine accelerates cellular proliferation and subsequent neuronal specification through a mechanism relevant to upregulation of Slc38a1 gene in undifferentiated neural progenitor cells. 相似文献
109.
110.
Hitoshi Nakashima Asao Fujiyama Shunjiro Kagiyama Takashi Imamura 《Human genetics》1990,84(6):568-570
Summary Of the 645 Japanese subjects studied, we have identified 10 individuals heterozygous for a chromosome with the triplicated -globin loci. The frequency of the triple -loci was 0.008 in this population, while that of the single -locus, i.e., -thalassemia 2 gene, might be lower than 0.0008. Analysis of haplotypes using particular RsaI site polymorphism in the -globin gene complex strongly suggests that the triple loci may have had multiple origins in this population. 相似文献