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31.
Arias N Macarulla MT Aguirre L Martínez-Castaño MG Gómez-Zorita S Miranda J Martínez JA Portillo MP 《Journal of physiology and biochemistry》2011,67(3):471-477
Scientific research is constantly looking for new molecules to be used as functional ingredients to combat obesity. The aim
of the present study was to analyse whether resveratrol and conjugated linoleic acid (CLA) together could reduce body fat
more efficiently than their separate administration. Thirty-six male Wistar rats were randomly divided into four groups: controls
rats (C), rats treated with resveratrol (RSV), rats treated with CLA (CLA) and rats treated with a combination of resveratrol
and CLA (RSV+CLA). All rats were fed on an obesogenic diet. In RSV and RSV+CLA groups, the rats received 30 mg resveratrol/kg
body weight/day. In CLA and RSV+CLA groups, an equimolecular mixture of trans-10,cis-12 and cis-9,trans-11 was added to the diet to reach 0.5% of the active isomer trans-10,cis-12. After 6 weeks of treatment, white adipose tissue from different anatomical locations was dissected and weighed. Serum
triacylglycerols, total and HDL cholesterols, glucose, insulin, fructosamine and TNF-α were measured. A glucose tolerance
test was also performed. Separately, resveratrol and CLA significantly reduced body fat but did not do so when combined: 20%
in the RSV group and 18% in CLA group but 7% in the RSV+CLA group. Resveratrol reduced serum triacylglycerols. No differences
were found among groups in serum cholesterol. Resveratrol, as well as the combination RSV+CLA, improved glycaemic control.
These results demonstrate that the combination RSV+CLA reduces the effectiveness of each compound on body fat-lowering action,
but it maintains the positive effect of resveratrol on glycaemic control. Consequently, this combination has no usefulness
in obesity prevention. 相似文献
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33.
Angiogenic network formation in the developing vertebrate trunk 总被引:12,自引:0,他引:12
Isogai S Lawson ND Torrealday S Horiguchi M Weinstein BM 《Development (Cambridge, England)》2003,130(21):5281-5290
We have used time-lapse multiphoton microscopy of living Tg(fli1:EGFP)y1 zebrafish embryos to examine how a patterned, functional network of angiogenic blood vessels is generated in the early vertebrate trunk. Angiogenic vascular sprouts emerge from the longitudinal trunk axial vessels (the dorsal aorta and posterior cardinal vein) in two spatially and temporally distinct steps. Dorsal aorta-derived sprouts form an initial primary network of vascular segments, followed by emergence of vein-derived secondary vascular sprouts that interact and interconnect dynamically with the primary network to initiate vascular flow. Using transgenic silent heart mutant embryos, we show that the gross anatomical patterning of this network of vessels does not require blood circulation. However, our results suggest that circulatory flow dynamics play an important role in helping to determine the pattern of interconnections between the primary network and secondary sprouts, and thus the final arterial or venous identity of the vessels in the functional network. We discuss a model to explain our results combining genetic programming of overall vascular architecture with hemodynamic determination of circulatory flow patterns. 相似文献
34.
Fiona Gordon Saioa Elcoroaristizabal Alan G. Ryder 《Biochimica et Biophysica Acta (BBA)/General Subjects》2021,1865(2):129770
BackgroundFörster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. Accurate FRET analysis, in terms of measuring changes in donor and acceptor spectra and energy transfer efficiency is therefore critical.MethodsWe demonstrate a novel quantitative FRET analysis using anisotropy resolved multidimensional emission spectroscopy (ARMES) in a Human Serum Albumin (HSA) and 1,8-anilinonaphathalene sulfonate (ANS) model. ARMES combines 4D measurement of polarized excitation emission matrices (pEEM) with multivariate data analysis to spectrally resolve contributing fluorophores. Multivariate analysis (Parallel Factor, PARAFAC and restricted Tucker3) was used to resolve fluorophore contributions and for modelling the quenching of HSA emission and the HSA-ANS interactions.ResultspEEM spectra were modelled using Tucker3 which accommodates non-linearities introduced by FRET and a priori chemical knowledge was used to optimise the solution, thus resolving three components: HSA emission, ANS emission from indirect FRET excitation, and ANS emission from direct excitation. Perpendicular emission measurements were more sensitive to indirectly excited acceptor emission. PARAFAC modelling of HSA, donor emission, separated ANS FRET interacting (Tryptophan) and non-interacting (Tyrosine) components. This enabled a new way of calculating quenching constants using the multi-dimensional emission of individual donor fluorophores.ConclusionsFRET efficiency could be calculated using the multi-dimensional, resolved emission of the interacting donor fluorophores only which yielded higher ET efficiencies compared to conventional methods.General significanceShows the potential of multidimensional fluorescence measurements and data analysis for more accurate FRET modelling in proteins. 相似文献
35.
Gómez-Zorita Saioa Queralt Maite Vicente Maria Angeles González Marcela Portillo María P. 《Journal of physiology and biochemistry》2021,77(1):175-189
Journal of Physiology and Biochemistry - Despite the general relationship between obesity and its co-morbidities, there are both obese individuals who scarcely present the associated pathologies... 相似文献