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141.
142.
Saifun Nahar Yoshiki Nakashima Chika Miyagi-Shiohira Takao Kinjo Zensei Toyoda Naoya Kobayashi Issei Saitoh Masami Watanabe Hirofumi Noguchi Jiro Fujita 《World journal of stem cells》2018,10(11):146-159
Adipose-derived mesenchymal stem cells(ADSCs) are a treatment cell source for patients with chronic liver injury. ADSCs are characterized by being harvested from the patient's own subcutaneous adipose tissue, a high cell yield(i.e., reduced immune rejection response), accumulation at a disease nidus, suppression of excessive immune response, production of various growth factors and cytokines, angiogenic effects, antiapoptotic effects, and control of immune cells via cellcell interaction. We previously showed that conditioned medium of ADSCs promoted hepatocyte proliferation and improved the liver function in a mouse model of acute liver failure. Furthermore, as found by many other groups, the administration of ADSCs improved liver tissue fibrosis in a mouse model of liver cirrhosis. A comprehensive protein expression analysis by liquid chromatography with tandem mass spectrometry showed that the various cytokines and chemokines produced by ADSCs promote the healing of liver disease. In this review, we examine the ability of expressed protein components of ADSCs to promote healing in cell therapy for liver disease. Previous studies demonstrated that ADSCs are a treatment cell source for patients with chronic liver injury. This review describes the various cytokines and chemokines produced by ADSCs that promote the healing of liver disease. 相似文献
143.
Introduction of functional groups onto polypropylene and polyethylene surfaces for immobilization of enzymes 总被引:3,自引:0,他引:3
Polypropylene and polyethylene surfaces are activated by introducing an active functional group through 1-fluoro-2 nitro-4-azidobenzene by UV irradiation. Horseradish peroxidase and glucose oxidase are immobilized onto the activated surfaces, simply by incubating the enzymes at 37 degrees C. When untreated surfaces are used, insignificant immobilization of the enzymes is observed. 相似文献
144.
Caren Weinhouse Olivia S Anderson Tamara R Jones Jung Kim Shayna A Liberman Muna S Nahar Laura S Rozek Randy L Jirtle Dana C Dolinoy 《Epigenetics》2011,6(9):1105-1113
Genetic loci displaying environmentally responsive epigenetic marks, termed metastable epialleles, offer a solution to the paradox presented by genetically identical yet phenotypically distinct individuals. The murine viable yellow agouti (Avy) metastable epiallele exhibits stochastic DNA methylation and histone modifications associated with coat color variation in isogenic individuals. The distribution of Avy variable expressivity shifts following maternal nutritional and environmental exposures. To characterize additional murine metastable epialleles, we utilized genome-wide expression arrays (N = 10 male individuals, 3 tissues per individual) and identified candidates displaying large variability in gene expression among individuals (Vi = inter-individual variance), concomitant with a low variability in gene expression across tissues from the three germ layers (Vt = inter-tissue variance), two features characteristic of the Avy metastable epiallele. The CpG island in the promoter of Dnajb1 and two contraoriented ERV class II repeats in Glcci1 were validated to display underlying stochasticity in methylation patterns common to metastable epialleles. Furthermore, liver DNA methylation in mice exposed in utero to 50 mg bisphenol A (BPA)/kg diet (N = 91) or a control diet (N = 79) confirmed environmental lability at validated candidate genes. Significant effects of exposure on mean CpG methylation were observed at the Glcci1 Repeat 1 locus (p < 0.0001). Significant effects of BPA also were observed at the first and fifth CpG sites studied in Glcci1 Repeat 2 (p < 0.0001 and p = 0.004, respectively). BPA did not affect methylation in the promoter of Dnajb1 (p = 0.59). The characterization of metastable epialleles in humans is crucial for the development of novel screening and therapeutic targets for human disease prevention.Key words: epigenetics, metastable epiallele, viable yellow agouti, environmental epigenomics 相似文献
145.
Nucleolar organizer regions are nucleolar components that contain proteins that are stained selectively by silver methods; they can be identified as black dots throughout the nucleolus and are known as silver binding nucleolar organizer regions (AgNOR). The number of AgNOR is related to the cell cycle and the proliferative activity of the cells. We investigated AgNOR using exfoliative cytology smears of potentially malignant oral lesions. Eighty individuals were divided into four equal groups: healthy controls, oral leukoplakia, oral submucous fibrosis and oral squamous cell carcinoma. The mean number of AgNOR in each study group gradually increased from control to oral leukoplakia to oral submucous fibrosis to oral squamous cell carcinoma. The proliferative index was increased in the oral premalignant and malignant patients compared to normal subjects. The mean AgNOR size gradually increased from control to oral leukoplakia to oral submucous fibrosis to oral squamous cell carcinoma. Spherical shaped AgNOR were most common in controls, whereas large, clustered and kidney shapes were most common in oral squamous cell carcinoma. Multiparameter analysis of AgNOR in oral exfoliative smears is a simple, sensitive and cost-effective method for differentiating premalignant from malignant lesions and can be used in conjunction with routine cytomorphological evaluation. 相似文献
146.
Zabun Nahar Mohammad Abul Kalam Azad Md Ashrafur Rahman Mohammad Arifur Rahman Wasimul Bari Sheikh Nazrul Islam Md Saiful Islam Abul Hasnat 《Biological trace element research》2010,133(3):284-290
The purpose of the study was to determine the serum concentration of trace elements of panic disorder patients and to find
out the relationship between trace element levels and nutritional status or socio-economic factors. The study was conducted
among 54 panic disorder patients and 52 healthy volunteers. Patients were recruited from Bangabandhu Sheikh Mujib Medical
University by random sampling. Serum trace element concentrations were determined by flame atomic absorption spectroscopy
(for Mg, Zn, Ca, and Cu) as well as graphite furnace (for Mn). Data were analyzed by independent t test, Pearson’s correlation analysis, regression analysis, and ANOVA. The serum concentration of Mn, Zn, Ca, Cu, and Mg in
panic disorder patients were 0.37 ± 0.30, 0.67 ± 0.20, 99.91 ± 15.15, 0.83 ± 0.23, and 21.14 ± 3.72 mg/L, while those were
0.4163 ± 0.2527, 0.86 ± 0.3, 106.6073 ± 18.6531, 0.8514 ± 0.3646, and 21.37 ± 2.03 mg/L in control subjects, respectively.
The serum concentration of Zn decreased significantly (p = 0.001) in patient group. But the differences of the concentration of Mn, Ca, Cu, and Mg between patient and control group
were not significant (p = 0.522, p = 0.065, p = 0.800, and p = 0.712, respectively). Socio-economic data reveal that most of the patients were very poor and middle aged. Mean BMIs of
the control group (23.74 ± 2.71 kg/m2) and the patient group (22.62 ± 3.74 kg/m2) were within the normal range (18.5–25.0 kg/m2). There was no significant relationship between serum zinc level and BMI of patients (r = 0.038; p = 0.809). So the decreased level of serum zinc in panic disorder patients was not because of other reasons, but rather it
may provide a prognostic tool for the diagnosis and treatment of this disease. 相似文献
147.
Shamim Ahmad Parminder Singh Otaal Taranjit Singh Rai Ajay Bahl Uma Nahar Saikia Rohit K. Manoj M. Thungapathra Kewal K. Talwar Madhu Khullar 《Molecular and cellular biochemistry》2009,324(1-2):139-145
Background Increased levels of TNF-α, IL-6, their soluble receptors, and NT-proBNP have been observed in patients with dilated cardiomyopathy (DCM). In the present study, we assessed the possible involvement of proinflammatory cytokines and their soluble receptors with and without recovery of LV function in DCM patients. Methods and results Forty patients with DCM were enrolled and divided into two groups: Group I consisted of DCM patients (n = 30) whose left ventricular ejection fraction (LVEF) had not recovered on follow up and Group II comprised DCM patients (n = 10) whose LVEF had recovered. Ten healthy subjects were included as controls (Group III). TNF-α, IL-6,TNFR1, TNFRII, gp130, and NT-proBNP levels were significantly increased in Group I and were significantly lower in patients with LVEF recovery as compared to those without recovery of LVEF (P < 0.05). Conclusion Circulating TNF-α, IL-6, and NT-proBNP appear to correlate with the LV function recovery of patients with DCM and could be used as prognostic biomarkers. 相似文献
148.
149.
BACKGROUND: The mediastinum is an uncommon site for liposarcoma, with <1 % of all tumors occurring in this site. CASE: A 40-year-old woman presented with superior vena caval syndrome. Radiologic investigations revealed the presence of a large soft tissue mass occupying the anterior and middle mediastinum. A computed tomography (CT)-guided fine needle aspiration cytology (FNAC) sample showed the presence of fibrillary myxoid material with arborizing blood vessels and atypical lipoblasts. A diagnosis of myxold liposarcoma was made, which was later confirmed on bistopathology. CONCLUSION: The mediastinum is a challenging area for FNAC, which is a useful tool for accurate diagnosis. Awareness of the presence of liposarcoma is important for its recognition. 相似文献
150.
Abdul Awal Chowdhury Masud Md. Fazlul Karim M. H. M. Borhannuddin Bhuyan Jubayer Al Mahmud Kamrun Nahar Masayuki Fujita Mirza Hasanuzzaman 《Phyton》2021,90(2):353-372
Drought is the most common form of abiotic stress that reduces plant growth and productivity. It causes plant
injuries through elevated production of reactive oxygen species (ROS). Potassium (K) is a vital plant nutrient that
notably ameliorates the detrimental effect of drought stress in the plant. A pot experiment was conducted at the
Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Japan, under controlled environment of green house to explore the role of K in mitigating drought severity in wheat (Triticum asevitum L.)
seedlings. Three days after germination, seedlings were exposed to three water regimes viz., 100, 50, and 20% field
capacity (FC) for 21 days. Potassium was adjusted in Hoagland nutrient solution at 0, 6 and 12 mM concentration
and applied to pot instead of normal water. Results show that, water deficit stress notably reduced plant growth,
biomass accumulation, leaf relative water content (RWC) along with reduced photosynthetic pigments. Increased
amount of biochemical stress markers viz., malondialdehyde (MDA), hydrogen peroxide (H2O2), methylglyoxal
(MG), proline (Pro) as well as an impaired antioxidant defense system were observed in drought affected wheat
plants. On the contrary, K supplementation resulted in improvement of biochemical and physiological parameters
that worked behind in improving growth and development of the wheat plants. In addition, enzymes of ascorbateglutathione (AsA-GSH) cycle were also enhanced by supplemented K that accelerated the ROS detoxification process in plant. Although glyoxalse system did not performed well till MG was detoxified might following another
short stepped pathways. Our results revealed that drought stressed plants showed better performances in terms of
biochemical and physiological attributes, antioxidant defense and glyoxalase system, as well as ROS detoxification
due to K supplementation with better performance at 12 mM K added in 50% FC growing condition. 相似文献