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Application of Soil‐borne Actinomycetes for Biological Control against Fusarium Wilt of Chickpea (Cicer arietinum) caused by Fusarium solani fsp pisi
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Mahboobeh Soltanzadeh Meysam Soltani Nejad Gholam Hosein Shahidi Bonjar 《Journal of Phytopathology》2016,164(11-12):967-978
Black root rot, caused by Fusarium solani f.sp. pisi, is a devastating soil‐borne disease in chickpea in Iran with no effective control measures. With the aim of finding applicable biocontrol agents to alleviate the malady, isolates of Actinomycetes isolated from soil and their antagonistic effect against F. solani f.sp. pisi were evaluated both in vitro and in vivo. More than 100 Actinomycetes isolates were screened for their antifungal activities against the pathogen. The most active isolates were evaluated in greenhouse for their biocontrol performance. Based on the results of dual cultures in screening evaluations, the size of inhibition zone of fungal growth, and the most effective antagonist isolates (S3, S12 and S40) were selected for further studies. Identity of active isolates was determined, in this regard, 16S rDNA of isolates were amplified using universal bacterial primers FD1 and RP2. The PCR products were purified and sequenced. Sequence analysis of 16S rDNA was then performed using NCBI BLAST method. Comparison of the near full length 16S rRNA sequence of isolates to GenBank sequences demonstrated that isolates S3 and S12 were most similar to Streptomyces antibioticus, while isolate S40 was most similar to Streptomyces peruviensis. Biocontrol studies of these isolates in control of the disease in greenhouse significantly decreased the disease severity. Actinomycetes isolate S12 demonstrated the greatest effect in reducing disease than the other two. Results of this research are at preliminary stage for developing biocontrol agents. These data can be utilized as a platform for future studies with the aim of commercializing these biocontrol products and hoping to step towards sustainable agriculture. 相似文献
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Danya Abazari Mehrad Moghtadaei Ali Behvarmanesh Bahareh Ghannadi Monireh Aghaei Mahboobeh Behruznia Garshasb Rigi 《Bioinformation》2015,11(5):243-247
Ebola virus is a member of Filoviridae and cause severe human disease with 90 percent mortality. The life cycle of Ebola contains
an assembly stage which is mediated by VP40 proteins. VP40 subunits oligomerize and form ring-structures which are either
octamers or hexamers. Prevention of VP40 matrix protein assembly prevents virus particle formation as well as virus budding. In
the present study we simulated the biological condition for a single VP40 subunit. Then a library containing 120.000 drugs like
chemicals was used as the virtual screening database. Top 10 successive hits were then analyzed regarding absorption, distribution,
metabolism, and excretion properties. Moreover probable accessorial human protein targets and toxicity properties of successive
hits were analyzed by in silico tools. We found 4 chemicals that could bind VP40 subunits in a manner that by making an
interfering steric condense prevents matrix protein oligomerization. The pharmacokinetic and toxicity studies also validated the
potential of 4 finlay successive hits to be considered as a new anti-Ebola drugs. 相似文献
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G. Mustafa Saifi Reiner Veitia Houssein Khodjet El Khil Sandrine Barbaux Preetha Tilak I. Manorama Thomas Marc Fellous 《Journal of genetics》2000,79(1):17-20
During a study of deletions of Y-chromosomal DNA in infertile males, sY116, a Y-linked STS, showed different electrophoretic
mobilities in three males, two infertile and one fertile. A study of this STS among 35 other normal males showed that this
locus is polymorphic. sY1 16 has a polyA-rich stretch whose instability appears to be the most likely cause of this polymorphism.
The possible usefulness of sY116 polymorphism in the detection of subtle genome-wide instabilities in some types of cancer
is discussed. 相似文献
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In the present studies we examined the effects of an intra-peritoneal injection of morphine (7 mg/kg) on carrageenan-induced paw oedema in mice. Carrageenan-induced paw oedema was measured by mercury plethysmometer and was maximal at hour 3, and pretreatment with morphine could reduce the oedema significantly. At the same time the serum levels of interleukin-1 alpha (IL-1 alpha) were increased. Pretreatment with naloxone and dexamethasone abolished morphine anti-inflammatory while decreasing IL-1 alpha serum levels, significantly. These findings suggest that an increase in serum levels of IL-1 alpha plays an important role in the anti-inflammatory effect of morphine. 相似文献
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Sensitive determination of clarithromycin in human plasma by high-performance liquid chromatography with spectrophotometric detection 总被引:2,自引:0,他引:2
Amini H Ahmadiani A 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2005,817(2):193-197
A rapid, selective and sensitive high-performance liquid chromatographic method with spectrophotometric detection was developed for the determination of clarithromycin in human plasma. Liquid-liquid extraction of clarithromycin and norverapamil (as internal standard) from plasma samples was performed with n-hexane/1-butanol (98:2, v/v) in alkaline condition followed by back-extraction into diluted acetic acid. Chromatography was carried out using a CN column (250 mm x 4.6 mm, 5 microm) under isocratic elution with acetonitrile-50 mM aqueous sodium dihydrogen phosphate (32:68, v/v), pH 4.5. Detection was made at 205 nm and analyses were run at a flow-rate of 1.0 ml/min at 40 degrees C. The analysis time was less than 11 min. The method was specific and sensitive with a quantification limit of 31.25 ng/ml and a detection limit of 10 ng/ml in plasma. The mean absolute recovery of clarithromycin from plasma was 95.9%, while the intra- and inter-day coefficient of variation and percent error values of the assay method were all less than 9.5%. Linearity was assessed in the range of 31.25-2000 ng/ml in plasma with a correlation coefficient of greater than 0.999. The method was used to analyze several hundred human plasma samples for bioavailability studies. 相似文献
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Rapid determination of loratadine in small volume plasma samples by high-performance liquid chromatography with fluorescence detection 总被引:2,自引:0,他引:2
Amini H Ahmadiani A 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,809(2):227-230
A simple and rapid high-performance liquid chromatographic method with fluorescence detection was developed for the determination of loratadine in small volume plasma samples. Liquid-liquid extraction of loratadine and diazepam (as internal standard) from plasma samples was performed with n-butyl alcohol/n-hexane (2:98, v/v) in alkaline condition followed by back-extraction into diluted perchloric acid. Chromatography was carried out using a C8 column (250 x 4.6 mm, 5 microm) under isocratic elution with acetonitrile-20 mM sodium dihydrogen phosphate-triethylamine (43:57:0.02, v/v), pH 2.4. Analyses were run at a flow-rate of 1.0 ml/min at room temperature. The method was specific and sensitive with a quantitation limit of 0.62 ng/ml and a detection limit of 0.2 ng/ml at a signal-to-noise ratio of 3:1. The mean absolute recovery of loratadine from plasma was 84%, while the intra-and inter-day coefficient of variation and percent error values of the assay method were all less than 9.7%. Linearity was assessed in the range of 0.62-20 ng/ml in plasma with a correlation coefficient of greater than 0.999. The method has been used to analyze several hundred human plasma samples for bioavailability studies. 相似文献
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Fatemeh Hemmati Rasoul Ghasemi Norlinah Mohamed Ibrahim Leila Dargahi Zahurin Mohamed Azman Ali Raymond Abolhassan Ahmadiani 《Molecular neurobiology》2014,50(3):797-810
Neuroinflammation is known as a key player in a variety of neurodegenerative and/or neurological diseases. Brain Toll-like receptors (TLRs) are leading elements in the initiation and progression of neuroinflammation and the development of different neuronal diseases. Furthermore, TLR activation is one of the most important elements in the induction of insulin resistance in different organs such as the central nervous system. Involvement of insulin signaling dysregulation and insulin resistance are also shown to contribute to the pathology of neurological diseases. Considering the important roles of TLRs in neuroinflammation and central insulin resistance and the effects of these processes in the initiation and progression of neurodegenerative and neurological diseases, here we are going to review current knowledge about the potential crosstalk between TLRs and insulin signaling pathways in neuroinflammatory disorders of the central nervous system. 相似文献