全文获取类型
收费全文 | 580篇 |
免费 | 32篇 |
国内免费 | 1篇 |
出版年
2024年 | 3篇 |
2023年 | 11篇 |
2022年 | 22篇 |
2021年 | 45篇 |
2020年 | 30篇 |
2019年 | 59篇 |
2018年 | 26篇 |
2017年 | 28篇 |
2016年 | 29篇 |
2015年 | 29篇 |
2014年 | 38篇 |
2013年 | 44篇 |
2012年 | 43篇 |
2011年 | 52篇 |
2010年 | 25篇 |
2009年 | 14篇 |
2008年 | 13篇 |
2007年 | 26篇 |
2006年 | 10篇 |
2005年 | 11篇 |
2004年 | 13篇 |
2003年 | 12篇 |
2002年 | 9篇 |
1995年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 6篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1982年 | 2篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有613条查询结果,搜索用时 15 毫秒
601.
Elseweidy Mohamed M. Elswefy Sahar E. Younis Nahla N. Tarek Shaden 《Molecular biology reports》2019,46(4):3921-3928
Molecular Biology Reports - 10-Dehydrogingerdione (10-DHGD) was previously reported to possess a hypolipidemic, anti-inflammatory and anti-oxidant properties in hyperlipidemic rabbit model. In this... 相似文献
602.
603.
604.
Maral Nouri Hajbaba Mehrab Pourmadadi Fatemeh Yazdian Hamid Rashedi Majid Abdouss Dina Sadat Zhohrabi 《Biotechnology progress》2023,39(1):e3305
In recent decades, magnetic nanoparticles modified with biocompatible polymers have been recognized as a suitable tool for treating breast cancer. The aim of this research was to evaluate the function of chitosan/agarose-functionalized Fe2O3 nanoparticles on the MCF-7 breast cancer cell line and the expression of BCL2 and BAX genes. Free Fe2O3 nanoparticles were prepared by hydrothermal method. FTIR, XRD, SEM, DLS, VSM, and zeta potential analyses determined the size and morphological characteristics of the synthesized nanoparticles. The effect of Fe2O3 free nanoparticles and formulated Fe2O3 nanoparticles on induction of apoptosis was studied by double-dye Annexin V-FITC and PI. Also, the gene expression results using the PCR method displayed that Fe2O3 formulated nanoparticles induced BAX apoptosis by increasing the anti-apoptotic gene expression and decreasing the expression of pro-apoptotic gene BCL2, so the cell progresses to planned cell death. In addition, the results showed that the BAX/BCL2 ratio decreased significantly after treatment of MCF-7 cells with free Fe2O3 nanoparticles, and the BAX/BCL2 ratio for Fe2O3 formulated nanoparticles increased significantly. Also, to evaluate cell migration, the scratch test was performed, which showed a decrease in motility of MCF-7 cancer cells treated with Fe2O3 nanoparticles formulated with chitosan/agarose at concentrations of 10, 50, 100, and 200 μg/ml. 相似文献
605.
Rania H. Abd El-Hameed Shahenda Mahgoub Hend M. El-Shanbaky Mosaad S. Mohamed Sahar A. Ali 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):977
Inflammation is associated with the development of several diseases comprising cancer and cardiovascular disease. Agents that suppress cyclooxygenase (COX) and lipoxygenase (LOX) enzymes, besides chemokines have been suggested to minimise inflammation. Here, a variety of novel heterocyclic and non-heterocyclic compounds were prepared from novel three furanone derivatives. The structures of all synthesised compounds were confirmed by elemental and spectral analysis including mass, IR, and 1H-NMR spectroscopy. Anti-inflammatory activities of these synthesised compounds were examined in vitro against COX enzymes, 15-LOX, and tumour necrosis factor-α (TNF-α), using inhibition screening assays. The majority of these derivatives showed significant to high activities, with three pyridazinone derivatives (5b, 8b, and 8c) being the most promising anti-inflammatory agents with dual COX-2/15-LOX inhibition activities along with high TNF-α inhibition activity. 相似文献
606.
Elham Khodaverdi Farnaz Sadat Mirzazadeh Tekie Farzin Hadizadeh Haydar Esmaeel Seyed Ahmad Mohajeri Sayyed A. Sajadi Tabassi Gholamhossein Zohuri 《AAPS PharmSciTech》2014,15(1):177-188
Although conventional pharmaceuticals have many drug dosage forms on the market, the development of new therapeutic molecules and the low efficacy of instant release formulations for the treatment of some chronic diseases and specific conditions encourage scientists to invent different delivery systems. To this purpose, a supramolecular hydrogel consisting of the tri-block copolymer PLGA-PEG-PLGA and α-cyclodextrin was fabricated for the first time and characterised in terms of rheological, morphological, and structural properties. Naltrexone hydrochloride and vitamin B12 were loaded, and their release profiles were determined. 相似文献
607.
Françoise Bernard Azar Hassanpour Gila Gholizadeh Sahar Hassannejad Zahede Chaghari 《Acta Physiologiae Plantarum》2011,33(2):431-436
In this study, the ability of safflower-isolated root cultures to produce yellow pigments was tested. Initially, the growth
of isolated roots in static liquid medium was evaluated with different volumes of culture medium. A volume of 6 ml of medium
per flask of 250 ml gave the best growth performance and, in this condition of culture, production of pigments from isolated
roots treated or not by light has been determined by spectrophotometry (321 and 400 nm). Under these conditions, the production
of yellow pigments amounted to 13.18 mg g−1 fresh weight and the light stimulated the synthesis of these pigments by isolated roots. Total yellow pigments of 24.12,
38.91 and 46.38 mg g−1 fresh weight was produced by the roots treated with 9, 13.5 and 18% (v/v) gas oil, respectively, representing high values
of production. The pigments were released in large quantities in the medium. The increased synthesis of pigments as a result
of gas oil treatment was accompanied by a reduction of the peroxidase activity of roots. Given the high production of yellow
pigments, systems of isolated root culture could be considered for the study of a larger scale production of safflower pigments
widely used for various industrial purposes. 相似文献
608.
Dorafshan Tabatabai Ashraf Sadat Dehghanian Effat Mansouri-Torshizi Hassan 《Biometals》2022,35(2):245-266
BioMetals - Treatment with transition metal complexes is an efficient method to fight with cancer. Therefore, a new transition metal complex formulated as [Pd(1, 3-pn)(acac)]Cl (pn and acac stand... 相似文献
609.
Amandine Morot Sahar El Fekih Adeline Bidault Alizée Le Ferrand Albane Jouault Javid Kavousi Alexis Bazire Vianney Pichereau Alain Dufour Christine Paillard François Delavat 《Environmental microbiology》2021,23(9):5273-5288
Environmental Vibrio strains represent a major threat in aquaculture, but the understanding of their virulence mechanisms heavily relies on the transposition of knowledge from human-pathogen vibrios. Here, the genetic bases of the virulence of Vibrio harveyi ORM4 towards the European abalone Haliotis tuberculata were characterized. We demonstrated that luxO, encoding a major regulator of the quorum sensing system, is crucial for the virulence of this strain, and that its deletion leads to a decrease in swimming motility, biofilm formation, and exopolysaccharide production. Furthermore, the biofilm formation by V. harveyi ORM4 was increased by abalone serum, which required LuxO. The absence of LuxO in V. harveyi ORM4 yielded opposite phenotypes compared with other Vibrio species including V. campbellii (still frequently named V. harveyi). In addition, we report a full type III secretion system (T3SS) gene cluster in the V. harveyi ORM4 genome. LuxO was shown to negatively regulate the promoter activity of exsA, encoding the major regulator of the T3SS genes, and the deletion of exsA abolished the virulence of V. harveyi ORM4. These results unveil virulence mechanisms set up by this environmentally important bacterial pathogen and pave the way for a better molecular understanding of the regulation of its pathogenicity. 相似文献
610.
Elnaz Sadat Mirdamadi Zahra Khosrowpour Davod Jafari Mazaher Gholipourmalekabadi Mehran Solati-Hashjin 《Biotechnology and bioengineering》2023,120(3):836-851
The liver is one of the vital organs in the body, and the gold standard of treatment for liver function impairment is liver transplantation, which poses many challenges. The specific three-dimensional (3D) structure of liver, which significantly impacts the growth and function of its cells, has made biofabrication with the 3D printing of scaffolds suitable for this approach. In this study, to investigate the effect of scaffold geometry on the performance of HepG2 cells, poly-lactic acid (PLA) polymer was used as the input of the fused deposition modeling (FDM) 3D-printing machine. Samples with simple square and bioinspired hexagonal cross-sectional designs were printed. One percent and 2% of gelatin coating were applied to the 3D printed PLA to improve the wettability and surface properties of the scaffold. Scanning electron microscopy pictures were used to analyze the structural properties of PLA–Gel hybrid scaffolds, energy dispersive spectroscopy to investigate the presence of gelatin, water contact angle measurement for wettability, and weight loss for degradation. In vitro tests were performed by culturing HepG2 cells on the scaffold to evaluate the cell adhesion, viability, cytotoxicity, and specific liver functions. Then, high-precision scaffolds were printed and the presence of gelatin was detected. Also, the effect of geometry on cell function was confirmed in viability, adhesion, and functional tests. The albumin and urea production of the Hexagonal PLA scaffold was about 1.22 ± 0.02-fold higher than the square design in 3 days. This study will hopefully advance our understanding of liver tissue engineering toward a promising perspective for liver regeneration. 相似文献