全文获取类型
收费全文 | 139篇 |
免费 | 10篇 |
出版年
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2013年 | 3篇 |
2012年 | 3篇 |
2011年 | 10篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 5篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 8篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 4篇 |
1965年 | 1篇 |
1964年 | 2篇 |
1934年 | 1篇 |
1932年 | 1篇 |
排序方式: 共有149条查询结果,搜索用时 672 毫秒
141.
142.
143.
144.
Benu Sethi Monika Jain Manish Chowdhary Yogesh Soni Yukti Bhatia Vikram Sahai Saroj Mishra 《Biotechnology and Bioprocess Engineering》2002,7(1):43-51
The cloning and expression of β-glucosidase II, encoded by the geneßglu2, from thermotolerant yeastPichia etchellsii intoEscherichia coli is described. Cloning of the 7.3 kbBamHI/SalI yeast insert containingßglu2 in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid intoE. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At 50°C, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of 0.14 h?1. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies. 相似文献
145.
Application of some Multivariate Analysis Techniques to Data on Effect of Air-Pollution on Morbidity
H. Sahai 《Biometrical journal. Biometrische Zeitschrift》1978,20(6):557-573
In this article an attempt has been made to analyze the data obtained from an air-pollution study to investigate the effects of the levels of air pollution exposure in causing morbidity, using some multivariate statistical techniques. Considering the importance of the theoretical aspect of the problem, the usual theory related to the application of these methods has also been described. 相似文献
146.
147.
148.
Hiroshi Kondo Colin D.H. Ratcliffe Steven Hooper James Ellis James I. MacRae Marc Hennequart Christopher W. Dunsby Kurt I. Anderson Erik Sahai 《Cell reports》2021,34(7):108750
- Download : Download high-res image (168KB)
- Download : Download full-size image
149.
S. Suresh Kumar Lalit Kumar Vikram Sahai Rani Gupta 《Journal of industrial microbiology & biotechnology》2009,36(3):427-432
An alkaline lipase from Trichosporon asahii MSR 54 was used to develop presoak formulation for removing oil stains at ambient temperature. The lipase was produced in
a reactor followed by concentration by ultrafiltration and then it was dried with starch. The biochemical characteristics
of enzyme showed that it was an alkaline lipase having pH activity in the range of pH 8.0–10.0 and temperature in the range
of 25–50°C. The present lipase was active >80% at 25°C. The lipase was cystein activated with fourfold enhancement in presence
of 5 mM cystein and likewise the activity was also stimulated in presence of papain hydrolysate which served as source of
cystein. The presoak formulation consisted of two components A and B, component A was enzyme additive and B was a mixture
of carbonate/bicarbonate source of alkali and papain hydrolysate as source of cystein. The results indicated that the presoaking
in enzyme formulation followed by detergent washing was a better strategy for stain removal than direct washing with detergent
in presence of lipase. Further, it was observed that 0.25% presoak component B in presence of 100 U enzyme component A (0.1 g)
was the best formulation in removing maximum stain from mustard oil/triolein soiled clothes as indicated by increase in reflectance
which was found equal to that of control cloth. The lipase action in presoaked formulation was clearly indicated by quantitated
fatty acid release and also the TLC results of wash water, where oil hydrolytic products were visible only in presence of
enzyme in the treatment. The wash performance carried at 25°C indicated that washing at 25°C was at par with that at 40°C
as indicated by similar reflectance of the washed cloth piece though qualitative fatty acid release was higher at 40°C. 相似文献