全文获取类型
收费全文 | 139篇 |
免费 | 10篇 |
出版年
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2016年 | 1篇 |
2015年 | 3篇 |
2013年 | 3篇 |
2012年 | 3篇 |
2011年 | 10篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 7篇 |
2007年 | 5篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 8篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 4篇 |
1965年 | 1篇 |
1964年 | 2篇 |
1934年 | 1篇 |
1932年 | 1篇 |
排序方式: 共有149条查询结果,搜索用时 562 毫秒
131.
V Kumar MV Sarma K Saharan R Srivastava L Kumar V Sahai VS Bisaria AK Sharma 《World journal of microbiology & biotechnology》2012,28(2):595-603
In the present investigation, the effect of three beneficial organisms (root endophytic fungus Piriformospora indica (Pi) and pseudomonads strains R62 and R81) and their four different consortia (Pi+R62, Pi+R81, R62+R81, Pi+R62+R81) was investigated
on the plant Vigna mungo through their inorganic carrier-based (talcum powder and vermiculite) formulations. All the treatments resulted in significant
increase in growth parameters under glasshouse as well as field conditions and showed a consistency in their performance on
moving from glasshouse to field conditions. In glasshouse conditions, a maximum increase of 4.5-fold in dry root weight and
3.9-fold in dry shoot weight compared to control was obtained with vermiculite-based consortium formulation of Pi+R81. In
field studies using vermiculite as carrier, a maximum enhancement of 3.2-fold in dry root weight, 3.0-fold in dry shoot weight,
8.4-fold in number of nodules and 4.0-fold in number of pods in comparison to control was obtained with the bio-inoculant
formulation containing consortium of Pi+R81. The same treatment also caused the highest improvement of 1.9-fold in nitrogen
content and 1.7-fold in phosphorus content, while the highest increase of 1.4-fold in potassium content was obtained with
Pi alone. 相似文献
132.
133.
Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production 总被引:1,自引:0,他引:1
A chemically defined medium was optimized for the maximum biomass production of recombinant Pichia pastoris in the fermentor cultures using glycerol as the sole carbon source. Optimization was done using the statistical methods for getting the optimal level of salts, trace metals and vitamins for the growth of recombinant P. pastoris. The response surface methodology was effective in optimizing nutritional requirements using the limited number of experiments. The optimum medium composition was found to be 20 g/L glycerol, 7.5 g/L (NH4)2SO4, 1 g/L MgSO4.7H2O, 8.5 g/L KH2PO4, 1.5 mL/L vitamin solution and 20 mL/L trace metal solution. Using the optimized medium 11.25 g DCW/L biomass was produced giving a yield coefficient of 0.55 g biomass/g of glycerol in a batch culture. Chemostat cultivation of recombinant P. pastoris was done in the optimized medium at different dilution rates to determine the kinetic parameters for growth on glycerol. Maximum specific growth rate of 0.23 h(-1) and Monod saturation constant of 0.178 g/L were determined by applying Monod model on the steady state data. Products of fermentation pathway, ethanol and acetate, were not detected by HPLC even at higher dilution rates. This supports the notion that P. pastoris cells grow on glycerol by a respiratory route and are therefore an efficient biomass and protein producers. 相似文献
134.
135.
136.
The effect of three organochlorinated pesticides on the gill structure of Puntius ticto, a freshwater teleost, was investigated. Fish exposed to sublethal concentrations of BHC (0.17 ppm), lindane (0.19 ppm) and endosulfan (0.20 ppm) were studied. The pesticides were detected qualitatively in the gill tissue by thin-layer chromatography (TLC). The results showed that they could be detected after 15 days' exposure, but not after 96 h exposure. Histopathological examination revealed several structural and functional changes in the gills. Exposure to BHC was followed by an inflammatory reaction and complete dystrophy of the lamellar structure of the gills. Lindane-treated fish showed disruption of the epithelial covering of the gills and excessive haemorrhage in the blood vessels. In exposure to endosulfan the gill lamellae shrank and became thinner. 相似文献
137.
138.
139.
Summary The (Ca2+ + Mg2+) ATPase which serves as a Ca2+ pump in the kidney basolateral membranes is essential to the maintenance of an intracellular Ca2+ concentration optimal for kidney function. Since atrial natriuretic peptide (ANP) is known to participate in the Ca2+ homeostasis mechanism, altered levels of ANP in diabetes may vary the pump activity and consequently the kidney function. In order to examine the modulatory role of ANP on (Ca2+ + Mg2+) ATPase in short- (6 weeks) and long-term (6 months) diabetes, rats were injected with streptozotocin (65 mg/kg body wt, i.v.). At 6 weeks, the plasma ANP was decreased whereas, ANP-receptor binding in the kidney basolateral membrane was increased. In contrast, there was an increased plasma ANP and decreased ANP receptor binding at 6 months. Insulin treatment to diabetic animals normalized these parameters. The (Ca2+ + Mg2+) ATPase activity was unchanged both at 6 weeks and 6 months. Our results demonstrate that the unchanged Ca2+ pump activity in short-term and long-term diabetes serves to maintain the Ca2+ homeostasis in the kidney cells and thus may maintain the hyperfiltration state in diabetes. Unaltered (Ca2+ + Mg2+) ATPase is achieved by the initial up-regulation and subsequent down-regulation of the ANP receptors. 相似文献
140.