排序方式: 共有11条查询结果,搜索用时 0 毫秒
11.
Two-dimensional electrophoretic proteome study of serum thermostable fraction from patients with various tumor conditions 总被引:2,自引:0,他引:2
Goufman EI Moshkovskii SA Tikhonova OV Lokhov PG Zgoda VG Serebryakova MV Toropygin IY Vlasova MA Safarova MR Makarov OV Archakov AI 《Biochemistry. Biokhimii?a》2006,71(4):354-360
One of the problems of plasma proteomics is a presence of large major components. In this work, we use the thermostable fraction as a way to deplete these major proteins. The thermostable fraction of serum samples from patients with ovarian, uterus, and breast cancers and benign ovarian tumor was analyzed using two-dimensional electrophoresis combined with MALDI-TOF(-TOF)-mass spectrometry. Of them, alpha-1-acid glycoprotein and clusterin are expressly down-regulated in breast cancer, whereas transthyretin is decreased specifically in ovarian cancer. Apolipoprotein A-I forms have decreased spot volumes, while haptoglobin alpha1, in contrast, is elevated in several tumors. These data are partly consistent with previous art studies on cancer proteomics, which involve mass-spectrometry-based serum profiling techniques. Serum thermostable fraction may be recommended as a good tool for medium and small protein proteome investigation, in particular, by 2D-electrophoresis. 相似文献