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51.
Abdi Sheyda Montazeri Vahid Garjani Alireza Shayanfar Ali Pirouzpanah Saeed 《Molecular biology reports》2020,47(4):2459-2473
Molecular Biology Reports - Low levels of coenzyme Q10 (CoQ10) have been reported in the circulation of patients with breast cancer, particularly in metastatic features. Our objective was to study... 相似文献
52.
Zahra Farzaneh Saeed Abbasalizadeh Mohammad-Hassan Asghari-Vostikolaee Mehdi Alikhani Joaquim M. S. Cabral Hossein Baharvand 《Biotechnology and bioengineering》2020,117(12):3739-3756
Developing technologies for scalable production of human organoids has gained increased attention for “organoid medicine” and drug discovery. We developed a scalable and integrated differentiation process for generation of hepatic organoid from human pluripotent stem cells (hPSCs) in a fully controlled stirred tank bioreactor with 150 ml working volume by application of physiological oxygen concentrations in different liver tissue zones. We found that the 20–40% dissolved oxygen concentration [DO] (corresponded to 30–60 mmHg pO2 within the liver tissue) significantly influences the process outcome via regulating the differentiation fate of hPSC aggregates by enhancing mesoderm induction. Regulation of the [DO] at 30% DO resulted in efficient generation of human fetal-like hepatic organoids that had a uniform size distribution and were comprised of red blood cells and functional hepatocytes, which exhibited improved liver-specific marker gene expressions, key liver metabolic functions, and, more important, higher inducible cytochrome P450 activity compared to the other trials. These hepatic organoids were successfully engrafted in an acute liver injury mouse model and produced albumin after implantation. These results demonstrated the significant impact of the dissolved oxygen concentration on hPSC hepatic differentiation fate and differentiation efficacy that should be considered ascritical translational aspect of established scalable liver organoid generation protocols for potential clinical and drug discovery applications. 相似文献
53.
Milad Ashrafizadeh Zahra Ahmadi Tahereh Farkhondeh Saeed Samarghandian 《Journal of cellular physiology》2020,235(4):3157-3168
Autophagy is considered as an important mechanism for maintaining homeostasis and responsible for the degradation of superfluous or potentially toxic components and organelles. Autophagy impairment is associated with a number of pathological conditions, such as aging, neurological disorders, cancer, and infection. Autophagy also plays a significant role in cancer chemotherapy. The multiple cancer drugs have been notably developed with the strategy of autophagy modulation. Statins, 3-hydroxy-3-methyl-glutaryl-CoA inhibitors, are known due to their efficacy in decreasing low-density lipoprotein and extensively used for the management of cardiovascular diseases. Statins have other therapeutic and biological activities, such as antioxidant, anti-inflammatory, antitumor, and neuroprotective known as pleiotropic effects. It seems that statins are capable of targeting various signaling pathways in the induction of their great pharmacological effects. At the present study, we demonstrate the therapeutic effects of statins mediated via autophagy regulation. 相似文献
54.
Zahra Kiani Doostmorad Zafari Saeed Rezaee Amir Arjmandian Mazdasht Gitti P.C. Struik 《Archives Of Phytopathology And Plant Protection》2013,46(19):2401-2408
Globodera rostochiensis and Rhizoctonia solani are the most important growth limiting factors influencing potato production in Iran. The effects of inoculation with Potato Cyst Nematodes (PCN) (0, 50, 75 and 100 cysts/3.5?kg soil) and R. solani (with or without inoculation) on potato growth and development were investigated in cultivars Santé and Marfona. Inoculation with R. solani induced severe damage, especially when inoculation was accompanied with high density of PCN. The damage caused by R. solani tended to increase with an increase in PCN density, especially in Marfona. In Santé, number of stems or branches per plant significantly increased by inoculation with R. solani, while in Marfona it was significantly affected either by R. solani inoculation or PCN density. In Santé, number of stolons per plant was significantly increased by PCN, but not by R. solani. In Marfona, however, the number of stolons per plant was significantly affected either by R. solani inoculation or by presence of PCN, but not affected by PCN density. The general effect of R. solani or PCN inoculation treatments on shoot, below-ground and total dry weight of potato was significant, but strongly affected by cultivar. In general, our study supports the synergistic interaction between R. solani and PCN and its moderation by the use of a resistant cultivar such as Santé. 相似文献
55.
Padideh Ebadi Saeed Daneshmandi Abbas Ghasemi Mohammad Hossein Karimi 《Molecular biology reports》2013,40(11):6255-6260
Gallstone is a common biliary disorder with several risk factors. Immune responses and inflammatory cytokines are important in this disease; as a result, some cytokines can be detected in bile fluid. In this research, cytokine gene polymorphisms were studied, and their effects on gallstone formation were evaluated. On 158 gallstone patients and 254 normal subjects, by PCR- RFLP method, IL-4-C590T polymorphism and by ARMS-PCR method, IFN-γ T+874A, TNF-α-A308G, IL-6 G-174C and TGF-β T+869C variants were studied. Pathologic evaluations were done on surgical specimens. There were no significant differences in distribution of evaluated polymorphisms between patient group and normal control group (P > 0.05), except TGF-β +869T allele (P = 0.04, OR = 1.23, 95 % CI = 1–1.79) which was higher in patients with gallstone. Although the pro-inflammatory cytokines such as TNF-α and IL-6 may promote gallstone formation, in this study no significant correlation between TNF-α and IL-6 polymorphisms and gallstone formation was seen. It is taught that TGF-β may affect gallbladder cells to promote gallstone formation and higher producer TGF-β +869T allele can be a risk factor of gallstone disease, so further studies would be more elucidative. 相似文献
56.
Mohammad Reza Noori-Daloii Nazanin Jalilian Pantea Izadi Maryam Sobhani Zeinab Rabii-Gilani Mir Saeed Yekaninejad 《Molecular biology reports》2013,40(8):4861-4867
Graft versus host disease (GVHD) is a major complication of bone marrow transplantation (BMT). Numerous studies have shown the potential role of cytokine genotypes in the occurrence of GVHD. In this retrospective, case–control study we aimed to investigate the association between 13 cytokine genes and acute GVHD (aGVHD) after HLA-identical sibling BMT in 91 Iranian subjects. Negative association was found between aGVHD and donor IL-10/GCC haplotype or donor IL-4Ra-A allele in the population study. When compared within the leukemia subgroup, we observed positive association between recipient IL-1α ?889/C allele and aGVHD. Also there were negative association between recipient IL-10/CAA haplotype and donor IL-4Ra/A allele and development of aGVHD. Among the different genotypes only donor IL-4Ra and donor IL-12 showed significant association. We conclude that several cytokine polymorphisms are positively and negatively associated with aGVHD in Iranian HLA matched siblings, of which IL-4Ra and IL-12 may play important roles. 相似文献
57.
Aseel J. Marzoq Nathalia Giese J?rg D. Hoheisel Mohamed Saiel Saeed Alhamdani 《The Journal of biological chemistry》2013,288(45):32517-32527
Pancreatic stellate cells are key mediators in chronic pancreatitis and play a central role in the development of pancreatic fibrosis, stromal formation, and progression of pancreatic cancer. This study was aimed at investigating molecular changes at the level of the proteome that are associated with the activation of pancreatic stellate cells by proinflammatory factors, namely TNF-α, FGF2, IL6, and chemokine (C-C motif) ligand 4 (CCL4). They were added individually to cells growing in serum-free medium next to controls in medium supplemented with serum, thus containing a mixture of them all, or in serum-free medium alone. Variations were detected by means of a microarray of 810 antibodies targeting relevant proteins. All tested factors triggered increased proliferation and migration. Further analysis showed that TNF-α is the prime factor responsible for the activation of pancreatic stellate cells. CCL4 is associated with cellular neovascularization, whereas FGF2 and IL6 induction led to better cellular survival and decreased apoptotic activity of the stellate cells. The identified direct effects of individual cytokines on human pancreatic stellate cells provide new insights about their contribution to pancreatic cancer promotion. 相似文献
58.
A hitherto unknown species of the dipteran family Ulidiidae is described from Iran and Turkey: Herina rajabii n. sp. is similar to H. gyrans (Loew) and H. tristis (Meigen), differing from them by the yellowish-brown scutellum and presence of a very strong subcercal prensiseta on the surstylus. A key to the species of the Herina tristis-group of species is provided. 相似文献
59.
Seyyed Saeed HOSSEINIAN YOUSEFKHANI Gentile Francesco FICETOLA Nasrullah RASTEGAR-POUYANI Natalia B ANANJEVA Eskandar RASTEGAR-POUYANI Rafaqat MASROOR 《亚洲两栖爬行动物研究(英文版)》2013,(3):207-213
Predictive potential distribution modeling is crucial in outlining habitat usage and establishing conservation management priorities. In this paper we provide detailed data on the distribution of the Caucasian rock agama Para- laudakia caucasia, and use species distribution models (MAXENT) to evaluate environmental suitability and potential distribution at a broad spatial scale. Locality data on the distribution of P. caucasia have been gathered over nearly its entire range by various authors from field surveys. The distribution model ofP caucasia showed good performance (AUC = 0.887), and predicted high suitability in regions mainly located in Tajikistan, north Pakistan, Afghanistan, southeast Turkmenistan, northeast Iran along the Elburz mountains, Transcaueasus (Azerbajan, Armenia, Georgia), northeastern Turkey and northward along the Caspian Sea coast in Daghestan, Russia. The identification of suitable areas for this species will help to assess conservation status of the species, and to set up management programs. 相似文献