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51.
Muhammad Waseem M.Amin Ullah Shah Rizwana Aleem Qureshi Iqbal Muhammad Rabia Afza Saeeda Yousaf 《Plant Diversity》2006,28(5):535-542
The present paper represents the ethnopharmacological survey of Sudhan Gali, Kashmir, Pakistan. The study revealed that 12 plant species belonging to 11 families were used for the treatment of stomach, diabetes and ophthalmic diseases by the local people in Sudhan Gali. Achillea millefolium , Aconitum heterophyllum, Berberis lycium, Polygonum amplexicaule, Mentha longifolia, Paeonia emodi, Plantago lanceolata were locally used for stomach related problems treatment; Berberis lycium, Skimmia lareola, Solanum dulcamara for diabetes and Geranium wallichianum, Artemisia vulgaris, Solanum dulcamara, and Corydalis crassifolia used for the treatment of ophthalmic diseases. Two species Berberis lycium and Solanum dulcamara have multipurpose value. Former is used to treat stomach as well as diabetes while latter is used to treat not only to diabetes but also ophthalmic diseases. According to IUCN categories , out of these 12 plant species collected and marketed, Polygonum amplexicaule and Paeonia emodi are endangered, Aconitum heterophyllum; Berberis lycium species are vulnerable while Plantago lanceolata and Skimmia lareola species are rare.The availability of these medicinal plants has decreased during the past 20 years and these are facing a drastic biotic pressure due to their extensive usage and non-scientific methods of collection. It is quite evident that these valuable native medicinal plants species are going to decline in number and ultimately will become extinct if no timely proper conservation strategies are adopted. 相似文献
52.
This study examines and quantifies the linkages between population health, environmental risks, and its determinants for drinking
water in New Zealand using routinely collected data. It was conducted as part of the national environmental health indicators
project in New Zealand. The project is based on the World Health Organization’s (WHO) “Environmental and Health Information
System” program. Drinking water quality indicators based on the Driving force–Pressure–State–Exposure–Effect–Action (DPSEEA)
framework as part of this program were analyzed to validate the model by quantifying the linkages between the indicators.
The results of the model suggested over the study period, the state (drinking water quality) and exposure (water access) indicators
are significant independent predictors of the effect indicator (waterborne disease rate). This study suggests that routinely
collected data can be structured using the DPSEEA framework and tested quantitatively using standard Poisson regression models,
thus, illustrating that the model can be used routinely to provide a basis for consideration of the costs and benefits of
any interventions to reduce the burden of waterborne disease. Data quality issues need to be considered if such routinely
collected data linkages are to be performed for policy purposes.
The online version of this article (doi:) contains electronic supplementary material, which is available to authorized users.
Supplemental histograms are available in the online appendix. 相似文献
53.
Elisa Migliorini Dhruv Thakar Jens Kühnle Rabia Sadir Douglas P. Dyer Yong Li Changye Sun Brian F. Volkman Tracy M. Handel Liliane Coche-Guerente David G. Fernig Hugues Lortat-Jacob Ralf P. Richter 《Open biology》2015,5(8)
The glycosaminoglycan heparan sulfate (HS), present at the surface of most cells and ubiquitous in extracellular matrix, binds many soluble extracellular signalling molecules such as chemokines and growth factors, and regulates their transport and effector functions. It is, however, unknown whether upon binding HS these proteins can affect the long-range structure of HS. To test this idea, we interrogated a supramolecular model system, in which HS chains grafted to streptavidin-functionalized oligoethylene glycol monolayers or supported lipid bilayers mimic the HS-rich pericellular or extracellular matrix, with the biophysical techniques quartz crystal microbalance (QCM-D) and fluorescence recovery after photobleaching (FRAP). We were able to control and characterize the supramolecular presentation of HS chains—their local density, orientation, conformation and lateral mobility—and their interaction with proteins. The chemokine CXCL12α (or SDF-1α) rigidified the HS film, and this effect was due to protein-mediated cross-linking of HS chains. Complementary measurements with CXCL12α mutants and the CXCL12γ isoform provided insight into the molecular mechanism underlying cross-linking. Fibroblast growth factor 2 (FGF-2), which has three HS binding sites, was also found to cross-link HS, but FGF-9, which has just one binding site, did not. Based on these data, we propose that the ability to cross-link HS is a generic feature of many cytokines and growth factors, which depends on the architecture of their HS binding sites. The ability to change matrix organization and physico-chemical properties (e.g. permeability and rigidification) implies that the functions of cytokines and growth factors may not simply be confined to the activation of cognate cellular receptors. 相似文献
54.
Vesicular stomatitis virus (VSV) was incubated with pyrene dodecanoic acid (P12), a fluorescent derivative of a medium-chain length fatty acid, and subjected to irradiation with a long wavelength ultra-violet light source at 366 nm (UVA). This inactivated the virus, resulting in a drastic decrease of its infectivity. The virus inactivation was proportional to the concentration of the pyrene-fatty acid, the length of exposure of the virus to P12 and the irradiation dose. 相似文献
55.
Budama-Kilinc Yasemin Kecel-Gunduz Serda Cakir-Koc Rabia Aslan Bahar Bicak Bilge Kokcu Yagmur Ozel Aysen E. Akyuz Sevim 《International journal of peptide research and therapeutics》2021,27(3):2015-2028
International Journal of Peptide Research and Therapeutics - The aim of the current study was to design a drug delivery nano-system of natural growth-modulating peptide known as GHK that naturally... 相似文献
56.
Differential T cell trafficking through the blood compartment towards infected foci may be occurring in different stages of tuberculosis disease and infection. The aim of the present study was to identify cytokine signatures in the blood compartment in tuberculosis patients with pulmonary disease (PTB=19), recently exposed household contacts (HC=27) and nonexposed community controls (EC=37). Diluted (1:10) whole blood was cultured for 2 days and cytokine secretion was assessed using Cytometric Bead Array (Th1/Th2 kit II; BD Biosciences) which included IL-2, TNF-α, IFN-γ (Type1/T1), IL-4, IL-6 and IL-10 (Type2/T2). All T1/T2 cytokines were elevated in PTB (AUROC>0.9) while HC showed selective elevation of IL-6 (AUROC>0.7) compared to EC. Principal component analysis (PCA) extracted two groupings with Eigen values >1; IL-6 separated into the second component for PTB, HC and EC. After rotation, IFN-γ was correlated with the first component for PTB and EC and the second component for HC indicating an absence of T1/T2 dichotomy. Therefore endogenous cytokine signatures may indicate differential T cell trafficking in different stages of tuberculosis infection and disease. 相似文献
57.
58.
Helling S Hüttemann M Ramzan R Kim SH Lee I Müller T Langenfeld E Meyer HE Kadenbach B Vogt S Marcus K 《Proteomics》2012,12(7):950-959
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial electron transport chain, is regulated by isozyme expression, allosteric effectors such as the ATP/ADP ratio, and reversible phosphorylation. Of particular interest is the "allosteric ATP-inhibition," which has been hypothesized to keep the mitochondrial membrane potential at low healthy values (<140 mV), thus preventing the formation of superoxide radical anions, which have been implicated in multiple degenerative diseases. It has been proposed that the "allosteric ATP-inhibition" is switched on by the protein kinase A-dependent phosphorylation of COX. The goal of this study was to identify the phosphorylation site(s) involved in the "allosteric ATP-inhibition" of COX. We report the mass spectrometric identification of four new phosphorylation sites in bovine heart COX. The identified phosphorylation sites include Tyr-218 in subunit II, Ser-1 in subunit Va, Ser-2 in subunit Vb, and Ser-1 in subunit VIIc. With the exception of Ser-2 in subunit Vb, the identified phosphorylation sites were found in enzyme samples with and without "allosteric ATP inhibition," making Ser-2 of subunit Vb a candidate site enabling allosteric regulation. We therefore hypothesize that additional phosphorylation(s) may be required for the "allosteric ATP-inhibition," and that these sites may be easily dephosphorylated or difficult to identify by mass spectrometry. 相似文献
59.
60.
We propose a new mathematical model that describes the growth of fibrous tissue around rigid, disk-shaped implants. A solution methodology based on an efficient regularized iterative method is presented to calibrate the model from some measurements of the capsule tissue concentration. Numerical results obtained with synthetic data are presented to demonstrate the ability of the proposed solution methodology to determine the model parameters corresponding to a given implant. In addition, numerical results obtained with experimental data are presented to illustrate the validity of the proposed model. 相似文献