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191.
Terasaki N Yamamoto N Tamada K Hattori M Hiraga T Tohri A Sato I Iwai M Iwai M Taguchi S Enami I Inoue Y Yamanoi Y Yonezawa T Mizuno K Murata M Nishihara H Yoneyama S Minakata M Ohmori T Sakai M Fujii M 《Biochimica et biophysica acta》2007,1767(6):653-659
We report on the first successful output of electrons directly from photosystem I (PSI) of thermophilic cyanobacteria to the gate of a field-effect transistor (FET) by bypassing electron flow via a newly designed molecular wire, i.e., artificial vitamin K(1), and a gold nanoparticle; in short, this newly manufactured photosensor employs a bio-functional unit as the core of the device. Photo-electrons generated by the irradiation of molecular complexes composed of reconstituted PSI on the gate were found to control the FET. This PSI-bio-photosensor can be used to interpret gradation in images. This PSI-FET system is moreover sufficiently stable for use exceeding a period of 1 year. 相似文献
192.
Nao Terasaki Kaoru Tamada Takashi Hiraga Akihiko Tohri Masako Iwai Shunpei Taguchi Yasunori Inoue Yoshinori Yamanoi Katsuya Mizuno Hiroshi Nishihara Satoshi Yoneyama Tsutomu Ohmori Masaaki Fujii 《BBA》2007,1767(6):653-659
We report on the first successful output of electrons directly from photosystem I (PSI) of thermophilic cyanobacteria to the gate of a field-effect transistor (FET) by bypassing electron flow via a newly designed molecular wire, i.e., artificial vitamin K1, and a gold nanoparticle; in short, this newly manufactured photosensor employs a bio-functional unit as the core of the device. Photo-electrons generated by the irradiation of molecular complexes composed of reconstituted PSI on the gate were found to control the FET. This PSI-bio-photosensor can be used to interpret gradation in images. This PSI-FET system is moreover sufficiently stable for use exceeding a period of 1 year. 相似文献
193.
Kazunori Taguchi Yoshihiro Aoyagi Hiromi Matsusaki Toshiaki Fukui Yoshiharu Doi 《Biotechnology letters》1999,21(7):579-584
A Pseudomonas sp. 61-3 malonyl-CoA-ACP transacylase gene (fabD
Ps) was cloned by Southern analysis using an equivalent gene of Escherichia coli (fabD
Ec) as a probe. Some recombinant E. coli HB101 strains harboring fabD
Ps, fabD
Ec, or E. coli 3-ketoacyl-ACP synthase III gene (fabH
Ec) with Aeromonas caviae polyhydroxyalkanoate synthase gene (phaC
Ac) were constructed and grown on one-stage cultivation in Luria-Bertani broth containing glucose as carbon source. These strains accumulated 5 to 11 wt% of poly (3-hydroxybutyrate) (PHB) within cells. Over-expression of fabH
Ec, fabD
Ec, or fabD
Ps has been suggested to lead the monomer-supply of (R)-3-hydroxybutyryl-CoA for PHB synthesis in E. coli cells. 相似文献
194.
Short communication. Biomass and production of cyanobacteria in a coastal water of Sagami Bay, Japan
Hamasaki K; Satoh F; Kikuchi T; Toda T; Taguchi S 《Journal of plankton research》1999,21(8):1583-1591
Cyanobacteria were relatively small contributors to carbon biomass
(097-18%) in the euphotic zone. However, a higher contribution to
production obtained at the surface water (16-45%) implies that they
contribute more to carbon cycling than is expected from their biomass
相似文献
195.
M. Okochi T. Taguchi M. Tsuboi N. Nakamura T. Matsunaga 《Applied microbiology and biotechnology》1999,51(3):364-369
A rapid method for the direct measurement of viable and dead adhering diatoms was developed using a fluorescent dye, TO-PRO-1
iodide. By staining the marine diatom, Nitzchia closterium, with TO-PRO-1 iodide, viable and dead cells were identified as red and yellow cells respectively, under an epifluorescence
microscope employing blue excitation. Only dead cells were stained with TO-PRO-1 iodide. Viable cells were observed as red
because of autofluorescence arising from intracellular chlorophyll, whereas dead cells were observed as yellow because of
the fluorescence of TO-PRO-1 iodide. The percentage of TO-PRO-1-iodide-stained was correlated with the percentage of dead
cells in N. closterium cells exposed to heat (60 °C, 15 min). Other microalgae containing intracellular chlorophyll could be also distinguished
as viable or dead cells by this fluorometric staining method. This method was applied for the assessment of N. closterium cells killed by the electrochemical treatment and used to monitor biofouling populations and their viability directly on
the electrode surface. When 1.0 V was applied against a saturated calomel electrode, 99% of the cells attached to graphite
electrode were killed in 1 h.
Received: 7 August 1998 / Received revision: 16 October 1998 / Accepted: 7 November 1998 相似文献
196.
Suguru N. Kudoh Kazuyuki Kiyosue Michiki Kasai Takahisa Taguchi 《Cellular and molecular neurobiology》1999,19(5):575-585
1.We reported in a previous paper that long-lasting enhancement of spontaneous excitatory post synaptic currents (SEPSCs) in cultured chick cerebral neurons was induced by exposure to a conditioned medium (CM) prepared by Mg2+-free treatment of neurons. This suggested that the CM contained a diffusible factor(s) for the potentiation.2.In this paper, the factor(s) was shown to be a protein(s) by heat and trypsin treatment of the CM.3.The factor induced the potentiation within 5 min, but it was not required for maintenance of increased SEPSCs.4.The factors in CM induced the potentiation without protein synthesis.5.Protein synthesis at least in postsynaptic neurons, was indispensable to induce the potentiation by the Mg2+-free condition. 相似文献
197.
198.
Sitara D Kim S Razzaque MS Bergwitz C Taguchi T Schüler C Erben RG Lanske B 《PLoS genetics》2008,4(8):e1000154
Maintenance of physiologic phosphate balance is of crucial biological importance, as it is fundamental to cellular function, energy metabolism, and skeletal mineralization. Fibroblast growth factor-23 (FGF-23) is a master regulator of phosphate homeostasis, but the molecular mechanism of such regulation is not yet completely understood. Targeted disruption of the Fgf-23 gene in mice (Fgf-23-/-) elicits hyperphosphatemia, and an increase in renal sodium/phosphate co-transporter 2a (NaPi2a) protein abundance. To elucidate the pathophysiological role of augmented renal proximal tubular expression of NaPi2a in Fgf-23-/- mice and to examine serum phosphate-independent functions of Fgf23 in bone, we generated a new mouse line deficient in both Fgf-23 and NaPi2a genes, and determined the effect of genomic ablation of NaPi2a from Fgf-23-/- mice on phosphate homeostasis and skeletal mineralization. Fgf-23-/-/NaPi2a-/- double mutant mice are viable and exhibit normal physical activities when compared to Fgf-23-/- animals. Biochemical analyses show that ablation of NaPi2a from Fgf-23-/- mice reversed hyperphosphatemia to hypophosphatemia by 6 weeks of age. Surprisingly, despite the complete reversal of serum phosphate levels in Fgf-23-/-/NaPi2a-/-, their skeletal phenotype still resembles the one of Fgf23-/- animals. The results of this study provide the first genetic evidence of an in vivo pathologic role of NaPi2a in regulating abnormal phosphate homeostasis in Fgf-23-/- mice by deletion of both NaPi2a and Fgf-23 genes in the same animal. The persistence of the skeletal anomalies in double mutants suggests that Fgf-23 affects bone mineralization independently of systemic phosphate homeostasis. Finally, our data support (1) that regulation of phosphate homeostasis is a systemic effect of Fgf-23, while (2) skeletal mineralization and chondrocyte differentiation appear to be effects of Fgf-23 that are independent of phosphate homeostasis. 相似文献
199.
Sumino M Ohkubo A Taguchi H Seio K Sekine M 《Bioorganic & medicinal chemistry letters》2008,18(1):274-277
5-Carboxy-2'-deoxycytidine (dC(COO-)) was synthesized as an anion-carrier to seek a new possibility of modified oligodeoxynucleotides capable of stabilization of duplexes and triplexes. The base pairing properties of this compound were evaluated by use of ab initio calculations. These calculations suggest that the Hoogsteen-type base pair of dC(COO-)-G is less stable than that of the canonical C+-G pair and the Watson-Crick-type base pair of dC(COO-)-G is slightly more stable than the natural G-C base pair. The modified cytosine base showed a basicity similar to that of cytosine (pKa 4.2). It turned out that oligodeoxynucleotides 13mer and 14mer incorporating dC(COO-) could form duplexes with the complementary DNA oligomer, which were more stable than the unmodified duplex. In contrast, it formed a relatively unstable triplex with the target ds DNA. 相似文献
200.
Fujibayashi A Taguchi T Misaki R Ohtani M Dohmae N Takio K Yamada M Gu J Yamakami M Fukuda M Waguri S Uchiyama Y Yoshimori T Sekiguchi K 《Cell structure and function》2008,33(1):35-50
RME-8 is a DnaJ-domain-containing protein that was first identified in Caenorhabditis elegans as being required for uptake of yolk proteins. RME-8 has also been identified in other species, including flies and mammals, and the phenotypes of their RME-8 mutants suggest the importance of this protein in endocytosis. In the present study, we cloned human RME-8 (hRME-8) and characterized its biochemical properties and functions in endocytic pathways. hRME-8 was found to be a peripheral protein that was tightly associated with the membrane via its N-terminal region. It partially colocalized with several early endosomal markers, but not with late endosomal markers, consistent with observations by immunoelectron microscopy. When cells were transfected with a panel of dominant-active Rab proteins, hRME-8 was confined to large vacuoles induced by expression of Rab5aQ79L, but not by Rab7Q67L. Expression of C-terminally-truncated hRME-8 mutants led to the formation of large puncta and vacuoles, and compromised endocytic pathways through early endosomes, i.e., recycling of transferrin and degradation of epidermal growth factor. Taken together, these results indicate that hRME is primarily involved in membrane trafficking through early endosomes, but not through degradative organelles, such as multivesicular bodies and late endosomes. 相似文献